LOCUS Exported 11225 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668). ACCESSION . VERSION . KEYWORDS pZ M2rtTA_CAGG TetON-Sox10 with GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11225) AUTHORS Garcia-Leon JA, Kumar M, Boon R, Chau D, One J, Wolfs E, Eggermont K, Berckmans P, Gunhanlar N, de Vrij F, Lendemeijer B, Pavie B, Corthout N, Kushner SA, Davila JC, Lambrichts I, Hu WS, Verfaillie CM TITLE SOX10 Single Transcription Factor-Based Fast and Efficient Generation of Oligodendrocytes from Human Pluripotent Stem Cells. JOURNAL Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11. PUBMED 29337119 REFERENCE 2 (bases 1 to 11225) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11225 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..19 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 36..54 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 72..90 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 108..126 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 143..161 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 179..197 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" promoter 209..247 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 244..268 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 276..285 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1707..1763 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 1776..2495 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 1776..2492 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1821..1842) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2082..2101) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2429..2450 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(2515..2534) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 2559..2606 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" polyA_signal 2861..2972 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" CDS complement(3126..3725) /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3209..3229) /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind 3706..3725 /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" CDS complement(3735..3788) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" misc_feature 3809..3834 /label=SA /note="splice acceptor site" misc_feature 3878..4681 /label=HA-L /note="left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" primer_bind complement(4695..4714) /label=T7 /note="T7 promoter, forward primer" promoter complement(4696..4714) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4724..4741) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4724..4740) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4733..4755) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4882..5337 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4969..4988) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5179..5200 /label=F1ori-F /note="F1 origin, forward primer" promoter 5363..5467 /gene="bla" /label=AmpR promoter CDS 5468..6328 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5686..5705) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6499..7087 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6988..7007 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7241..7258 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7375..7396 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7411..7441 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7449..7465 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7454..7476 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7473..7489 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7473..7489 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7508..7528 /label=T3 /note="T3 promoter, forward primer" promoter 7510..7528 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" misc_feature 7558..8394 /label=HA-R /note="right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" protein_bind complement(8404..8437) /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" polyA_signal 8444..8668 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" CDS complement(8682..9428) /codon_start=1 /product="improved tetracycline-controlled transactivator" /label=rtTA-Advanced /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" primer_bind complement(9456..9475) /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" intron 9483..10499 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind complement(9537..9559) /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" promoter complement(10500..10777) /label=chicken beta-actin promoter enhancer 10782..11085 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat 61 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga 121 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc 181 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag 241 cagagctcgt ttagtgaacc gtcagatcgc ttaaggccac catggcggag gagcaggacc 301 tatcggaggt ggagctgagc cccgtgggct cggaggagcc ccgctgcctg tccccgggga 361 gcgcgccctc gctagggccc gacggcggcg gcggcggatc gggcctgcga gccagcccgg 421 ggccaggcga gctgggcaag gtcaagaagg agcagcagga cggcgaggcg gacgatgaca 481 agttccccgt gtgcatccgc gaggccgtca gccaggtgct cagcggctac gactggacgc 541 tggtgcccat gcccgtgcgc gtcaacggcg ccagcaaaag caagccgcac gtcaagcggc 601 ccatgaacgc cttcatggtg tgggctcagg cagcgcgcag gaagctcgcg gaccagtacc 661 cgcacctgca caacgctgag ctcagcaaga cgctgggcaa gctctggagg ctgctgaacg 721 aaagtgacaa gcgccccttc atcgaggagg ctgagcggct ccgtatgcag cacaagaaag 781 accacccgga ctacaagtac cagcccaggc ggcggaagaa cgggaaggcc gcccagggcg 841 aggcggagtg ccccggtggg gaggccgagc aaggtgggac cgccgccatc caggcccact 901 acaagagcgc ccacttggac