LOCUS Exported 9864 bp ds-DNA linear SYN 10-JAN-2024 DEFINITION psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.. ACCESSION . VERSION . KEYWORDS psPAX2-D64V SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9864) AUTHORS Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM TITLE Tracking genome engineering outcome at individual DNA breakpoints. JOURNAL Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. PUBMED 21743461 REFERENCE 2 (bases 1 to 9864) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 10, 2024 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9864 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 257..279 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 341..360 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 439..1941 /codon_start=1 /gene="gag" /product="gag protein from human immunodeficiency virus 1" /label=HIV-1 gag /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ" CDS 1734..4745 /codon_start=1 /gene="pol" /product="pol protein from human immunodeficiency virus 1" /label=HIV-1 pol /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ MAGDDCVASRQDED" misc_feature 4430..4547 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 5319..5552 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 5737..5781 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" CDS 6075..6101 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" primer_bind complement(6386..6405) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 6451..6506 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(6505..6524) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(6867..6883) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6867..6883) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6880..6902) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6891..6907 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6915..6945) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6960..6981 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7040..7235 /label=SV40 promoter /note="SV40 early promoter" rep_origin 7086..7221 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7148..7167 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 7241..7375 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7278..7297) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7332..7351 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7443..7460) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7614..8202) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7694..7713) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8373..9233) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8996..9015 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9234..9338) /gene="bla" /label=AmpR promoter enhancer 9369..9748 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc 61 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag 121 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc 181 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg 241 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct 301 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg 361 ctgtctcatc attttggcaa agaattcggg ccgcgttgac gcgcacggca agaggcgagg 421 ggcggcgact ggtgagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat 481 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata 541 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca 601 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa 661 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata 721 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaaaaaa 781 gcacagcaag cagcagctga cacaggacac agcaatcagg tcagccaaaa ttaccctata 841 gtgcagaaca tccaggggca aatggtacat caggccatat cacctagaac tttaaatgca 901 tgggtaaaag tagtagaaga gaaggctttc agcccagaag tgatacccat gttttcagca 961 ttatcagaag gagccacccc acaagattta aacaccatgc taaacacagt ggggggacat 1021 caagcagcca tgcaaatgtt aaaagagacc atcaatgagg aagctgcaga atgggataga 1081 gtgcatccag tgcatgcagg gcctattgca ccaggccaga tgagagaacc aaggggaagt 1141 gacatagcag gaactactag tacccttcag gaacaaatag gatggatgac acataatcca 1201 cctatcccag taggagaaat ctataaaaga tggataatcc tgggattaaa taaaatagta 1261 agaatgtata gccctaccag cattctggac ataagacaag gaccaaagga accctttaga 1321 gactatgtag accgattcta taaaactcta agagccgagc aagcttcaca agaggtaaaa 1381 aattggatga cagaaacctt gttggtccaa aatgcgaacc cagattgtaa gactatttta 1441 aaagcattgg gaccaggagc gacactagaa gaaatgatga cagcatgtca gggagtgggg 1501 ggacccggcc ataaagcaag agttttggct gaagcaatga gccaagtaac aaatccagct 1561 accataatga tacagaaagg caattttagg aaccaaagaa agactgttaa gtgtttcaat 1621 tgtggcaaag aagggcacat agccaaaaat tgcagggccc ctaggaaaaa gggctgttgg 1681 aaatgtggaa aggaaggaca ccaaatgaaa gattgtactg agagacaggc taatttttta 1741 gggaagatct ggccttccca caagggaagg ccagggaatt ttcttcagag cagaccagag 1801 ccaacagccc caccagaaga gagcttcagg tttggggaag agacaacaac tccctctcag 1861 aagcaggagc cgatagacaa ggaactgtat cctttagctt ccctcagatc actctttggc 1921 agcgacccct cgtcacaata aagatagggg ggcaattaaa ggaagctcta ttagatacag 1981 gagcagatga tacagtatta gaagaaatga atttgccagg aagatggaaa ccaaaaatga 2041 tagggggaat tggaggtttt atcaaagtaa gacagtatga tcagatactc atagaaatct 2101 gcggacataa agctataggt acagtattag taggacctac acctgtcaac ataattggaa 2161 gaaatctgtt gactcagatt ggctgcactt taaattttcc cattagtcct attgagactg 2221 taccagtaaa attaaagcca ggaatggatg gcccaaaagt taaacaatgg ccattgacag 2281 aagaaaaaat aaaagcatta gtagaaattt gtacagaaat ggaaaaggaa ggaaaaattt 2341 caaaaattgg gcctgaaaat ccatacaata ctccagtatt tgccataaag aaaaaagaca 2401 gtactaaatg gagaaaatta gtagatttca gagaacttaa taagagaact caagatttct 2461 gggaagttca attaggaata ccacatcctg cagggttaaa acagaaaaaa tcagtaacag 2521 tactggatgt gggcgatgca tatttttcag ttcccttaga taaagacttc aggaagtata 2581 ctgcatttac catacctagt ataaacaatg agacaccagg gattagatat cagtacaatg 2641 tgcttccaca gggatggaaa ggatcaccag caatattcca gtgtagcatg acaaaaatct 2701 tagagccttt tagaaaacaa aatccagaca tagtcatcta tcaatacatg gatgatttgt 2761 atgtaggatc tgacttagaa atagggcagc atagaacaaa aatagaggaa ctgagacaac 2821 atctgttgag gtggggattt accacaccag acaaaaaaca tcagaaagaa cctccattcc 2881 tttggatggg ttatgaactc catcctgata aatggacagt acagcctata gtgctgccag 2941 aaaaggacag ctggactgtc aatgacatac agaaattagt gggaaaattg aattgggcaa 3001 gtcagattta tgcagggatt aaagtaaggc aattatgtaa acttcttagg ggaaccaaag 3061 cactaacaga agtagtacca ctaacagaag aagcagagct agaactggca gaaaacaggg 3121 agattctaaa agaaccggta catggagtgt attatgaccc atcaaaagac ttaatagcag 3181 aaatacagaa gcaggggcaa ggccaatgga catatcaaat ttatcaagag ccatttaaaa 3241 atctgaaaac aggaaagtat gcaagaatga agggtgccca cactaatgat gtgaaacaat 3301 taacagaggc agtacaaaaa atagccacag aaagcatagt aatatgggga aagactccta 3361 aatttaaatt acccatacaa aaggaaacat gggaagcatg gtggacagag tattggcaag 3421 ccacctggat tcctgagtgg gagtttgtca