LOCUS Exported 2244 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION RBS RBSc42 in pStA0. ACCESSION . VERSION . KEYWORDS pStA0::RBSc42 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2244) AUTHORS Taylor GM, Mordaka PM, Heap JT TITLE Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering. JOURNAL Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182. PUBMED 30462270 REFERENCE 2 (bases 1 to 2244) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2244 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 83..169 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(182..200) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 268..372 /gene="bla" /label=AmpR promoter CDS 373..1233 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(591..610) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1404..1992 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1893..1912 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2150..2244 /label=lambda t0 terminator /note="transcription terminator from phage lambda" ORIGIN 1 ccagtgtggc tcttcgccaa caggatacat ctgtaaagga ggtaacgatg atgtgaagag 61 cgtaagacct ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac 121 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcccgaaag 181 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 241 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 301 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 361 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 421 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 481 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 541 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 601 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 661 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 721 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 781 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 841 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 901 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 961 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 1021 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 1081 gagcgtgggt cccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 1141 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 1201 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 1261 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 1321 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 1381 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 1441 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 1501 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 1561 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 1621 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 1681 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 1741 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 1801 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 1861 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 1921 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 1981 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 2041 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 2101 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgccttgg actcctgttg 2161 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc 2221 cgggcgtttt ttattggtga gaat //