caccggcacc caggagaggg ctcccccatg tcagatggga 961 accccgagca cccctcaggc cagagccatg gcccacccac ccctccaacc accccgaaga 1021 cagagctgca gtcgggcaag gcagacccga agcgggacgg gcgctccatg ggggagggcg 1081 ggaagcctca catcgacttc ggcaacgtgg acattggtga gatcagccac gaggtaatgt 1141 ccaacatgga gacctttgat gtggctgagt tggaccagta cctgccgccc aatgggcacc 1201 caggccacgt gagcagctac tcagcagccg gctatgggct gggcagtgcc ctggccgtgg 1261 ccagtggaca ctccgcctgg atctccaagc caccaggcgt ggctctgccc acggtctcac 1321 cacctggtgt ggatgccaaa gcccaggtga agacagagac cgcggggccc caggggcccc 1381 cacactacac cgaccagcca tccacctcac agatcgccta cacctccctc agcctgcccc 1441 actatggctc agccttcccc tccatctccc gcccccagtt tgactactct gaccatcagc 1501 cctcaggacc ctattatggc cactcgggcc aggcctctgg cctctactcg gccttctcct 1561 atatggggcc ctcgcagcgg cccctctaca cggccatctc tgaccccagc ccctcagggc 1621 cccagtccca cagccccaca cactgggagc agccagtata tacgacactg tcccggcccg 1681 atatcggtgg tggctcgggg ggaggagcga caaactttag cttgctgaag caagctggtg 1741 acgttgagga gaatcccgga ccaggattca ccggtatggt gagcaagggc gaggagctgt 1801 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 1861 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 1921 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 1981 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 2041 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 2101 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 2161 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 2221 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 2281 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 2341 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 2401 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 2461 ggatcactct cggcatggac gagctgtaca agtaaacgcg tgggggaggc taactgaaac 2521 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 2581 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 2641 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 2701 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 2761 ccctgccata gctcgagtgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac 2821 cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta 2881 ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca 2941 aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggg tttaaacggg 3001 ccctctagag tcggtgggcc tcgggggcgg gtgcggggtc ggcggggccg ccccgggtgg 3061 cttcggtcgg agccatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc 3121 gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg 3181 acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag 3241 gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg 3301 gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac 3361 gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc 3421 cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg 3481 acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg 3541 ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg 3601 tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg 3661 gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg 3721 gtcatttcga gcctagggcc gggattctcc tccacgtcac cgcatgttag aagacttcct 3781 ctgccctctc cgctgccaga tctgaggccc tgtgggagga agagaagagg tcaggccgaa 3841 gttcctatac tatttgaaga ataggaactt caagcttgcc ccactgtggg gtggagggga 3901 cagataaaag tacccagaac cagagccaca ttaaccggcc ctgggaatat aaggtggtcc 3961 cagctcgggg acacaggatc cctggaggca gcaaacatgc tgtcctgaag tggacatagg 4021 ggcccgggtt ggaggaagaa gactagctga gctctcggac ccctggaaga tgccatgaca 4081 gggggctgga agagctagca cagactagag aggtaagggg ggtaggggag ctgcccaaat 4141 gaaaggagtg agaggtgacc cgaatccaca ggagaacggg gtgtccaggc aaagaaagca 4201 agaggatgga gaggtggcta aagccaggga gacggggtac tttggggttg tccagaaaaa 4261 cggtgatgat gcaggcctac aagaagggga ggcgggacgc aagggagaca tccgtcggag 4321 aaggccatcc taagaaacga gagatggcac aggccccaga aggagaagga aaagggaacc 4381 cagcgagtga agacggcatg gggttgggtg agggaggaga gatgcccgga gaggacccag 4441 acacggggag gatccgctca gaggacatca cgtggtgcag cgccgagaag gaagtgctcc 4501 ggaaagagca tccttgggca gcaacacagc agagagcaag gggaagaggg agtggaggaa 4561 gacggaacct gaaggaggcg gcagggaagg atctgggcca gccgtagagg tgacccaggc 4621 cacaagctgc agacagaaag cggcacaggc ccaggggaga gaatgctggt cagagaaagc 4681 aggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac 4741 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 4801 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 4861 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 4921 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 4981 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 5041 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 5101 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 5161 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 5221 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 5281 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg 5341 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 5401 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 5461 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 5521 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 5581 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 5641 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 5701 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 5761 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 5821 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 5881 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 5941 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 6001 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 6061 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 6121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 