LOCUS Exported 5790 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581. ACCESSION . VERSION . KEYWORDS tdEos-CENPA-C-22 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5790) TITLE Davidson Photoactivatable Fluorescent Proteins REFERENCE 2 (bases 1 to 5790) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5790 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 553..1230 /codon_start=1 /product="green-to-red photoswitchable fluorescent protein (Wiedenmann et al., 2004)" /label=EosFP /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" polyA_signal 2518..2639 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2555..2574) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2609..2628 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2646..3101) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2783..2804) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2995..3014 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3128..3232 /gene="bla" /label=AmpR promoter promoter 3234..3591 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3257..3277) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3442..3577 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3504..3523 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3626..4420 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3680..3699) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4290..4309 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4608..4627) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4652..4699 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5028..5616 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5517..5536 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc 601 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga 661 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc 721 ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa 781 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa 841 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat 901 aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg 961 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat 1021 attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact 1081 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt 1141 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct 1201 cattctggat tgcctgacaa tgccagacga tccggtagcg gtcacggtac tggttctact 1261 ggttctggtt cttctgagag tgcgattaag ccagacatga agatcaacct ccgtatggaa 1321 ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag 1381 ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat 1441 atcctgacca ctgcattcca ttacggcaac agggtattcg ccgaatatcc agaccacata 1501 caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc 1561 gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat 1621 aataaagttc gatttcacgg tgtaaacttt cccgccaatg gtccagttat gcagaagaag 1681 acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgactggt 1741 gatattcgca tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact 1801 acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc 1861 attgagattt taagccatga caaagattac aacaaggtta agctgtatga ggcttcgaat 1921 tctagcagtt ctagagtaag cagcagagta agtggcccgc gccgccggag ccgaaagccc 1981 gaggccccga ggaggcgcag cccgagcccg accccgaccc ccggcccctc ccggcggggc 2041 ccctccttag gcgcttcctc ccatcaacac agtcggcgga gacaaggttg gctaaaggag 2101 atccgaaagc ttcagaagag cacacacctc ttgataagga agctgccctt cagccgcctg 2161 gcaagagaaa tatgtgttaa attcactcgt ggtgtggact tcaattggca agcccaggcc 2221 ctattggccc tacaagaggc agcagaagca tttctagttc atctctttga ggacgcctat 2281 ctcctcacct tacatgcagg ccgagttact ctcttcccaa aggatgtgca actggcccgg 2341 aggatccggg gccttgagga gggactcggc taaggtaccg cgggcccggg atccaccgga 2401 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 2461 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 2521 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 2581 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 2641 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 2701 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 2761 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 2821 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 2881 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 2941 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 3001 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 3061 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 3121 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3181 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 3241 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 3301 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 3361 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 3421 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 3481 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 3541 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca 3601 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 3661 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 3721 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 3781 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 3841 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 3901 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 3961 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 4021 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 4081 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 4141 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 4201 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 4261 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 4321 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 4381 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 4441 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 4501 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 4561 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag 4621 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac 4681 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat 4741 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac 4801 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc 4861 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 4921 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 4981 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 5041 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 5101 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 5161 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 5221 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 5281 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 5341 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 5401 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 5461 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 5521 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 5581 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 5641 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 5701 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc 5761 attagttcat agcccatata tggagttccg //