atacccctcc cttagtgaag ttatggtacc 3481 agttagagaa agaacccata ataggagcag aaactttcta tgtagatggg gcagccaata 3541 gggaaactaa attaggaaaa gcaggatatg taactgacag aggaagacaa aaagttgtcc 3601 ccctaacgga cacaacaaat cagaagactg agttacaagc aattcatcta gctttgcagg 3661 attcgggatt agaagtaaac atagtgacag actcacaata tgcattggga atcattcaag 3721 cacaaccaga taagagtgaa tcagagttag tcagtcaaat aatagagcag ttaataaaaa 3781 aggaaaaagt ctacctggca tgggtaccag cacacaaagg aattggagga aatgaacaag 3841 tagataaatt ggtcagtgct ggaatcagga aagtactatt tttagatgga atagataagg 3901 cccaagaaga acatgagaaa tatcacagta attggagagc aatggctagt gattttaacc 3961 taccacctgt agtagcaaaa gaaatagtag ccagctgtga taaatgtcag ctaaaagggg 4021 aagccatgca tggacaagta gactgtagcc caggaatatg gcagctagtt tgtacacatt 4081 tagaaggaaa agttatcttg gtagcagttc atgtagccag tggatatata gaagcagaag 4141 taattccagc agagacaggg caagaaacag catacttcct cttaaaatta gcaggaagat 4201 ggccagtaaa aacagtacat acagacaatg gcagcaattt caccagtact acagttaagg 4261 ccgcctgttg gtgggcgggg atcaagcagg aatttggcat tccctacaat ccccaaagtc 4321 aaggagtaat agaatctatg aataaagaat taaagaaaat tataggacag gtaagagatc 4381 aggctgaaca tcttaagaca gcagtacaaa tggcagtatt catccacaat tttaaaagaa 4441 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 4501 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca 4561 gggacagcag agatccagtt tggaaaggac cagcaaagct cctctggaaa ggtgaagggg 4621 cagtagtaat acaagataat agtgacataa aagtagtgcc aagaagaaaa gcaaagatca 4681 tcagggatta tggaaaacag atggcaggtg atgattgtgt ggcaagtaga caggatgagg 4741 attaacacat ggaattctgc aacaactgct gtttatccat ttcagaattg ggtgtcgaca 4801 tagcagaata ggcgttactc gacagaggag agcaagaaat ggagccagta gatcctagac 4861 tagagccctg gaagcatcca ggaagtcagc ctaaaactgc ttgtaccaat tgctattgta 4921 aaaagtgttg ctttcattgc caagtttgtt tcatgacaaa agccttaggc atctcctatg 4981 gcaggaagaa gcggagacag cgacgaagag ctcatcagaa cagtcagact catcaagctt 5041 ctctatcaaa gcagtaagta gtacatgtaa tgcaacctat aatagtagca atagtagcat 5101 tagtagtagc aataataata gcaatagttg tgtggtccat agtaatcata gaatatagga 5161 aaatggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa 5221 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag 5281 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc 5341 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga 5401 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa 5461 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct 5521 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc 5581 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt 5641 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata 5701 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa 5761 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata 5821 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt 5881 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca 5941 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga 6001 gacagatcca ttcgattagt gaacggatcc ttggcactta tctgggacga tctgcggagc 6061 ctgtgcctct tcagctacca ccgcttgaga gacttactct tgattgtaac gaggattgtg 6121 gaacttctgg gacgcagggg gtgggaagcc ctcaaatatt ggtggaatct cctacaatat 6181 tggagtcagg agctaaagaa tagtgctgtt agcttgctca atgccacagc catagcagta 6241 gctgagggga cagatagggt tatagaagta gtacaaggag cttgtagagc tattcgccac 6301 atacctagaa gaataagaca gggcttggaa aggattttgc tataagctcg aaacaaccgg 6361 tacctctaga actatagcta gcagatcttt ttccctctgc caaaaattat ggggacatca 6421 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag 6481 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa 6541 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca 6601 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt 6661 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt 6721 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc 6781 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc 6841 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 6901 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 6961 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 7021 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc 7081 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 7141 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 7201 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta 7261 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 7321 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg 7381 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 7441 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7501 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7561 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7621 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7681 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7741 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7801 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7861 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7921 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7981 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8041 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8101 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8161 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8221 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8281 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8341 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8401 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8461 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8521 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8581 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8641 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 8701 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 8761 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8821 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8881 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8941 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 9001 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 9061 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 9121 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 9181 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 9241 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 9301 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 9361 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 9421 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 9481 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 9541 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 9601 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 9661 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 9721 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct 9781 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 9841 tgcagcgatg ggggcggggg gggg //