LOCUS Exported 12247 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus. ACCESSION . VERSION . KEYWORDS Ai133 (TITL-ssAPEX2tm) targeting vector SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12247) AUTHORS Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA, Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT, Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, Waters J, Li L, Tasic B, Zeng H TITLE A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality. JOURNAL Cell. 2018 Jul 12;174(2):465-480.e22. doi: 10.1016/j.cell.2018.06.035. PUBMED 30007418 REFERENCE 2 (bases 1 to 12247) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12247 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 364..386 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 378..395 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 3009..3323 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 3017..3035 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3053..3071 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3088..3106 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3124..3142 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3160..3178 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3195..3213 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3231..3249 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 3296..3320 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 3333..3366 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 3443..3491 /note="synthetic polyadenylation signal" polyA_signal 3500..3976 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3699..3718) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" polyA_signal 3988..4449 /label=PGK poly(A) signal /note="mouse phosphoglycerate kinase 1 polyadenylation signal" protein_bind 4476..4509 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 4592..4615 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 5369..5395 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" misc_feature 5495..6083 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5548..5568) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5966..5977) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6108..6125) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6114..6321 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" protein_bind 8819..8852 /label=attB /bound_moiety="phage phi-C31 integrase" /note="minimal attB site for the phi-C31 integrase (Groth et al., 2000)" promoter 8879..9378 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(8914..8937) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 9311..9330 /label=mPGK-F /note="Mouse PGK promoter, forward primer" primer_bind complement(10026..10042) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10026..10042) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10039..10061) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10050..10066 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10074..10104) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10119..10140 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10257..10274) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10428..11016) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10508..10527) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11187..12047) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11810..11829 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12048..12152) /gene="bla" /label=AmpR promoter primer_bind 12220..12238 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc 421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgttttat 481 gccgcggtta ccgtgaagat atcttaatta agaattcgag ctcgctagag ccccatcctc 541 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt 601 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc 661 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac 721 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag 781 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc 841 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc 901 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt 961 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat 1021 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa 1081 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc 1141 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc 1201 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat 1261 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg 1321 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc 1381 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc 1441 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa 1501 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag 1561 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc 1621 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct 1681 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagcg 1741 agctcggtac ccagagcccc atcctcactg actccgtcct ggagttggat gagagataat 1801 ggccttacgt tgtgccaggg gagggtcggg ctggatttag caagatttac cttctccaaa 1861 gagcggtgct gcagtggcac agctgcccac ggaggtgggg gggtcaccgt ccctggaggt 1921 gatgaagaac tgtggggatg tggcactgag ggacatggcc agtgggcacg gtgggtgggt 1981 tggggttggt cttggggatc ttggagggct tttccagcct tcatgatttg acgattgtat 2041 gaacatctac atggcaattc tccagctgcc tgtcccagtc ctactgaccc agctgtatct 2101 ctccaggcaa gctcttccac cccttctgct tgcatccaga caccatcaaa catgcaggct 2161 cagacacagg gaccagcagt gtctgtggcc tttttgtgct cctctccatg ctgggtttta 2221 acttgctctt tgtccttcta tcctatcttc ttatccttaa ggctgttctg aacgctgtga 2281 cttggagagt gtcccagagc cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac 2341 ttccttatcc ccaagatctg cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc 2401 cagaccaaca atgtcacagc aaactccccc ttgataggac aagggggaat ggctttacac 2461 tgagacaggg gaggtttggg ttggatatga ggaggcagtt tttcccccag agggtggtga 2521 cgcactgaac aggttgccca aggaggctgt ggatgcccca tccctgcagg cattcaaggc 2581 caggctggat gtggctctgg gcagcctggg ctgctggttg atgaccctgc acatagcagg 2641 gggttggatc tggatgagca ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc 2701 attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg 2761 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc 2821 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag 2881 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc 2941 tttggggggg ggctgtccct ctaggggtac ccggggatcc accggtatcg attacctatc 3001 acaaattcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc 3061 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt 3121 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac 3181 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag 3241 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc 3301 gtttagtgaa ccgtcagatc gcctatttaa atataacttc gtatagcata cattatacga 3361 agttattcgc gatgaataaa tgaaagcttg cagatcccct cagcaccatg gctagcggca 3421 gcctcggagt ttgaatagat agaataaaat atctttattt tcattccatc tgtgtgttgg 3481 ttttttgtgt gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc 3541 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat 3601 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa 3661 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag 3721 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct 3781 gcctcaggct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt 3841 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc 3901 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc 3961 tcccttccct gtccttctgc ctcagcgctg taagtctgca gaaattgatg atctattaaa 4021 caataaagat gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag 4081 aacagagtac ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg 4141 attagataaa tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc 4201 atagttggat atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc 4261 actcatgatc tatagatcta tagatctctc gtgggatcat tgtttttctc ttgattccca 4321 ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt ttcatagcct gaagaacgag 4381 atcagcagcc tctgttccac atacacttca ttctcagtat tgttttgcca agttctaatt 4441 ccatcagaaa gcttttaatt aactgaatag ctaacataac