LOCUS Exported 3043 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Plasmid containing shortened version of V-D CRISPR array for integration assays. ACCESSION . VERSION . KEYWORDS pVD_CRISPR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3043) AUTHORS Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA TITLE A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System. JOURNAL Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29. PUBMED 30709710 REFERENCE 2 (bases 1 to 3043) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3043 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 15..31 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 15..31 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" misc_feature 44..100 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(101..118) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(101..117) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(110..132) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(326..345) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 445..467 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(505..523) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 591..695 /gene="bla" /label=AmpR promoter CDS 696..1556 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(914..933) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1727..2315 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2216..2235 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2469..2486 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca 61 ggtcgactct agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac 121 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 181 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 241 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 301 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 361 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 421 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 481 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 1081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 1141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 1201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 1261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 1321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 1381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 1441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 1501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 1561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 1621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 1681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 1741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 1801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 1861 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 1921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 1981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 2221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 2281 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 2341 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 2401 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 2461 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcggg 2521 ttataccttc ttgtaactag gggcaggggt aaagatacgc ggaaacagag tggaacaggc 2581 ggagacactc ccagtcgtgc tagacttgaa aaacagaata tgccggagtg atgagtggga 2641 aggctagttc tctttctcgc tctcaacgga gctcaggaga atggaaccat catcattttt 2701 ttgcggggat acaaaaggaa agagtttcgg gcttgaattg acaagcatgc ctttttccct 2761 ctaatcttcg gggatcaata ccaaactctg ggcctttttc cctctaatct tcggggagat 2821 aaggctagag aaaaacctgc tttaccccat aatcagaggt tccccggcat attctatttt 2881 caaagaacaa tggaatagag cggaaaaaac cctccgtttc tatcccgttt ttgatacgtt 2941 tcccaagaaa tgcatgtcac tgcgaaccta catcattttc taaaataaga caagcattat 3001 ttttactaag caattaagca taccagacgc tctccatgat tat //