6181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 6241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 6301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 6361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 6421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 6481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 6541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 6601 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 6661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 6721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 6781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 6841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 6901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 6961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 7021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 7081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 7141 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 7201 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 7261 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 7321 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 7381 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 7441 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 7501 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggaga 7561 gcagagccag gaacccctgt agggaagggg caggagagcc aggggcatga gatggtggac 7621 gaggaagggg gacagggaag cctgagcgcc tctcctgggc ttgccaagga ctcaaaccca 7681 gaagcccaga gcagggcctt agggaagcgg gaccctgctc tgggcggagg aatatgtccc 7741 agatagcact ggggactctt taaggaaaga aggatggaga aagagaaagg gagtagaggc 7801 ggccacgacc tggtgaacac ctaggacgca ccattctcac aaagggagtt ttccacacgg 7861 acacccccct cctcaccaca gccctgccag gacggggctg gctactggcc ttatctcaca 7921 ggtaaaactg acgcacggag gaacaatata aattggggac tagaaaggtg aagagccaaa 7981 gttagaactc aggaccaact tattctgatt ttgtttttcc aaactgcttc tcctcttggg 8041 aagtgtaagg aagctgcagc accaggatca gtgaaacgca ccagacagcc gcgtcagagc 8101 agctcaggtt ctgggagagg gtagcgcagg gtggccactg agaaccgggc aggtcacgca 8161 tccccccctt ccctcccacc ccctgccaag ctctccctcc caggatcctc tctggctcca 8221 tcgtaagcaa accttagagg ttctggcaag gagagagatg gctccaggaa atgggggtgt 8281 gtcaccagat aaggaatctg cctaacagga ggtgggggtt agacccaata tcaggagact 8341 aggaaggagg aggcctaagg atggggcttt tctgtcacca atcctgtccc tagtagcggc 8401 cgcgaagttc ctatactttc tagagaatag gaacttcttc gaaccataga gcccaccgca 8461 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca 8521 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag 8581 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca 8641 acagatggct ggcaactaga aggcacagtc gaggcactag tttacccggg gagcatgtca 8701 aggtcaaaat cgtcaagagc gtcagcaggc agcatatcaa ggtcaaagtc gtcaagggca 8761 tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt cggccggccc gccgctttcg 8821 cactttagct gtttctccag gccacatatg attagttcca ggccgaaaag gaaggcaggt 8881 tcggctccct gccggtcgaa cagctcaatt gcttgtctca gaagtggggg catagaatcg 8941 gtggtaggtg tctctctttc ctcttttgct acttgatgct cctgttcctc caatacgcag 9001 cccagtgtaa agtggcccac ggcggacaga gcgtacagtg cgttctccag ggagaagcct 9061 tgctgacaca ggaacgcgag ctgattttcc agggtttcgt actgtttctc tgttgggcgg 9121 gtgccgagat gcactttagc cccgtcgcga tgtgagagga gagcacagcg gaatgacttg 9181 gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca gggggcagaa gtgggtatga 9241 tgcctgtcca gcatctcgat tggcagggca tcgagcaggg cccgcttgtt cttcacgtgc 9301 cagtacaggg taggctgctc aactcccagc ttttgagcga gtttccttgt cgtcaggcct 9361 tcgataccga ctccattgag taattccaga gcgccgttta tgactttgct cttgtccagt 9421 ctagacatga attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg 9481 agctgtagga aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc 9541 acacgccaga agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc 9601 ggacagcccc gaggctggag agggagaagg ggacggcggc gcggcgacgc acgaaggccc 9661 tccccgccca tttccttcct gccggcgccg caccgcttcg ccccgcgccc gctagagggg 9721 gtgcggcggc gcctcccaga tttcggctcc gcacagattt gggacaaagg aagtccctgc 9781 gccctctcgc acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg 9841 ccggcgctcc gggggccgcc gcgcccctcc cccgagccct ccccggcccg aggcggcccc 9901 gccccgcccg gcacccccac ctgccgccac cccccgcccg gcacggcgag ccccgcgcca 9961 cgccccgtac ggagccccgc acccgaagcc gggccgtgct cagcaactcg gggagggggg 10021 tgcagggggg gttgcagccc gaccgacgcg cccacacccc ctgctcaccc ccccacgcac 10081 acaccccgca cgcagccttt gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc 10141 ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag 10201 cgctcacagc cgccgggcag cgcggagccg cacgcggcgc tccccacgca cacacacacg 10261 cacgcacccc ccgagccgct ccccccgcac aaagggccct cccggagccc ctcaaggctt 10321 tcacgcagcc acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga 10381 ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc 10441 gggcggcgcg aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc 10501 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga 10561 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc 10621 cgcccctcgc cccgccccgc cccgcctggc gcgcgccccc cccccccccc cgcccccatc 10681 gctgcacaaa ataattaaaa aataaataaa tacaaaattg ggggtgggga ggggggggag 10741 atggggagag tgaagcagaa cgtggggctc acctcgacca tcatggtaat agcgatgact 10801 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc 10861 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact 10921 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa 10981 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg 11041 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat 11101 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagctag tcaataatca 11161 atgtcgacga tgatgctgta cactcgaaac ttttttcgaa ggtccgaggt tctagacgag 11221 tttac //