LOCUS Exported 789 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS Axin GID 5-6/pCS2MT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 789) AUTHORS Hedgepeth CM, Deardorff MA, Rankin K, Klein PS TITLE Regulation of glycogen synthase kinase 3beta and downstream Wnt signaling by axin. JOURNAL Mol Cell Biol. 1999 Oct . 19(10):7147-57. PUBMED 10490650 REFERENCE 2 (bases 1 to 789) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..789 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 35..64 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 74..103 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 113..142 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 152..181 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 191..220 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" promoter complement(347..364) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 369..503 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(393..412) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 447..466 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(616..636) /label=T3 /note="T3 promoter, forward primer" promoter complement(616..634) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(655..671) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(655..671) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(668..690) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 679..695 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(703..733) /label=lac promoter /note="promoter for the E. coli lac operon" ORIGIN 1 cctgtctttt gcggacccat cgatttaagc tatggagcaa aagctcattt ctgaagagga 61 cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa 121 gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt 181 gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg agagcttggg 241 cgacctcacc atggagattc acgtcgatcc tgaaaaattt gctgctgagc ttataagtcg 301 cttggagggg gtcctgagag accgtgaagc actcgagcct ctagaactat agtgagtcgt 361 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa 421 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca 481 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc 541 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt 601 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata 661 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag 721 cataaagtgt aaagcctggg gtgcctaatg agtgagctac ctcacattaa ttgcgttgcg 781 ctcactgcc //