LOCUS Exported 10420 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses Nfia and blasticidin resistance gene under control of tetO sequence.. ACCESSION . VERSION . KEYWORDS tetO.Nfia.Blast SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10420) AUTHORS Canals I, Ginisty A, Quist E, Timmerman R, Fritze J, Miskinyte G, Monni E, Hansen MG, Hidalgo I, Bryder D, Bengzon J, Ahlenius H TITLE Rapid and efficient induction of functional astrocytes from human pluripotent stem cells. JOURNAL Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2. Epub 2018 Aug 20. PUBMED 30127505 REFERENCE 2 (bases 1 to 10420) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10420 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..39 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 36..60 /label=LNCX /note="Human CMV promoter, forward primer" CDS 1693..1746 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 1759..2157 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 2196..2784 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2249..2269) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2667..2678) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(2787..2803) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2788..2804) /label=pBluescriptKS /note="For pBluescript vector" LTR 3309..3489 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(3515..3532) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 3521..3745 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3791..4219 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3878..3897) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4088..4109 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4228..4248) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4233..4562 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4413..4548 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4475..4494 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 4610..4657 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 4676..5050 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 5180..5301 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5217..5236) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5271..5290 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5350..5366) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5350..5366) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5363..5385) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5374..5390 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5398..5428) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5443..5464 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5581..5598) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5752..6340) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5832..5851) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6511..7371) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7134..7153 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7372..7476) /gene="bla" /label=AmpR promoter primer_bind complement(7551..7570) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 7742..8121 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 8123..8321 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 8276..8296 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 8339..8519 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8566..8691 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9184..9417 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9602..9646 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 9944..10061 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 10118..10388 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" ORIGIN 1 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc 61 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc 121 ctccgcggcc ccgaattcat gtattctccg ctctgtctca cccaggatga gtttcatcct 181 ttcattgaag cacttctgcc ccatgtccgc gccttcgcct acacatggtt caacctgcag 241 gcccgaaagc ggaaatactt caaaaaacat gagaagcgca tgtcgaaaga agaggagagg 301 gccgtgaagg atgaactgct aagcgagaag cccgaggtca agcagaagtg ggcttcccga 361 cttctggcca agttacggaa agatatccga cccgagtacc gagaggattt tgttcttaca 421 gttacaggga aaaaacctcc atgctgtgtt ctttccaacc ctgatcagaa aggcaagatg 481 cggagaattg actgcctccg ccaggcagat aaagtatgga ggttggacct cgtcatggtg 541 atcttgttca aaggtattcc gctggaaagt actgatggcg agcgccttgt aaagagtcca 601 cagtgctcta atccagggct ctgtgtccag ccccatcaca taggggtttc tgtaaaggaa 661 ctcgatttat atttggcata ctttgtacat gcagcagatt caagtcaatc tgaaagtccc 721 agccagccaa gtgaagctga cattaaggac cagccagaaa atggacattt gggcttccag 781 gacagctttg tcacatcagg tgttttcagt gtgactgagc tagtaagagt gtcacaaaca 841 ccaatagctg caggaaccgg ccccaatttt tctctctctg atttggaaag ttcttcatac 901 tacagcatga gtccaggagc aatgaggagg tctctgccca gcacatcctc taccagctct 961 acaaagcgcc tcaagtctgt ggaggatgaa atggacagtc ctggtgaaga accattttac 1021 acaggccaag ggcgctcccc agggagtggc agccagtcca gtggatggca tgaagtagag 1081 ccaggcttgc catctccaag cacactgaag aagtctgaga agtctggttt cagcagcccc 1141 tccccttcgc agacctcctc cctgggaaca gcattcacac agcatcaccg acctgtcatt 1201 acaggaccca gagcaagtcc acatgcgacg ccatcgactc tccactttcc aacgtcaccc 1261 atcatccagc agcctgggcc ttacttctca cacccagcca tccgttacca ccctcaggag 1321 acgctgaaag agtttgtcca acttgtctgt cctgatgctg gtcagcaagc tggacaggtg 1381 gggttcctca atcccaatgg aagcagtcaa ggcaaggtgc acaacccatt cctccccacc 1441 ccaatgttgc cgccgccgcc accaccaccg atggccaggc ctgtgcctct gcccatgcca 1501 gacaccaagc ctccaaccac atcaacagaa ggaggtgcag cctcccccac ctcaccgacc 1561 tactcgacac ccagcacctc ccccgcaaac cgattcgtca gtgttggacc acgagatcca 1621 agctttgtaa atatccctca acagacacag tcctggtacc tgggataagg atccatggct 1681 agcggctccg gcgagggcag gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc 1741 ggcccagcat gctctagaat ggccaagcct ttgtctcaag aagaatccac cctcattgaa 1801 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca 1861 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga 1921 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact 1981 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga 2041 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag 2101 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaatta 2161 attaagctag ctaattcgat atcaagctta tcgataatca acctctggat tacaaaattt 2221 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 2281 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 2341 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 2401 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 2461 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 2521 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 2581 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 2641 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 2701 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 2761 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg 2821 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac 2881 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt 2941 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 3001 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact 3061 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat 3121 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga 3181 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat 3241 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg 3301 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 3361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 3421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 3481 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 3541 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 3601 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 3661 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 3721 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 3781 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 3841 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 3901 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 3961 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 4021 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 4081 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 4141 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 4201 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 4261 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 4321 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 4381 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 4441 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 4501 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 4561 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta 4621 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc 4681 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt 4741 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt 4801 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac 4861 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt 4921 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg 4981 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga 5041 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg 5101 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 5161 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 5221 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 5281 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 5341 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 5401 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 5461 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 5521 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 5581 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 5641 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 5701 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 5761 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5821 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5881 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5941 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 6001 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6061 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6181 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 6301 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 6361 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 6421 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 6481 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 6541 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 6601 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 6661 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 6721 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6781 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 6841 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6901 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6961 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 7021 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7081 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 7141 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7201 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7261 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 7321 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 7381 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 7441 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 7501 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc 7561 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag 7621 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag 7681 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt 7741 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc 7801 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 7861 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 7921 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 7981 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 8041 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 8101 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 8161 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 8221 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 8281 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg 8341 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 8401 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 8461 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 8521 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg 8581 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca 8641 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag 8701 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa 8761 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct 8821 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt 8881 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg 8941 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa 9001 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga 9061 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt 9121 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg 9181 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc 9241 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa 9301 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa 9361 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg 9421 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg 9481 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga 9541 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga 9601 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa 9661 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg 9721 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc 9781 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga 9841 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact 9901 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg 9961 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 10021 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca 10081 gcagagatcc agtttggtta gatctcgagt ttaccactcc ctatcagtga tagagaaaag 10141 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca 10201 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga 10261 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt 10321 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt 10381 gatagagaaa agtgaaagtc gagctcggta cccgggtcga //