LOCUS       Exported                 103 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  Optimized aTc inducible pTet promoter for Rhodococcus opacus 
            expressing mCherry on pAL5000/pMB1 backbone with kanamycin 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    pDD131
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 103)
  AUTHORS   DeLorenzo DM, Henson WR, Moon TS
  TITLE     Development of Chemical and Metabolite Sensors for Rhodococcus 
            opacus PD630.
  JOURNAL   ACS Synth Biol. 2017 Oct 20;6(10):1973-1978. doi: 
            10.1021/acssynbio.7b00192. Epub 2017 Aug 10.
  PUBMED    28745867
REFERENCE   2  (bases 1 to 103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    42..60
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tctgaccagg gaaaatagcc ctctgacctg gggatttgac ttccctatca gtgatagaga
       61 taatctggga gtccctatca gtgatagaga aggcggtatc gat
//
