LOCUS       Exported                3078 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  dsRNA expression .
ACCESSION   .
VERSION     .
KEYWORDS    U6-Lin28aIR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3078)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(756..996)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     complement(806..825)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(976..996)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1096..1113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1267..1855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1347..1366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2026..2886)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2649..2668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2887..2991)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3059..3077
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag aaaaaaagct tggtttgaca cttgtttcgc ttcccgtctg gcaagggaaa
       61 tatcacacag ccttctacaa aagaacttcc ctagcccagc cacttggcaa agcctcgggt
      121 tctagggtgg ttcctcccca caaaagcctt cacttgcttt cagatagagt gtggtaggag
      181 ttcctttgtc tcatcctttc cttccctcca aggaaagaca gggtgacact gggaaccccg
      241 gcctgagaca cggcagtgca agctacccac gcaacccacc cttaccccca ctttctccac
      301 tctgcagatt gatgcctctt tccttaaact ccccattagc caaagaataa ccctgactcc
      361 tgggcctcaa ttctgggctt ctgggagcag ggcagggctg tggatctctt cctcttcctc
      421 cctggatcca ggagtttaag gaaagaggca tcaatctgca gagtggagaa agtgggggta
      481 agggtgggtt gcgtgggtag cttgcactgc cgtgtctcag gccggggttc ccagtgtcac
      541 cctgtctttc cttggaggga aggaaaggat gagacaaagg aactcctacc acactctatc
      601 tgaaagcaag tgaaggcttt tgtggggagg aaccacccta gaacccgagg ctttgccaag
      661 tggctgggct agggaagttc ttttgtagaa ggctgtgtga tatttccctt gccagacggg
      721 aagcgaaaca agtgtcaaac caagcttggt gtttcgtcct ttccacaaga tatataaagc
      781 caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa
      841 ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa
      901 tatctttgtg tttacagtca aattaattcc aattatctct ctaacagcct tgtatcgtat
      961 atgcaaatat gaaggaatca tgggaaatag gccctcaatt gctttccagt cgggaaacct
     1021 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1081 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1141 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1201 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1261 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1321 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1381 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1441 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1501 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1561 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1621 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1681 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     1741 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1801 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1861 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1921 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1981 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2041 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2101 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2161 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2221 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2281 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2341 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2401 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2461 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2521 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2581 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2641 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2701 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2761 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2821 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2881 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2941 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3001 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3061 taggcgtatc acgaggcc
//
