LOCUS Exported 3196 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION crtE in pStA0. ACCESSION . VERSION . KEYWORDS pStA0::crtE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3196) AUTHORS Taylor GM, Mordaka PM, Heap JT TITLE Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering. JOURNAL Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182. PUBMED 30462270 REFERENCE 2 (bases 1 to 3196) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3196 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 986..1072 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(1085..1103) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1171..1275 /gene="bla" /label=AmpR promoter CDS 1276..2136 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1494..1513) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2307..2895 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2796..2815 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 3053..3147 /label=lambda t0 terminator /note="transcription terminator from phage lambda" ORIGIN 1 tgttaccgga aacctccgcg acgccggaat tggcacgctt ttacgcgctc ctgcgcgact 61 atcctcaacg tggtggcaag ggcattcggt cagaattact gcttgcctct gctcgtgcgc 121 acggcctgtc cgagtcagat accggttggg agtcagcatt atggctggcg gcagccttag 181 aactgtttca gaactgggtg ctggtgcacg atgatattga agatgattcg gaagaacgcc 241 gtggtcgtcc ggccctgcac cacttgtgtg gtatgccggt cgctcttaac gtgggggacg 301 cgctgcacgc ttacatgtgg gctgctgttg ggaaagccaa tgttccggga gcgtttgaag 361 agtttctgca gatggtgtac cgcacggcgg aaggccagca tctggatctg gcatgggtgg 421 agggtcgtga atggggcctg cgtcccgcgg attatctcca gatggttggc ctgaaaaccg 481 cacactacac ggttatcgtg ccgttacgtc tgggggccct ggcggcaggc atggcaccgc 541 aggacgcgtt caccccagcg ggtctggcgc tgggtaccgc gtttcagatt cgtgacgatg 601 tcctcaatct ggcaggtgat ccggtgaagt atggtaaaga aattggtggc gatctgttgg 661 aaggtaaacg tactctgatt gtcctggact ggttgactac ggcgccggat gatcgcaaag 721 ccatcttcct ggaccagatg cgtcggcacc gcgcagataa agaccctgcg gtgatcgatg 781 aaattcaccg ctggctgctt gaaagcggct ctgtggaagc ggcgcaggac tacgcgcagg 841 cacaagccgc ggaaggtctg gacttgcttg aaaaagcatt ggcagacgcg ccggatgccc 901 aggccgccgc tgccttactc gcttctgttc gggaactggc cacccgcgaa aaataatgaa 961 gagcgtaaga cctctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac 1021 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcccga 1081 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 1141 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 1201 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 1261 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 1321 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 1381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 1441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 1501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 1561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 1621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 1681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 1741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 1801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 1861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 1921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 1981 ggtgagcgtg ggtcccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 2041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 2101 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 2161 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 2221 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 2281 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 2341 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 2401 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 2461 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 2521 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 2581 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 2641 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 2701 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 2761 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 2821 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2881 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 2941 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 3001 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcct tggactcctg 3061 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc 3121 cgccgggcgt tttttattgg tgagaatcca gtgtgctctt cgatgcgccc ggaattactc 3181 gcacgcgtgt taagcc //