LOCUS Exported 5448 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element. ACCESSION . VERSION . KEYWORDS pAAV-mDlx-GFP-Fishell-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5448) AUTHORS Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G, Fishell G TITLE A viral strategy for targeting and manipulating interneurons across vertebrate species. JOURNAL Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430. PUBMED 27798629 REFERENCE 2 (bases 1 to 5448) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5448 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" intron 849..981 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 964..983 /label=pMT2-F /note="Synthetic intron, forward primer" regulatory 1024..1033 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1030..1749 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 1030..1746 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1075..1096) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1336..1355) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1683..1704 /label=EGFP-C /note="EGFP, forward primer" misc_feature 1794..2382 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(1847..1867) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2265..2276) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 2551..2632 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2588..2607) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2642..2661 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" repeat_region 2711..2851 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 2711..2840 /label=AAV2 ITR rep_origin 2926..3381 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3013..3032) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3223..3244 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3398..3417) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3517..3539 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3577..3595) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3663..3767 /gene="bla" /label=AmpR promoter CDS 3768..4628 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3986..4005) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4799..5387 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5288..5307 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtttaatt aagaattcta tacactcaca gtggtttggc 181 atatatttgg tgaaattttt taaggaaaaa ttagtgttgg tttcgatata tggtagcttt 241 ttctctaaca taatttgaat aattcagcaa agccctacta ccagctgtac ttctgcagcc 301 tcttccattc tttccagcat tataattttg gttaattttc aattttaggt cctacgtctc 361 tgcaatttgt gtatgaataa cagaataatt tccctctttt gtttcgcctt tcctgttcct 421 gaatctaaat aaagatggct ttttagtatt aaaagtggaa gaaaattaca ggtaattatc 481 tttgacggta aaaacgctgt aatcagcggg ctacatgaaa aattactcta attatggctg 541 catttaagag aatggaaaaa aaccttcttg tggataaaaa ccttaaattg tccccaatgt 601 ctgcttcaaa ttggatggca ctgcagctgg aggctttgtt cagaattgat cctggggagc 661 tacgaaccca aagtttcaca gtaggaagct tgatatcgaa ttcctgcagc ccgggctggg 721 cataaaagtc agggcagagc catctattgc ttacatttgc ttctagcctg caggtcgagg 781 agcgcagcct tccagaagca gagcgcggcg ccttaagctg cagaagttgg tcgtgaggca 841 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 901 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 961 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taagaaacta 1021 gtagccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 1081 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 1141 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 1201 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 1261 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 1321 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 1381 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 1441 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 1501 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 1561 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 1621 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 1681 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 1741 tacaagtaaa ggcgcgccac ccctgcaggg aattcgatat caagcttatc gataatcaac 1801 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 1861 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 1921 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 1981 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 2041 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 2101 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 2161 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg 2221 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 2281 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 2341 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc 2401 tcgagagatc tgtgatagcg gccatcaagc tggccgcgac tctagatcat aatcagccat 2461 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 2521 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 2581 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 2641 tgtggtttgt ccaaactcat caatgtatca gcttatcgat accgcatgca cgtgcggacc 2701 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg 2761 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca 2821 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg 2881 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg 2941 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 3001 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 3061 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 3121 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga 3181 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 3241 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga 3301 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 3361 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat 3421 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 3481 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 3541 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 3601 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 3661 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3721 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3781 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3841 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3901 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3961 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 4021 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 4081 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 4141 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 4201 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 4261 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 4321 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 4381 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 4441 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 4501 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4561 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4621 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 4681 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 4741 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4801 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4861 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4921 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 4981 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 5041 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 5101 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 5161 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 5221 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 5281 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 5341 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 5401 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt //