LOCUS Exported 9120 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Tet-O-FUW-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9120) AUTHORS Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M TITLE Direct conversion of fibroblasts to functional neurons by defined factors. JOURNAL Nature. 2010 Feb 25. 463(7284):1035-41. PUBMED 20107439 REFERENCE 2 (bases 1 to 9120) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9120 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(46..65) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 237..616 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 618..816 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 771..791 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 834..1014 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1061..1186 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1679..1912 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2097..2141 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2439..2556 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2613..2883 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2916..2954 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2951..2975 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 3055..3064 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3061..3780 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3061..3777 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3106..3127) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3367..3386) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3714..3735 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3811..4399 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3864..3884) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4282..4293) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4402..4418) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4403..4419) /label=pBluescriptKS /note="For pBluescript vector" LTR 4924..5104 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5130..5147) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5136..5360 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5406..5834 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5493..5512) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5703..5724 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5843..5863) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5848..6177 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6028..6163 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6090..6109 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6225..6272 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6291..6665 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 6795..6916 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6832..6851) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6886..6905 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6965..6981) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6965..6981) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6978..7000) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6989..7005 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7013..7043) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7058..7079 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7196..7213) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7367..7955) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7447..7466) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8126..8986) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8749..8768 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8987..9091) /gene="bla" /label=AmpR promoter ORIGIN 1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc 841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg 1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat 1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg 1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa 1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct 1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac 1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca 1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa 1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga 1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag 1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag 1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga 1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc 1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc 1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt 1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta 1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta 2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga 2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa 2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa 2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat 2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag 2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg 2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt 2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa 2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga 2581 gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag aaaagtgaaa 2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc 2701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa 2761 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc 2821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag 2881 agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct 2941 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt 3001 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttccgccacc 3061 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 3121 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 3181 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 3241 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 3301 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 3361 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 3421 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 3481 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 3541 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 3601 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 3661 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 3721 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa 3781 acgcgtgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa 3841 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 3901 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 3961 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 4021 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 4081 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 4141 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 4201 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 4261 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 4321 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 4381 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca 4441 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag 4501 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag 4561 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 4621 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct 4681 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc 4741 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc 4801 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag 4861 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt 4921 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 4981 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 5041 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 5101 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 5161 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 5221 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 5281 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 5341 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 5401 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 5461 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 5521 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 5581 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 5641 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 5701 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 5761 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 5821 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 5881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 5941 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 6001 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 6061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 6121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 6181 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat 6241 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt 6301 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga 6361 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg 6421 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg 6481 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca 6541 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc 6601 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg 6661 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 6721 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 6781 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 6841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 6901 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 6961 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 7021 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 7081 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 7141 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 7201 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7261 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7321 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7381 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7441 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7501 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 7561 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 7621 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 7681 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7741 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7801 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7861 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7921 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7981 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 8041 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 8101 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 8161 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 8221 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 8281 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 8341 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 8401 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 8461 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 8521 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 8581 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 8641 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 8701 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 8761 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8821 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8881 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8941 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 9001 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 9061 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg //