LOCUS Exported 11625 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104). ACCESSION . VERSION . KEYWORDS pEPOR2KN0071 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11625) AUTHORS Raitskin O, Schudoma C, West A, Patron NJ TITLE Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. JOURNAL PLoS One. 2019 Feb 27;14(2):e0211598. doi: 10.1371/journal.pone.0211598. eCollection 2019. PUBMED 30811422 REFERENCE 2 (bases 1 to 11625) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11625 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 454..799 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 742..762 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 890..4993 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5006..5026 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5033..5752 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5078..5099) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5339..5358) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5686..5707 /label=EGFP-C /note="EGFP, forward primer" terminator 5765..6017 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_RNA 6270..6345 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" protein_bind complement(6357..6381) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_RNA 6637..6712 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" protein_bind complement(6724..6748) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 6817..6841 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 7150..7779 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 8208..9281 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 9347..9541 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" promoter 9749..9853 /gene="bla" /label=AmpR promoter CDS 9854..10648 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin 10819..11407 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11308..11327 /label=pBR322ori-F /note="pBR322 origin, forward primer" misc_feature 11543..11567 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" ORIGIN 1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt 61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag 121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc 181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca 241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga 301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa 361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt 421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc 481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga 541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga 601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt 721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca 841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggacaagaa 901 gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga 961 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa 1021 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct 1081 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga 1141 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc 1201 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga 1261 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga 1321 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt 1381 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact 1441 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc 1501 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa 1561 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct 1621 gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct 1681 tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga 1741 ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga 1801 tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg 1861 ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc 1921 tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga 1981 cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga 2041 cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac 2101 tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag 2161 gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct 2221 cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg 2281 gatgactcgc aaatcagaag agactatcac tccctggaac ttcgaggaag tcgtggataa 2341 gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa 2401 cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct 2461 caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca 2521 gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct 2581 caaagaagat tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga 2641 ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa 2701 ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac 2761 gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga 2821 cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag 2881 aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa 2941 gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt 3001 taaggaggac atccagaaag cacaagtttc tggccagggg gacagtctcc acgagcacat 3061 cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt 3121 ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc 3181 ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat 3241 tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac 3301 ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt 3361 ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca 3421 gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag 3481 agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg 3541 gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga 3601 acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac 3661 acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga 3721 tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtttc 3781 agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc 3841 gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct 3901 tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa 3961 gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa 4021 ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga 4081 aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg 4141 gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg 4201 cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa 4261 agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact 4321 ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct 4381 gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc 4441 gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt 4501 tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa 4561 cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa 4621 gctcaaagga tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca 4681 ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga 4741 cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga 4801 gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt 4861 caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga 4921 cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca 4981 gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgggtt cgatggtgag 5041 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 5101 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 5161 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 5221 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga 5281 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 5341 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 5401 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 5461 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 5521 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 5581 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 5641 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 5701 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt gagcttgtca 5761 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct 5821 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta 5881 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta 5941 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc 6001 atctatgtta ctagatcgac gctactagaa ttcgagctcg gagcatcttc attcttaaga 6061 tatgaagata atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta 6121 tatgggaaag aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa 6181 aagtcccaca tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag 6241 tagtgattgc gacagtgata gttagggccg ttttagagct agaaatagca agttaaaata 6301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttctagaccc 6361 agctttcttg tacaaagttg gcattacgct ttacgaattc ccatggggag catcttcatt 6421 cttaagatat gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag agaagcaggc 6481 ccatttatat gggaaagaac aatagtattt cttatatagg cccatttaag ttgaaaacaa 6541 tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata tacagctaga 6601 gtcgaagtag tgattgtcat gtgcgatgag aaaatcgttt tagagctaga aatagcaagt 6661 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc 6721 tagacccagc tttcttgtac aaagttggca ttacgctcag agaggatgca catgtgaccg 6781 agggacacga agtgatccgt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa 6841 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt 6901 atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg agtcagccgt 6961 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag 7021 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa 7081 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa 7141 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc 7201 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat 7261 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc 7321 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac 7381 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca 7441 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac 7501 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt 7561 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc 7621 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact 7681 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc 7741 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa 7801 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg 7861 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg 7921 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc 7981 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga 8041 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc 8101 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc 8161 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca 8221 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga 8281 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt 8341 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgt 8401 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct 8461 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga 8521 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg 8581 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg 8641 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc 8701 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa 8761 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc 8821 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga 8881 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc 8941 tagctgattg gatgtaccgc gagattacag aaggcaagaa cccggacgtg ctgacggttc 9001 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc 9061 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca 9121 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc 9181 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct 9241 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag 9301 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaagctt ctttcctgtg gatagcacgt 9361 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc 9421 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt 9481 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat 9541 aactgtctgg ccagcgcaca gccgaacagc tgcaaaaagc gcccacatca aggctccgag 9601 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 9661 gacaataacc ctgatggcct gtgcataact gtctggccag cgcacagccg aacagctgca 9721 aaaagcgccc acatcaaggc tccgagtgcg cggaacccct atttgtttat ttttctaaat 9781 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 9841 aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa 9901 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg 9961 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga 10021 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt 10081 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt 10141 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc 10201 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt 10261 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg 10321 cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg attttttaaa 10381 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat 10441 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga 10501 caagtggtat gacattgcct tctgcgtccg gtcgctcagg gaggatatcg gggaagaaca 10561 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata 10621 ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat atatacttta 10681 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 10741 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 10801 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 10861 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 10921 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 10981 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 11041 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 11101 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 11161 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 11221 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 11281 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 11341 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 11401 atggaaaaac gccagcaacg cggccttttt acggttcctg ctcggatctg ttggaccgga 11461 cagtagtcat ggttgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt 11521 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta 11581 gacaacttaa taacacattg cggacgtttt taatgtactg gggtt //