ttcgtatagc atacattata 4501 cgaagttatc acgcgtgcca ccatgaaatg taaattcctg ggtagcttct ttctgctctt 4561 cagcctttcc ggcaaagggg cggactgcat ggactataaa gacgacgacg ataaaggaaa 4621 gagctaccca acagtgtccg ctgactatca ggatgctgtc gaaaaagcaa agaaaaagct 4681 gagggggttt atcgctgaga agagatgcgc accactgatg ctccggctgg ccttccactc 4741 cgctggtacc tttgacaaag gcaccaagac aggcggaccc ttcggaacca tcaagcaccc 4801 tgcagaactc gcccattctg ctaacaatgg cctggacatc gccgtgagac tgctcgaacc 4861 tctcaaggcc gagttcccaa ttctgagtta cgctgacttt tatcagctgg caggcgtggt 4921 cgccgtggag gtcactgggg gtcctaaagt gccattccac ccaggacgag aggacaagcc 4981 tgaaccacct ccagagggac gactgccaga tcccaccaaa gggagcgacc atctgaggga 5041 cgtgttcggc aaggcaatgg gcctcactga ccaggacatc gtggccctgt ccggcggaca 5101 caccattgga gccgctcata aggaaagaag tggattcgag gggccctgga ccagcaaccc 5161 cctgatcttc gacaattcct actttactga gctgctctct ggagagaaag aagggctgct 5221 ccagctgcca tcagacaagg ctctgctcag cgaccccgtg ttcaggccac tggtcgacaa 5281 atacgcagcc gacgaagatg cattctttgc cgattatgct gaagcacatc agaagctctc 5341 tgagctggga ttcgcagacg ctctccagct gccacctctg gagcgcctca ccctggatgt 5401 cacagtgggg gtcggtgcag gaggactcct cttggtgctc ttggtggcgc tttttatttt 5461 ctgtatctga acgcgttacg taaagcttgt cgataatcaa cctctggatt acaaaatttg 5521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 5581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 5641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 5701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 5761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 5821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5881 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg 5941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6001 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 6061 ctccctttgg gccgcctccc cgcatcgaca ccgagcgctg ctcgaggcct cgactgtgcc 6121 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 6181 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 6241 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 6301 caatagcagg catgctgggg acacgtggga tcctctagag ccccatcctc actgactccg 6361 tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat 6421 ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt 6481 gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat 6541 ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca 6601 gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc 6661 agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc 6721 cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg 6781 tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc 6841 ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg 6901 tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac 6961 tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata 7021 ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc 7081 agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc 7141 cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg 7201 gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa 7261 cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc 7321 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga 7381 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg 7441 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag 7501 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag ccccatcctc 7561 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt 7621 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc 7681 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac 7741 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag 7801 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc 7861 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc 7921 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt 7981 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat 8041 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa 8101 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc 8161 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc 8221 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat 8281 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg 8341 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc 8401 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc 8461 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa 8521 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag 8581 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc 8641 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct 8701 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag 8761 tcgacggcgc gccgtaaaag cttggcatag atcttattgc agaagccata tgttttaggt 8821 gccagggcgt gcccttgggc tccccgggcg cgtttgggcg gccgcatcga attctaccgg 8881 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc 8941 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc 9001 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg 9061 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt 9121 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg 9181 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt 9241 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc 9301 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc 9361 tcatctccgg gcctttcgac ctgcaatcgc cgctagccgc caccatgaaa aagcctgaac 9421 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga 9481 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat 9541 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc 9601 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga 9661 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa 9721 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg 9781 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta 9841 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga 9901 tggacgacac cgtcagtgta agtgctagcc cctaggcctg aatcgaagtt cctattcttc 9961 aaaaggtata ggaacttcga gctcgtcgac tgcagaggcc tgcatgcaag cttggcgtaa 10021 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 10081 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 10141 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 10201 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 10261 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 10321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 10381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 10441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 10501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 10561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 10621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 10681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 10741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 10801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 10861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 10921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 10981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 11041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 11101 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 11161 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 11221 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 11281 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 11341 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 11401 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 11461 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 11521 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 11581 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 11641 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 11701 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 11761 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 11821 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 11881 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 11941 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 12001 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 12061 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 12121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 12181 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 12241 tttcgtc //