LOCUS Exported 10438 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087). ACCESSION . VERSION . KEYWORDS pEPOR2KN0061 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10438) AUTHORS Raitskin O, Schudoma C, West A, Patron NJ TITLE Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. JOURNAL PLoS One. 2019 Feb 27;14(2):e0211598. doi: 10.1371/journal.pone.0211598. eCollection 2019. PUBMED 30811422 REFERENCE 2 (bases 1 to 10438) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10438 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 454..799 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 742..762 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 896..916 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 941..4096 /codon_start=1 /product="Cas9 endonuclease from the Staphylococcus aureus Type II CRISPR/Cas system" /label=SaCas9 /note="generates RNA-guided double strand breaks in DNA" /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI IKKG" CDS 4097..4144 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 4151..4231 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA" CDS 4238..4957 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4283..4304) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4544..4563) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4891..4912 /label=EGFP-C /note="EGFP, forward primer" terminator 4970..5222 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_RNA 5476..5551 /label=Sa gRNA scaffold /note="guide RNA scaffold for the Staphylococcus aureus CRISPR/Cas9 system" misc_feature 5630..5654 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 5963..6592 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 7021..8094 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 8160..8354 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" promoter 8562..8666 /gene="bla" /label=AmpR promoter CDS 8667..9461 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin 9632..10220 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10121..10140 /label=pBR322ori-F /note="pBR322 origin, forward primer" misc_feature 10356..10380 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" ORIGIN 1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt 61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag 121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc 181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca 241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga 301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa 361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt 421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc 481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga 541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga 601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt 721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca 841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggccccaaa 901 gaagaagcgg aaggtcggta tccacggagt cccagcagcc aagcggaact acatcctggg 961 cctggacatc ggcatcacca gcgtgggcta cggcatcatc gactacgaga cacgggacgt 1021 gatcgatgcc ggcgtgcggc tgttcaaaga ggccaacgtg gaaaacaacg agggcaggcg 1081 gagcaagaga ggcgccagaa ggctgaagcg gcggaggcgg catagaatcc agagagtgaa 1141 gaagctgctg ttcgactaca acctgctgac cgaccacagc gagctgagcg gcatcaaccc 1201 ctacgaggcc agagtgaagg gcctgagcca gaagctgagc gaggaagagt tctctgccgc 1261 cctgctgcac ctggccaaga gaagaggcgt gcacaacgtg aacgaggtgg aagaggacac 1321 cggcaacgag ctgtccacca aagagcagat cagccggaac agcaaggccc tggaagagaa 1381 atacgtggcc gaactgcagc tggaacggct gaagaaagac ggcgaagtgc ggggcagcat 1441 caacagattc aagaccagcg actacgtgaa agaagccaaa cagctgctga aggtgcagaa 1501 ggcctaccac cagctggacc agagcttcat cgacacctac atcgacctgc tggaaacccg 1561 gcggacctac tatgagggac ctggcgaggg cagccccttc ggctggaagg acatcaaaga 1621 atggtacgag atgctgatgg gccactgcac ctacttcccc gaggaactgc ggagcgtgaa 1681 gtacgcctac aacgccgacc tgtacaacgc cctgaacgac ctgaacaatc tcgtgatcac 1741 cagggacgag aacgagaagc tggaatatta cgagaagttc cagatcatcg agaacgtgtt 1801 caagcagaag aagaagccca ccctgaagca gatcgccaaa gaaatcctcg tgaacgaaga 1861 ggatattaag ggctacagag tgaccagcac cggcaagccc gagttcacca acctgaaggt 1921 gtaccacgac atcaaggaca ttaccgcccg gaaagagatt attgagaacg ccgagctgct 1981 ggatcagatt gccaagatcc tgaccatcta ccagagcagc gaggacatcc aggaagaact 2041 gaccaatctg aactccgagc tgacccagga agagatcgag cagatctcta atctgaaggg 2101 ctataccggc acccacaacc tgagcctgaa ggccatcaac ctgatcctgg acgagctgtg 2161 gcacaccaac gacaaccaga tcgctatctt caaccggctg aagctggtgc ccaagaaggt 2221 ggacctgtcc cagcagaaag agatccccac caccctggtg gacgacttca tcctgagccc 2281 cgtcgtgaag agaagcttca tccagagcat caaagtgatc aacgccatca tcaagaagta 2341 cggcctgccc aacgacatca ttatcgagct ggcccgcgag aagaactcca aggacgccca 2401 gaaaatgatc aacgagatgc agaagcggaa ccggcagacc aacgagcgga tcgaggaaat 2461 catccggacc accggcaaag agaacgccaa gtacctgatc gagaagatca agctgcacga 2521 catgcaggaa ggcaagtgcc tgtacagcct ggaagccatc cctctggaag atctgctgaa 2581 caaccccttc aactatgagg tggaccacat catccccaga agcgtgtcct tcgacaacag 2641 cttcaacaac aaggtgctcg tgaagcagga agaaaacagc aagaagggca accggacccc 2701 attccagtac ctgagcagca gcgacagcaa gatcagctac gaaaccttca agaagcacat 2761 cctgaatctg gccaagggca agggcagaat cagcaagacc aagaaagagt atctgctgga 2821 agaacgggac atcaacaggt tctccgtgca gaaagacttc atcaaccgga acctggtgga 2881 taccagatac gccaccagag gcctgatgaa cctgctgcgg agctacttca gagtgaacaa 2941 cctggacgtg aaagtgaagt ccatcaatgg cggcttcacc agctttctgc ggcggaagtg 3001 gaagtttaag aaagagcgga acaaggggta caagcaccac gccgaggacg ccctgatcat 3061 tgccaacgcc gatttcatct tcaaagagtg gaagaaactg gacaaggcca aaaaagtgat 3121 ggaaaaccag atgttcgagg aaaagcaggc cgagagcatg cccgagatcg aaaccgagca 3181 ggagtacaaa gagatcttca tcacccccca ccagatcaag cacattaagg acttcaagga 3241 ctacaagtac agccaccggg tggacaagaa gcctaataga gagctgatta acgacaccct 3301 gtactccacc cggaaggacg acaagggcaa caccctgatc gtgaacaatc tgaacggcct 3361 gtacgacaag gacaatgaca agctgaaaaa gctgatcaac aagagccccg aaaagctgct 3421 gatgtaccac cacgaccccc agacctacca gaaactgaag ctgattatgg aacagtacgg 3481 cgacgagaag aatcccctgt acaagtacta cgaggaaacc gggaactacc tgaccaagta 3541 ctccaaaaag gacaacggcc ccgtgatcaa gaagattaag tattacggca acaaactgaa 3601 cgcccatctg gacatcaccg acgactaccc caacagcaga aacaaggtcg tgaagctgtc 3661 cctgaagccc tacagattcg acgtgtacct ggacaatggc gtgtacaagt tcgtgaccgt 3721 gaagaatctg gatgtgatca aaaaagaaaa ctactacgaa gtgaatagca agtgctatga 3781 ggaagctaag aagctgaaga agatcagcaa ccaggccgag tttatcgcct ccttctacaa 3841 caacgatctg atcaagatca acggcgagct gtatagagtg atcggcgtga acaacgacct 3901 gctgaaccgg atcgaagtga acatgatcga catcacctac cgcgagtacc tggaaaacat 3961 gaacgacaag aggcccccca ggatcattaa gacaatcgcc tccaagaccc agagcattaa 4021 gaagtacagc acagacattc tgggcaacct gtatgaagtg aaatctaaga agcaccctca 4081 gatcatcaaa aagggcaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa 4141 aaagggatcc tacccatacg atgttccaga ttacgcttac ccatacgatg ttccagatta 4201 cgcttaccca tacgatgttc cagattacgc tggttcgatg gtgagcaagg gcgaggagct 4261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 4321 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 4381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg 4441 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc 4501 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 4561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 4621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 4681 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 4741 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 4801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct 4861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 4921 cgggatcact ctcggcatgg acgagctgta caagtgagct tgtcaagcag atcgttcaaa 4981 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat 5041 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt 5101 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa 5161 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga 5221 tcgacgctac tagaattcga gctcggagca tcttcattct taagatatga agataatctt 5281 caaaaggccc ctgggaatct gaaagaagag aagcaggccc atttatatgg gaaagaacaa 5341 tagtatttct tatataggcc catttaagtt gaaaacaatc ttcaaaagtc ccacatcgct 5401 tagataagaa aacgaagctg agtttatata cagctagagt cgaagtagtg attgtatgac 5461 ctttaagaga gaagcgtttt agtactctgg aaacagaatc tactaaaaca aggcaaaatg 5521 ccgtgtttat ctcgtcaact tgttggcgag atttttttac ttgtctcgct ttacgaggat 5581 gcacatgtga ccgagggaca cgaagtgatc cgtttaaact atcagtgttt gacaggatat 5641 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg 5701 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc 5761 gggagtcagc cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg 5821 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg 5881 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata 5941 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc 6001 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt 6061 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga 6121 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat 6181 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc 6241 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg 6301 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct 6361 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc 6421 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag 6481 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc 6541 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga 6601 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc 6661 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac 6721 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag 6781 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga 6841 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc 6901 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag 6961 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc 7021 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg 7081 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc 7141 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg 7201 gcagccggtg cgtcgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc 7261 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt 7321 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac 7381 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg 7441 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga 7501 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga 7561 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg 7621 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag 7681 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac 7741 atcgagatcg agctagctga ttggatgtac cgcgagatta cagaaggcaa gaacccggac 7801 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac 7861 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac 7921 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc 7981 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc 8041 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg 8101 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaaag cttctttcct 8161 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt 8221 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag 8281 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc 8341 ctggcctgtg cataactgtc tggccagcgc acagccgaac agctgcaaaa agcgcccaca 8401 tcaaggctcc gagtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 8461 tatccgctca tgagacaata acccksctgg cctgtgcata actgtctggc cagcgcacag 8521 ccgaacagct gcaaaaagcg cccacatcaa ggctccgagt gcgcggaacc cctatttgtt 8581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 8641 ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag aatatcaccg gaattgaaaa 8701 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat 8761 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag 8821 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc 8881 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg 8941 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta 9001 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct 9061 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg 9121 ccgatgtgga ttgcgaaaac tgggaagagg acactccatt taaagatccg cgcgagctgt 9181 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg 9241 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa 9301 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgctc agggaggata 9361 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg 9421 agaaaataaa atattatatt ttactggatg aattgtttta gctgtcagac caagtttact 9481 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 9541 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 9601 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 9661 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 9721 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 9781 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 9841 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 9901 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 9961 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 10021 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 10081 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 10141 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 10201 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctgctcggat 10261 ctgttggacc ggacagtagt catggttgat gggctgcctg tatcgagtgg tgattttgtg 10321 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac 10381 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggtt //