LOCUS Exported 10357 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Flag-hCRM1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10357) AUTHORS Wang W, Budhu A, Forgues M, Wang XW TITLE Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication. JOURNAL Nat Cell Biol. 2005 Aug . 7(8):823-30. PUBMED 16041368 REFERENCE 2 (bases 1 to 10357) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10357 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 51..73 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 65..82 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 66..82 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 243..622 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 623..826 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 777..797 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 856..921 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" polyA_signal 5041..5663 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(5240..5259) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" primer_bind complement(5687..5707) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5705..6002 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind 5934..5953 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 6056..6850 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6110..6129) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6720..6739 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 7502..7636 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7539..7558) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7593..7612 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7641..7660) /label=T7 /note="T7 promoter, forward primer" promoter complement(7642..7660) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7674..7690) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7674..7690) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7687..7709) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7698..7714 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7722..7752) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7767..7788 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7905..7922) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8076..8664) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8156..8175) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8835..9695) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9458..9477 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9696..9800) /gene="bla" /label=AmpR promoter rep_origin 9827..10282 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9914..9933) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10124..10145 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 61 acgttgtaaa acgacggcca gtgccaagct gatctataca ttgaatcaat attggcaatt 121 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac 181 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg 241 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 481 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc 541 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt 601 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata 661 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 721 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca 781 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 841 tcagaattaa ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat 901 tacaaggatg acgatgacaa gcttgcggcc gcgaattcat cgatagatct gatatcggta 961 ccaccagcaa ttatgacaat gttagcagac catgcagctc gtcagctgct tgatttcagc 1021 caaaaactgg atatcaactt attagataat gtggtgaatt gcttatacca tggagaagga 1081 gcccagcaaa gaatggctca agaagtactg acacatttaa aggagcatcc tgatgcttgg 1141 acaagagtcg acacaatttt ggaattttct cagaatatga atacgaaata ctatggacta 1201 caaattttgg aaaatgtgat aaaaacaagg tggaagattc ttccaaggaa ccagtgcgaa 1261 ggaataaaaa aatacgttgt tggcctcatt atcaagacgt catctgaccc aacttgtgta 1321 gagaaagaaa aggtgtatat cggaaaatta aatatgatcc ttgttcagat actgaaacaa 1381 gaatggccca aacattggcc aacttttatc agtgatattg ttggagcaag taggaccagc 1441 gaaagtctct gtcaaaataa tatggtgatt cttaaactct tgagtgaaga agtatttgat 1501 ttctctagtg gacagataac ccaagtcaaa tctaagcatt taaaagacag catgtgcaat 1561 gaattctcac agatatttca actgtgtcag tttgtaatgg aaaattctca aaatgctcca 1621 cttgtacatg caaccttgga aacattgctc agatttctga actggattcc cctgggatat 1681 atttttggga ccaaattaat cagcacattg atttataagt tcctgaatgt tccaatgttt 1741 cgaaatgtct ctctgaagtg cctcactgag attgctggtg tgagtgtaag ccaatatgaa 1801 gaacaatttg taacactatt tactctgaca atgatgcaac taaagcagat gcttccttta 1861 aataccaata ttcgacttgc gtactcaaat gggaaagatg atgaacagaa cttcattcaa 1921 aatctcagtt tgtttctctg cacctttctt aaggaacatg atcaacttat agaaaaaaga 1981 ttaaatctca gggaaactct tatggaggcc cttcattata tgttgttggt atctgaagta 2041 gaagaaactg aaatctttaa aatttgtctt gaatactgga atcatttggc tgctgaactc 2101 tatagagaga gtccattctc tacatctgcc tctccgttgc tttctggaag tcaacatttt 2161 gatgttcctc ccaggagaca gctatatttg cccatgttat tcaaggtccg tttattaatg 2221 gttagtcgaa tggctaaacc agaggaagta ttggttgtag agaatgatca aggagaagtt 2281 gtgagagaat tcatgaagga tacagattcc ataaatttgt ataagaatat gagggaaaca 2341 ttggtttatc ttactcatct ggattatgta gatacagaaa gaataatgac agagaagctt 2401 cacaatcaag tgaatggtac agagtggtca tggaaaaatt tgaatacatt gtgttgggca 2461 ataggctcca ttagtggagc aatgcatgaa gaggacgaaa aacgatttct tgttactgtt 2521 ataaaggatc tattaggatt atgtgaacag aaaagaggca aagataataa agctattatt 2581 gcatcaaata tcatgtacat agtaggtcaa tacccacgtt ttttgagagc tcactggaaa 2641 tttctgaaga ctgtagttaa caagctgttc gaattcatgc atgagaccca tgatggagtc 2701 caggatatgg cttgtgatac tttcattaaa atagcccaaa aatgccgcag gcatttcgtt 2761 caggttcagg ttggagaagt gatgccattt attgatgaaa ttttgaacaa cattaacact 2821 attatttgtg atcttcagcc tcaacaggtt catacgtttt atgaagctgt ggggtacatg 2881 attggtgcac aaacagatca aacagtacaa gaacacttga tagaaaagta catgttactc 2941 cctaatcaag tgtgggatag tataatccag caggcaacca aaaatgtgga tatactgaaa 3001 gatcctgaaa cagtcaagca gcttggtagc attttgaaaa caaatgtgag agcctgcaaa 3061 gctgttggac acccctttgt aattcagctt ggaagaattt atttagatat gcttaatgta 3121 tacaagtgcc tcagtgaaaa tatttctgca gctatccaag ctaatggtga aatggttaca 3181 aagcaaccat tgattagaag tatgcgaact gtaaaaaggg aaactttaaa gttaatatct 3241 ggttgggtga gccgatccaa tgatccacag atggtcgctg aaaattttgt tccccctctg 3301 ttggatgcag ttctcattga ttatcagaga aatgtcccag ctgctagaga accagaagtg 3361 cttagtacta tggccataat tgtcaacaag ttagggggac atataacagc tgaaatacct 3421 caaatatttg atgctgtttt tgaatgcaca ttgaatatga taaataagga ctttgaagaa 3481 tatcctgaac atagaacgaa ctttttctta ctacttcagg ctgtcaattc tcattgtttc 3541 ccagcattcc ttgctattcc acctacacag tttaaacttg ttttggattc catcatttgg 3601 gctttcaaac atactatgag gaatgtcgca gatacgggct tacagatact ttttacactc 3661 ttacaaaatg ttgcacaaga agaagctgca gctcagagtt tttatcaaac ttatttttgt 3721 gatattctcc agcatatctt ttctgttgtg acagacactt cacatactgc tggtttaaca 3781 atgcatgcat caattcttgc