LOCUS Exported 4493 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION This plasmid expresses the full length human SUMO1.. ACCESSION . VERSION . KEYWORDS hSUMO1-HIS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4493) AUTHORS Hietakangas V, Ahlskog JK, Jakobsson AM, Hellesuo M, Sahlberg NM, Holmberg CI, Mikhailov A, Palvimo JJ, Pirkkala L, Sistonen L TITLE Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1. JOURNAL Mol Cell Biol. 2003 Apr . 23(8):2953-68. PUBMED 12665592 REFERENCE 2 (bases 1 to 4493) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4493 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..18 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" polyA_signal 419..540 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(456..475) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 510..529 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(574..591) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(745..1333) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(825..844) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1504..2364) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2127..2146 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2365..2469) /gene="bla" /label=AmpR promoter primer_bind 2537..2555 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(2593..2615) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 2715..2734 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin complement(2801..3256) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2938..2959) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3150..3169 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3382..3404 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3396..3413 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3397..3413 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3450..3470) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3558..3768 /label=SV40 promoter /note="SV40 early promoter" promoter 3580..3776 /label=SV40 promoter /note="SV40 early promoter" rep_origin 3627..3762 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3689..3708 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 3807..4379 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" primer_bind 4387..4406 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind 4434..4453 /label=T7 /note="T7 promoter, forward primer" promoter 4434..4452 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 4464..4475 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" ORIGIN 1 catcaccatc accatcacgg atccccggga atttccggtg gtggtggtgg aattctagac 61 tccatggctg accaggaggc aaaaccttca actgaggact tgggggataa gaaggaaggt 121 gaatatatta aactcaaagt cattggacag gatagcagtg agattcactt caaagtgaaa 181 atgacaacac atctcaagaa actcaaagaa tcatactgtc aaagacaggg tgttccaatg 241 aattcactca ggtttctctt tgagggtcag agaattgctg ataatcatac tccaaaagaa 301 ctgggaatgg aggaagaaga tgtgattgaa gtttatcagg aacaaacggg gggtcattca 361 acagtttaga tattgatcca tgcaatcacg aattcggatc cagatcttat taaagcagaa 421 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 481 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 541 tcatgtctgg tcgactctag actcttccgc ttcctcgctc actgactcgc tgcgctcggt 601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 961 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 1021 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 1081 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 1141 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 1201 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 1261 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 1321 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 1381 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 1441 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 1501 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 1561 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 1621 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 1681 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 1741 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 1801 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 1861 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 1921 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 1981 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 2041 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 2101 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 2161 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 2221 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 2281 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 2341 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 2401 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 2461 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 2521 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 2581 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 2641 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 2701 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 2761 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt 2821 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 2881 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 2941 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 3001 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 3061 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 3121 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 3181 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 3241 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 3301 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 3361 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt 3421 cgacttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 3481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaggaa 3541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 3661 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 3721 ctgagctatt ccagaagtag tgaagaggct tttttggagg cctaggcttt tgcaaaaagc 3781 tccggatcga tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt 3841 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa 3901 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc 3961 tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt 4021 taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt 4081 gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt 4141 cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata 4201 taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacatc ctggtcatca 4261 tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact 4321 ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttcttt ttcctacagc 4381 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgtaatacg 4441 actcactata gggcgaattc attaaagagg agaaattaac tatgagagga tcg //