LOCUS       Exported               16543 bp ds-DNA     circular SYN 11-APR-2024
DEFINITION  Target a Cre-dependent GFP and a tTA2 expression cassette into the 
            mouse TIGRE locus.
ACCESSION   .
VERSION     .
KEYWORDS    Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16543)
  AUTHORS   Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, 
            Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts 
            LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz 
            GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA,
            Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT,
            Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, 
            Waters J, Li L, Tasic B, Zeng H
  TITLE     A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced 
            Brain-Cell-Type Targeting and Functionality.
  JOURNAL   Cell. 2018 Jul 12;174(2):465-480.e22. doi: 
            10.1016/j.cell.2018.06.035.
  PUBMED    30007418
REFERENCE   2  (bases 1 to 16543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 11, 2024 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16543
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2975..3056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3000..3019
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    3070..3340
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3373..3411
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3408..3432
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3545..3578
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    3655..3703
                     /note="synthetic polyadenylation signal"
     polyA_signal    3712..4188
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    4200..4661
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    4688..4721
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      4735..4744
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4741..5460
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4741..5457
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4786..4807)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5047..5066)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5394..5415
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5485..6073
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5956..5967)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6104..6311
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        8835..9138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9140..9417
                     /label=chicken beta-actin promoter
     intron          9418..10434
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10358..10380
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10442..10461
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(10522..10555)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     polyA_signal    10593..10641
                     /note="synthetic polyadenylation signal"
     polyA_signal    10650..11126
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(11161..11180)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11205..11253
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(11432..11465)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     CDS             11486..12232
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    12275..12863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(12746..12757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    12894..13101
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    13115..13148
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        13175..13674
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13210..13233)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     13607..13626
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(14322..14338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14322..14338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14335..14357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14346..14362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(14370..14400)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14415..14436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14553..14570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14724..15312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14804..14823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15483..16343)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16106..16125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16344..16448)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     16516..16534
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgtttaat
      481 tcgagctcgc tagagcccca tcctcactga ctccgtcctg gagttggatg agagataatg
      541 gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc ttctccaaag
      601 agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc cctggaggtg
      661 atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg tgggtgggtt
      721 ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga cgattgtatg
      781 aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca gctgtatctc
      841 tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac atgcaggctc
      901 agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc tgggttttaa
      961 cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga acgctgtgac
     1021 ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct gtcctgcact
     1081 tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat gtgtcacccc
     1141 agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg gctttacact
     1201 gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga gggtggtgac
     1261 gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc attcaaggcc
     1321 aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca catagcaggg
     1381 ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat gattctgtca
     1441 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     1501 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     1561 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     1621 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     1681 ttgggggggg gctgtccctc tagcgagctc ggtacccaga gccccatcct cactgactcc
     1741 gtcctggagt tggatgagag ataatggcct tacgttgtgc caggggaggg tcgggctgga
     1801 tttagcaaga tttaccttct ccaaagagcg gtgctgcagt ggcacagctg cccacggagg
     1861 tgggggggtc accgtccctg gaggtgatga agaactgtgg ggatgtggca ctgagggaca
     1921 tggccagtgg gcacggtggg tgggttgggg ttggtcttgg ggatcttgga gggcttttcc
     1981 agccttcatg atttgacgat tgtatgaaca tctacatggc aattctccag ctgcctgtcc
     2041 cagtcctact gacccagctg tatctctcca ggcaagctct tccacccctt ctgcttgcat
     2101 ccagacacca tcaaacatgc aggctcagac acagggacca gcagtgtctg tggccttttt
     2161 gtgctcctct ccatgctggg ttttaacttg ctctttgtcc ttctatccta tcttcttatc
     2221 cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc agagccctca acacctgcat
     2281 gtcccacgtc catgctgtcc tgcacttcct tatccccaag atctgcctct ccgtgatgca
     2341 ctgaattggc aaacatgtgt caccccagac caacaatgtc acagcaaact cccccttgat
     2401 aggacaaggg ggaatggctt tacactgaga caggggaggt ttgggttgga tatgaggagg
     2461 cagtttttcc cccagagggt ggtgacgcac tgaacaggtt gcccaaggag gctgtggatg
     2521 ccccatccct gcaggcattc aaggccaggc tggatgtggc tctgggcagc ctgggctgct
     2581 ggttgatgac cctgcacata gcagggggtt ggatctggat gagcactgtg ctcctttgca
     2641 acccaggccg ttctatgatt ctgtcattct aaatctctct ttcagcctaa agctttttcc
     2701 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     2761 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     2821 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     2881 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctctagg ggtacccggg
     2941 gatccaccgg tatcgattcg gccggccgac gtcggcagtg aaaaaaatgc tttatttgtg
     3001 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagtttcga
     3061 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     3121 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     3181 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     3241 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     3301 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     3361 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     3421 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     3481 gaccgatcca gcctccgcgg ccccgaattc gagctcggta cccggggatc aaattcaatt
     3541 aaatataact tcgtatagca tacattatac gaagttattc gcgatgaata aatgaaagct
     3601 tgcagatccc ctcagcacca tggctagcgg cagcctcgga gtttgaatag atagaataaa
     3661 atatctttat tttcattcca tctgtgtgtt ggttttttgt gtgagatcta cgggtggcat
     3721 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     3781 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     3841 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     3901 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     3961 atctccgcct cctgggttca agcgattctc ctgcctcagg ctcccgagtt gttgggattc
     4021 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     4081 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     4141 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gcctcagcgc
     4201 tgtaagtctg cagaaattga tgatctatta aacaataaag atgtccacta aaatggaagt
     4261 ttttcctgtc atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag
     4321 gattggagct acgggggtgg gggtggggtg ggattagata aatgcctgct ctttactgaa
     4381 ggctctttac tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc
     4441 aaaaccaaat taagggccag ctcattcctc ccactcatga tctatagatc tatagatctc
     4501 tcgtgggatc attgtttttc tcttgattcc cactttgtgg ttctaagtac tgtggtttcc
     4561 aaatgtgtca gtttcatagc ctgaagaacg agatcagcag cctctgttcc acatacactt
     4621 cattctcagt attgttttgc caagttctaa ttccatcaga aagcttttaa ttaactgaat
     4681 agctaacata acttcgtata gcatacatta tacgaagtta tcacgcgtgc tagcgccacc
     4741 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4801 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4861 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4921 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4981 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5041 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5101 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5161 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     5221 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     5281 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5341 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     5401 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     5461 acgcgttacg taaagcttgt cgataatcaa cctctggatt acaaaatttg tgaaagattg
     5521 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5581 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5641 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5701 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5761 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5821 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5881 tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc
     5941 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6001 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6061 gccgcctccc cgcatcgaca ccgagcgctg ctcgaggcct cgactgtgcc ttctagttgc
     6121 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6181 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6241 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6301 catgctgggg acacgtggga tcctctagag ccccatcctc actgactccg tcctggagtt