atatatgttt aatttggttg aagaaggaaa aataagtaca 3841 tcattaaatc ctggaaatcc agttaacaac caaatctttc ttcaggaata tgtggctaat 3901 ctccttaagt cggccttccc tcacctacaa gatgctcaag taaagctctt tgtgacaggg 3961 cttttcagct taaatcaaga tattcctgct ttcaaggaac atttaagaga tttcctagtt 4021 caaataaagg aatttgcagg tgaagacact tctgatttgt ttttggaaga gagagaaata 4081 gccctacggc aggctgatga agagaaacat aaacgtcaaa tgtctgtccc tggcatcttt 4141 aatccacatg agattccaga agaaatgtgt gattaaaatc caaattcatg ctgttttttt 4201 tctctgcaac tcgttagcag aggaaaacag catgtgggta tttgtcggcc aaaatgatgc 4261 caatttgtaa attaaaatgt cacctagtgg ccctttttct tatgtgtttt tttgtataag 4321 aaattttctg tgaaatatcc ttccattgtt taagcttttg ttttggtcat ctttatttag 4381 tttgcatgaa gttgaaaatt aaggcatttt taaaaatttt acttcatgcc catttttgtg 4441 gctgggctgg ggggaggagg caaattcgat ttgaacatat acttgtaatt ctaatgcaaa 4501 attatacaat ttttcctgta aacaatacca atttttaatt agggagcatt ttccttctag 4561 tctatttcag cctagaagaa aagataatga gtaaaacaaa ttgcgttgtt taaaggatta 4621 tagtgctgca ttgtctgaag ttagcacctc ttggactgaa tcgtttgtct agactacatg 4681 tattacaaag tctctttggc aagattgcag caagatcatg tgcatatcat cccattgtaa 4741 agcgacttca aaaatatggg aacacagtta gttattttta cacagttctt tttgtttttg 4801 tgtgtgtgtg ctgtcgcttg tcgacaacag ctttttgttt tcctcaatga ggagtgttgc 4861 tcatttgtga gccttcatta actcgaagtg aaatggttaa aaatatttat cctgttagaa 4921 taggctgcat ctttttaaca actcattaaa aaacaaaaca actctggctt ttgagatgac 4981 ttatactaat ttacattgtt taccaagctg tagtgcttta agaacactac gggggatccc 5041 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca 5101 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc 5161 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca 5221 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg 5281 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg 5341 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg 5401 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct 5461 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg 5521 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat 5581 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc 5641 actcagtaga tgcctgttga attgggtacg cggccagctt ggctgtggaa tgtgtgtcag 5701 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 5761 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 5821 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 5881 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 5941 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 6001 ggaggaattg atcagcttgg gatctgatca agagacagga tgaggatcgt ttcgcatgat 6061 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 6121 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 6181 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 6241 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 6301 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 6361 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 6421 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 6481 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 6541 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 6601 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 6661 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 6721 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 6781 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 6841 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 6901 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 6961 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 7021 cccgggctcg atcccctcgc gagttggttc agctgctgcc tgaggctgga cgacctcgcg 7081 gagttctacc ggcagtgcaa atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc 7141 atccatgccc ccgaactgca ggagtgggga ggcacgatgg ccgctttggt cgacccggac 7201 gggacgctcc tgcgcctgat acagaacgaa ttgcttgcag gcatctcatg agtgtgtctt 7261 cccgttttcc gcctgaggtc actgcgtgga tggagcgctg gcgcctgctg cgcgacggcg 7321 agctgctcac cacccactcg ccaagctgga accgtaaaaa ggccgcgttg ctggcgtttt 7381 tccataggct ccgccgatca taatcagcca taccacattt gtagaggttt tacttgcttt 7441 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 7501 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 7561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 7621 ttatcatgtc tggatcaatt ccctatagtg agtcgtatta aattcgtaat catgtcatag 7681 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 7741 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 7801 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 7861 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 7921 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 7981 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 8041 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 8101 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 8161 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 8221 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 8281 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 8341 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 8401 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 8461 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 8521 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 8581 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 8641 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 8701 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 8761 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 8821 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 8881 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 8941 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 9001 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 9061 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 9121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 9181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 9241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 9301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 9361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 9421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 9481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 9541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 9601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 9661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 9721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 9781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc 9841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 9901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 9961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 10021 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 10081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 10141 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 10201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 10261 aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc 10321 gatcggtgcg ggcctcttcg ctattacgcc agctggc //