LOCUS       Exported                7086 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain).
ACCESSION   .
VERSION     .
KEYWORDS    Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Rauch S, He C, Dickinson BC
  TITLE     Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation
            of Single RNAs.
  JOURNAL   J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 
            10.1021/jacs.8b05012. Epub 2018 Sep 18.
  PUBMED    30183280
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4364..4387
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4505..4523)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4560..4681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4571..4590)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4625..4644
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             5080..5874
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5134..5153)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5744..5763
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(6046..6634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6126..6145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    6750..7033
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggcagatca ctagaagctt acgctaccac catgaacatc cccgctctgg tggaaaacca
     1081 gaagaagtac tttggcacct acagcgtgat ggccatgctg aacgctcaga ccgtgctgga
     1141 ccacatccag aaggtggccg atattgaggg cgagcagaac gagaacaacg agaatctgtg
     1201 gtttcacccc gtgatgagcc acctgtacaa cgccaagaac ggctacgaca agcagcccga
     1261 gaaaaccatg ttcatcatcg agcggctgca gagctacttc ccattcctga agatcatggc
     1321 cgagaaccag agagagtaca gcaacggcaa gtacaagcag aaccgcgtgg aagtgaacag
     1381 caacgacatc ttcgaggtgc tgaagcgcgc cttcggcgtg ctgaagatgt acagggacct
     1441 gaccaacgca tacaagacct acgaggaaaa gctgaacgac ggctgcgagt tcctgaccag
     1501 cacagagcaa cctctgagcg gcatgatcaa caactactac acagtggccc tgcggaacat
     1561 gaacgagaga tacggctaca agacagagga cctggccttc atccaggaca agcggttcaa
     1621 gttcgtgaag gacgcctacg gcaagaaaaa gtcccaagtg aataccggat tcttcctgag
     1681 cctgcaggac tacaacggcg acacacagaa gaagctgcac ctgagcggag tgggaatcgc
     1741 cctgctgatc tgcctgttcc tggacaagca gtacatcaac atctttctga gcaggctgcc
     1801 catcttctcc agctacaatg cccagagcga ggaacggcgg atcatcatca gatccttcgg
     1861 catcaacagc atcaagctgc ccaaggaccg gatccacagc gagaagtcca acaagagcgt
     1921 ggccatggat atgctcaacg aagtgaagcg gtgccccgac gagctgttca caacactgtc
     1981 tgccgagaag cagtcccggt tcagaatcat cagcgacgac cacaatgaag tgctgatgaa
     2041 gcggagcagc gacagattcg tgcctctgct gctgcagtat atcgattacg gcaagctgtt
     2101 cgaccacatc aggttccacg tgaacatggg caagctgaga tacctgctga aggccgacaa
     2161 gacctgcatc gacggccaga ccagagtcag agtgatcgag cagcccctga acggcttcgg
     2221 cagactggaa gaggccgaga caatgcggaa gcaagagaac ggcaccttcg gcaacagcgg
     2281 catccggatc agagacttcg agaacatgaa gcgggacgac gccaatcctg ccaactatcc
     2341 ctacatcgtg gacacctaca cacactacat cctggaaaac aacaaggtcg agatgtttat
     2401 caacgacaaa gaggacagcg ccccactgct gcccgtgatc gaggatgata gatacgtggt
     2461 caagacaatc cccagctgcc ggatgagcac cctggaaatt ccagccatgg ccttccacat
     2521 gtttctgttc ggcagcaaga aaaccgagaa gctgatcgtg gacgtgcaca accggtacaa
     2581 gagactgttc caggccatgc agaaagaaga agtgaccgcc gagaatatcg ccagcttcgg
     2641 aatcgccgag agcgacctgc ctcagaagat cctggatctg atcagcggca atgcccacgg
     2701 caaggatgtg gacgccttca tcagactgac cgtggacgac atgctgaccg acaccgagcg
     2761 gagaatcaag agattcaagg acgaccggaa gtccattcgg agcgccgaca acaagatggg
     2821 aaagagaggc ttcaagcaga tctccacagg caagctggcc gacttcctgg ccaaggacat
     2881 cgtgctgttt cagcccagcg tgaacgatgg cgagaacaag atcaccggcc tgaactaccg
     2941 gatcatgcag agcgccattg ccgtgtacga tagcggcgac gattacgagg ccaagcagca
     3001 gttcaagctg atgttcgaga aggcccggct gatcggcaag ggcacaacag agcctcatcc
     3061 atttctgtac aaggtgttcg cccgcagcat ccccgccaat gccgtcgagt tctacgagcg
     3121 ctacctgatc gagcggaagt tctacctgac cggcctgtcc aacgagatca agaaaggcaa
     3181 cagagtggat gtgcccttca tccggcggga ccagaacaag tggaaaacac ccgccatgaa
     3241 gaccctgggc agaatctaca gcgaggatct gcccgtggaa ctgcccagac agatgttcga
     3301 caatgagatc aagtcccacc tgaagtccct gccacagatg gaaggcatcg acttcaacaa