     6361 ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat ttagcaagat
     6421 ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt gggggggtca
     6481 ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat ggccagtggg
     6541 cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca gccttcatga
     6601 tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc agtcctactg
     6661 acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc cagacaccat
     6721 caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg tgctcctctc
     6781 catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc ttaaggctgt
     6841 tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg tcccacgtcc
     6901 atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac tgaattggca
     6961 aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata ggacaagggg
     7021 gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc agtttttccc
     7081 ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg
     7141 caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttgatgacc
     7201 ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa cccaggccgt
     7261 tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc
     7321 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     7381 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
     7441 ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt
     7501 acatccctgg gggctttggg ggggggctgt ccctctagag ccccatcctc actgactccg
     7561 tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat
     7621 ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt
     7681 gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat
     7741 ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca
     7801 gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc
     7861 agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc
     7921 cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg
     7981 tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc
     8041 ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg
     8101 tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac
     8161 tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata
     8221 ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc
     8281 agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc
     8341 cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg
     8401 gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa
     8461 cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc
     8521 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     8581 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     8641 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     8701 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag tcgacggcgc
     8761 gccctgcagg aatctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     8821 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     8881 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     8941 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     9001 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     9061 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9121 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     9181 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     9241 ggggcggggg gggggggggg gcgcgcgccr ggsggggsgg ggsggggsgr ggggsggggs
     9301 ggggsgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     9361 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     9421 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     9481 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     9541 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     9601 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     9661 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     9721 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     9781 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     9841 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     9901 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     9961 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
    10021 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
    10081 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
    10141 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
    10201 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
    10261 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
    10321 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
    10381 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
    10441 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgat ttgataccgc
    10501 gggccccgcg tggacggtac cataacttcg tataaagtat cctatacgaa gttatccatg
    10561 gctagcggca gcctcggagt ttgaatagat agaataaaat atctttattt tcattccatc
    10621 tgtgtgttgg ttttttgtgt gagatctacg ggtggcatcc ctgtgacccc tccccagtgc
    10681 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
    10741 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
    10801 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
    10861 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
    10921 cgattctcct gcctcaggct cccgagttgt tgggattcca ggcatgcatg accaggctca
    10981 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
    11041 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
    11101 gaaccactgc tcccttccct gtccttctgc ctcagctaat tgagtagggg ggaggctaac
    11161 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
    11221 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
    11281 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
    11341 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
    11401 cggcaggccg tggaagttcc tattctctag aataacttcg tataaagtat cctatacgaa
    11461 gttatacgcg tccatttaat tcaccatgtc tagactggac aagagcaaag tcataaactc
    11521 tgctctggaa ttactcaatg aagtcggtat cgaaggcctg acgacaagga aactcgctca
    11581 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
    11641 cgatgccctg gcaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
    11701 cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc
    11761 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
    11821 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
    11881 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggatc aggagcatca
    11941 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
    12001 agcaattgag ctgttcgacc atcagggagc cgaacctgcc ttccttttcg gcctggaact
    12061 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct
    12121 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
    12181 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa
    12241 ggatcaaatg gcgcgttacg taaagcttgt cgataatcaa cctctggatt acaaaatttg
    12301 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    12361 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    12421 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    12481 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    12541 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    12601 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    12661 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
    12721 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    12781 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    12841 ctccctttgg gccgcctccc cgcatcgaca ccgagcgctg ctcgaggcct cgactgtgcc
    12901 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
    12961 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
    13021 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
    13081 caatagcagg catgctgggg acacgtggga tccggtgcca gggcgtgccc ttgggctccc
    13141 cgggcgcgtt tgggcggccg catcgaattc taccgggtag gggaggcgct tttcccaagg
    13201 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
    13261 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
    13321 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
    13381 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
    13441 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
    13501 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
    13561 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
    13621 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
    13681 aatcgccgct agccgccacc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
    13741 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
    13801 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
    13861 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
    13921 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
    13981 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
    14041 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
    14101 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
    14161 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgtaagtg
    14221 ctagccccta ggcctgaatc gaagttccta ttcttcaaaa ggtataggaa cttcgagctc
    14281 gtcgactgca gaggcctgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
    14341 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    14401 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    14461 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    14521 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    14581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    14641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    14701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    14761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    14821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    14881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    14941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    15001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    15061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    15121 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    15181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    15241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    15301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    15361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    15421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    15481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    15541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    15601 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    15661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    15721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    15781 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    15841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    15901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    15961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    16021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    16081 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    16141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    16201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    16261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    16321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    16381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    16441 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    16501 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
