LOCUS       Exported                1080 bp ds-DNA     linear   SYN 10-JUN-2021
DEFINITION  Barcoded lentiviral vector to express POU5F1 in mammalian cells 
            under control of EF1a promoter. Barcoded for transcript detection in
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    EF1a_POU5F1_P2A_Hygro_Barcode
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 atggcgggac acctggcttc agattttgcc ttctcgcccc ctccaggtgg tggaggtgat
       61 gggccagggg ggccggagcc gggctgggtt gatcctcgga cctggctaag cttccaaggc
      121 cctcctggag ggccaggaat cgggccgggg gttgggccag gctctgaggt gtgggggatt
      181 cccccatgcc ccccgccgta tgagttctgt ggggggatgg cgtactgtgg gccccaggtt
      241 ggagtggggc tagtgcccca aggcggcttg gagacctctc agcctgaggg cgaagcagga
      301 gtcggggtgg agagcaactc cgatggggcc tccccggagc cctgcaccgt cacccctggt
      361 gccgtgaagc tggagaagga gaagctggag caaaacccgg aggagtccca ggacatcaaa
      421 gctctgcaga aagaactcga gcaatttgcc aagctcctga agcagaagag gatcaccctg
      481 ggatatacac aggccgatgt ggggctcacc ctgggggttc tatttgggaa ggtattcagc
      541 caaacgacca tctgccgctt tgaggctctg cagcttagct tcaagaacat gtgtaagctg
      601 cggcccttgc tgcagaagtg ggtggaggaa gctgacaaca atgaaaatct tcaggagata
      661 tgcaaagcag aaaccctcgt gcaggcccga aagagaaagc gaaccagtat cgagaaccga
      721 gtgagaggca acctggagaa tttgttcctg cagtgcccga aacccacact gcagcagatc
      781 agccacatcg cccagcagct tgggctcgag aaggatgtgg tccgagtgtg gttctgtaac
      841 cggcgccaga agggcaagcg atcaagcagc gactatgcac aacgagagga ttttgaggct
      901 gctgggtctc ctttctcagg gggaccagtg tcctttcctc tggccccagg gccccatttt
      961 ggtaccccag gctatgggag ccctcacttc actgcactgt actcctcggt ccctttccct
     1021 gagggggaag cctttccccc tgtctctgtc accactctgg gctctcccat gcattcaaac
//