     3361 tgccaacgtg acctatctga tcgccgagta catgaagaga gtgctggacg acgacttcca
     3421 gaccttctac cagtggaacc gcaactaccg gtacatggac atgcttaagg gcgagtacga
     3481 cagaaagggc tccctgcagc actgcttcac cagcgtggaa gagagagaag gcctctggaa
     3541 agagcgggcc tccagaacag agcggtacag aaagcaggcc agcaacaaga tccgcagcaa
     3601 ccggcagatg agaaacgcca gcagcgaaga gatcgagaca atcctggata agcggctgag
     3661 caacagccgg aacgagtacc agaaaagcga gaaagtgatc cggcgctaca gagtgcagga
     3721 tgccctgctg tttctgctgg ccaaaaagac cctgaccgaa ctggccgatt tcgacggcga
     3781 gaggttcaaa ctgaaagaaa tcatgcccga cgccgagaag ggaatcctga gcgagatcat
     3841 gcccatgagc ttcaccttcg agaaaggcgg caagaagtac accatcacca gcgagggcat
     3901 gaagctgaag aactacggcg acttctttgt gctggctagc gacaagagga tcggcaacct
     3961 gctggaactc gtgggcagcg acatcgtgtc caaagaggat ggcggtagtg gaggaagcgg
     4021 ggggagcgga gggagtggtg gatccggcgg ctccatgcac gatgctgttt ttgggcagcc
     4081 tgggggcctg gggaacaaca tctatcagca caggttcaat tttttccctg aaaaccctgc
     4141 gttctcagca tgggggacaa gtgggtctca aggtcagcag acccagagct ccgcgtatgg
     4201 gagcagctac acctaccccc cgagctccct gggtggcacg gtggttgatg ggcagccagg
     4261 ctttcacagc gacaccctca gcaaggcccc cgggatgaac agcctggagc agggcatggt
     4321 tggcctgaag attggggacg tcagctcctc cgccgtcgga tccgattaca aggacgacga
     4381 tgacaagtag tgtttaaacg aattcgggct cggtacacgg ggatcctcta gagtcgacct
     4441 gcaggcatgc aagctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     4501 gccaccgctg agcaataact agcataaccc cttggggcgg ccgcttcgag cagacatgat
     4561 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4621 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4681 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     4741 tttaagcaag taaaacctct acaaatgtgg taaaatcgaa ttctaatgga tcctctttgc
     4801 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     4861 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     4921 cttgcggcag cgtgagcttc aaaagaattg ccagctgggg cgccctctgg taaggttggg
     4981 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
     5041 tcaagatctg atcaagagac aggatgacgg tcgtttcgca tgcttgaaca agatggattg
     5101 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5161 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5221 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5281 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5341 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5401 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5461 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgcactcgg
     5521 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5581 gccgaactgt tcgccaggct caaggcgcgt atgccggatg gtgaggatct cgtcgtgact
     5641 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5701 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5761 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5821 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5881 ctctggggtt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc actcaaaggc
     5941 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6001 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6061 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6121 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6181 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6241 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6301 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6361 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6421 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6481 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6541 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6601 gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct tttctacggg
     6661 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6721 aaggatcttc acctagatcc ttttatagtc cggaaataca ggaacgcacg ctggatggcc
     6781 cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag tgggggtaat
     6841 gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc aggcgcgatt
     6901 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg
     6961 aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc
     7021 tcgtagccat cgagataaac cgcagtactc cggccacgat gcgtccggcg tagaggatcg
     7081 agatct
//
