LOCUS Exported 4914 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules. ACCESSION . VERSION . KEYWORDS pSW610 - GR-LhG4_BD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4914) AUTHORS Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb T, Wolf S TITLE A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene Expression in Arabidopsis. JOURNAL Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463. PUBMED 30026289 REFERENCE 2 (bases 1 to 4914) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4914 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..28) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 96..200 /gene="bla" /label=AmpR promoter CDS 201..1061 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(419..438) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1232..1820 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1721..1740 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1974..1991 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2108..2129 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2144..2174 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2182..2198 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter 2212..2230 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 2212..2229 /label=SP6 /note="SP6 promoter, forward primer" CDS 4135..4479 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="activation domain of the GAL4 transcriptional activator" /label=GAL4 activation domain /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD EDTPPNPKKE" primer_bind complement(4500..4519) /label=T7 /note="T7 promoter, forward primer" promoter complement(4501..4519) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4529..4551) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(4745..4764) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4864..4886 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct 961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg 2221 acactataga agtgaagctt ggtctcaaac acaatggcta gtgaagctcg aaaaacaaag 2281 aaaaaaatca aagggattca gcaagccact gcaggagtct cacaagacac ttcggaaaat 2341 cctaacaaaa caatagttcc tgcagcatta ccacagctca cccctacctt ggtgtcactg 2401 ctggaggtga ttgaacccga ggtgttgtat gcaggatatg atagctctgt tccagattca 2461 gcatggagaa ttatgaccac actcaacatg ttaggtgggc gtcaagtgat tgcagcagtg 2521 aaatgggcaa aggcgatacc aggcttcaga aacttacacc tggatgacca aatgaccctg 2581 ctacagtact catggatgtt tctcatggca tttgccctgg gttggagatc atacagacaa 2641 tcaagtggaa acctgctctg ctttgctcct gatctgatta ttaatgagca gagaatgtct 2701 ctaccctgca tgtatgacca atgtaaacac atgctgtttg tctcctctga attacaaaga 2761 ttgcaggtat cctatgaaga gtatctctgt atgaaaacct tactgcttct ctcctcagtt 2821 cctaaggaag gtctgaagag ccaagagtta tttgatgaga ttcgaatgac ttatatcaaa 2881 gagctaggaa aagccatcgt caaaagggaa gggaactcca gtcagaactg gcaacggttt 2941 taccaactga caaagcttct ggactccatg catgaggtgg ttgagaatct ccttacctac 3001 tgcttccaga catttttgga taagaccatg agtattgaat tcccagagat gttagctgaa 3061 atcatcacta atcagatacc aaaatattca aatggaaata tcaaaaagct tctgtttcat 3121 caaaaatcta ctagcaaacc ggtaacgtta tacgacgtcg ctgaatacgc cggcgtttct 3181 catcaaaccg tttctagagt ggttaaccag gcttcacatg ttagcgctaa aacccgggaa 3241 aaagttgaag ctgccatggc tgagctcaac tacatcccga accgtgttgc gcagcagctg 3301 gctggtaaac aaagcttgct gatcggtgtc gcgacctcga gcttggccct gcacgcgccg 3361 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg 3421 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg 3481 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg 3541 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc 3601 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca 3661 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg 3721 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg 3781 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc 3841 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc 3901 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc 3961 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg 4021 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat 4081 cagctgttgc ccgtctcact ggtgaaaaga aaaaccacta gtggatcgga attcgccaat 4141 tttaatcaaa gtgggaatat tgctgatagc tcattgtcct tcactttcac taacagtagc 4201 aacggtccga acctcataac aactcaaaca aattctcaag cgctttcaca accaattgcc 4261 tcctctaacg ttcatgataa cttcatgaat aatgaaatca cggctagtaa aattgatgat 4321 ggtaataatt caaaaccact gtcacctggt tggacggacc aaactgcgta taacgcgttt 4381 ggaatcacta cagggatgtt taataccact acaatggatg atgtatataa ctatctattc 4441 gatgatgaag ataccccacc aaacccaaaa aaagagtaac tgctgagacc gaattctcgc 4501 cctatagtga gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 4561 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 4621 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc 4681 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc 4741 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg 4801 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 4861 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcga //