LOCUS Exported 4608 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION entry clone for NRF2. ACCESSION . VERSION . KEYWORDS pDONR223 NRF2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4608) AUTHORS Briggs KJ, Koivunen P, Cao S, Backus KM, Olenchock BA, Patel H, Zhang Q, Signoretti S, Gerfen GJ, Richardson AL, Witkiewicz AK, Cravatt BF, Clardy J, Kaelin WG Jr TITLE Paracrine Induction of HIF by Glutamate in Breast Cancer: EglN1 Senses Cysteine. JOURNAL Cell. 2016 Jun 30;166(1):126-39. doi: 10.1016/j.cell.2016.05.042. PUBMED 27368101 REFERENCE 2 (bases 1 to 4608) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4608 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 146..173 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 265..351 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 400..422 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 414..431 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 415..431 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 447..546 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2365..2464) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2481..2500) /label=T7 /note="T7 promoter, forward primer" promoter complement(2482..2500) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2505..2521) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2505..2521) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2952..3743 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3836..4424 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4325..4344 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4578..4595 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 61 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta 121 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt 181 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac 241 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca 301 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc 361 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa 421 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct 481 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 541 caggcttcat gatggacttg gagctgccgc cgccgggact cccgtcccag caggacatgg 601 atttgattga catactttgg aggcaagata tagatcttgg agtaagtcga gaagtatttg 661 acttcagtca gcgacggaaa gagtatgagc tggaaaaaca gaaaaaactt gaaaaggaaa 721 gacaagaaca actccaaaag gagcaagaga aagccttttt cgctcagtta caactagatg 781 aagagacagg tgaatttctc ccaattcagc cagcccagca catccagtca gaaaccagtg 841 gatctgccaa ctactcccag gttgcccaca ttcccaaatc agatgctttg tactttgatg 901 actgcatgca gcttttggcg cagacattcc cgtttgtaga tgacaatgag gtttcttcgg 961 ctacgtttca gtcacttgtt cctgatattc ccggtcacat cgagagccca gtcttcattg 1021 ctactaatca ggctcagtca cctgaaactt ctgttgctca ggtagcccct gttgatttag 1081 acggtatgca acaggacatt gagcaagttt gggaggagct attatccatt cctgagttac 1141 agtgtcttaa tattgaaaat gacaagctgg ttgagactac catggttcca agtccagaag 1201 ccaaactgac agaagttgac aattatcatt tttactcatc tataccctca atggaaaaag 1261 aagtaggtaa ctgtagtcca cattttctta atgcttttga ggattccttc agcagcatcc 1321 tctccacaga agaccccaac cagttgacag tgaactcatt aaattcagat gccacagtca 1381 acacagattt tggtgatgaa ttttattctg ctttcatagc tgagcccagt atcagcaaca 1441 gcatgccctc acctgctact ttaagccatt cactctctga acttctaaat gggcccattg 1501 atgtttctga tctatcactt tgcaaagctt tcaaccaaaa ccaccctgaa agcacagcag 1561 aattcaatga ttctgactcc ggcatttcac taaacacaag tcccagtgtg gcatcaccag 1621 aacactcagt ggaatcttcc agctatggag acacactact tggcctcagt gattctgaag 1681 tggaagagct agatagtgcc cctggaagtg tcaaacagaa tggtcctaaa acaccagtac 1741 attcttctgg ggatatggta caacccttgt caccatctca ggggcagagc actcacgtgc 1801 atgatgccca atgtgagaac acaccagaga aagaattgcc tgtaagtcct ggtcatcgga 1861 aaaccccatt cacaaaagac aaacattcaa gccgcttgga ggctcatctc acaagagatg 1921 aacttagggc aaaagctctc catatcccat tccctgtaga aaaaatcatt aacctccctg 1981 ttgttgactt caacgaaatg atgtccaaag agcagttcaa tgaagctcaa cttgcattaa 2041 ttcgggatat acgtaggagg ggtaagaata aagtggctgc tcagaattgc agaaaaagaa 2101 aactggaaaa tatagtagaa ctagagcaag atttagatca tttgaaagat gaaaaagaaa 2161 aattgctcaa agaaaaagga gaaaatgaca aaagccttca cctactgaaa aaacaactca 2221 gcaccttata tctcgaagtt ttcagcatgc tacgtgatga agatggaaaa ccttattctc 2281 ctagtgaata ctccctgcag caaacaagag atggcaatgt tttccttgtt cccaaaagta 2341 agaagccaga tgttaagaaa aacaacccag ctttcttgta caaagttggc attataagaa 2401 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta 2461 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct 2521 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat 2581 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg 2641 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct 2701 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct 2761 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt 2821 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc 2881 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa 2941 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag 3001 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg 3061 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa 3121 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc 3181 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca 3241 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca 3301 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa 3361 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg 3421 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc 3481 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg 3541 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc 3601 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag 3661 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga 3721 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga 3781 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 3841 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 3901 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 3961 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 4021 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 4081 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 4141 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 4201 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 4261 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 4321 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 4381 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 4441 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 4501 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 4561 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagc //