LOCUS Exported 5172 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBabe HcRed SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5172) AUTHORS Boehm JS, Hession MT, Bulmer SE, Hahn WC TITLE Transformation of human and murine fibroblasts without viral oncoproteins. JOURNAL Mol Cell Biol. 2005 Aug . 25(15):6464-74. PUBMED 16024784 REFERENCE 2 (bases 1 to 5172) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5172 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 8..477 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 542..741 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 942..1358 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1288..1310 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1326..1342 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(1416..1436) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1421..1750 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1601..1736 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1663..1682 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 1782..1791 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1788..2474 /codon_start=1 /product="far-red fluorescent variant of Heteractis crispa chromoprotein" /label=HcRed1 /note="mammalian codon-optimized" /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD LPEKAN" LTR 2666..3135 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(3265..3282) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3436..4024) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3516..3535) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4184..5044) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4807..4826 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5045..5149) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt 901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc 961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg 1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg 1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg 1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag 1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc 1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat 1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt 1381 acgtaggaat tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg 1441 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 1501 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 1561 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 1621 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 1681 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 1741 cttttgcaaa aagcttggta ccgagctcgg atccaccggt cgccaccatg gtgagcggcc 1801 tgctgaagga gagtatgcgc atcaagatgt acatggaggg caccgtgaac ggccactact 1861 tcaagtgcga gggcgagggc gacggcaacc ccttcgccgg cacccagagc atgagaatcc 1921 acgtgaccga gggcgccccc ctgcccttcg ccttcgacat cctggccccc tgctgcgagt 1981 acggcagcag gaccttcgtg caccacaccg ccgagatccc cgacttcttc aagcagagct 2041 tccccgaggg cttcacctgg gagagaacca ccacctacga ggacggcggc atcctgaccg 2101 cccaccagga caccagcctg gagggcaact gcctgatcta caaggtgaag gtgcacggca 2161 ccaacttccc cgccgacggc cccgtgatga agaacaagag cggcggctgg gagcccagca 2221 ccgaggtggt gtaccccgag aacggcgtgc tgtgcggccg gaacgtgatg gccctgaagg 2281 tgggcgaccg gcacctgatc tgccaccact acaccagcta ccggagcaag aaggccgtgc 2341 gcgccctgac catgcccggc ttccacttca ccgacatccg gctccagatg ctgcggaaga 2401 agaaggacga gtacttcgag ctgtacgagg ccagcgtggc ccggtacagc gacctgcccg 2461 agaaggccaa ctgaagcggc cgcgactcta gagggcccgt ttataaaata aaagatttta 2521 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct 2581 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga 2641 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc 2701 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg 2761 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg 2821 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac 2881 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg 2941 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt 3001 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca 3061 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc 3121 agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat 3181 ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 3241 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 3301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 3661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 3781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 3841 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 3901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 4021 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 4081 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc 4141 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 4201 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 4261 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 4321 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 4381 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 4441 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 4501 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 4561 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 4621 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 4681 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 4741 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 4801 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 4861 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 4921 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 4981 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 5041 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 5101 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 5161 gaaaagtgcc ac //