LOCUS Exported 2266 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Terminator L3S2P55 in pStA0. ACCESSION . VERSION . KEYWORDS pStA0::L3S2P55 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2266) AUTHORS Taylor GM, Mordaka PM, Heap JT TITLE Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering. JOURNAL Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182. PUBMED 30462270 REFERENCE 2 (bases 1 to 2266) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2266 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..87 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(100..118) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 186..290 /gene="bla" /label=AmpR promoter CDS 291..1151 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(509..528) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1322..1910 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1811..1830 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2068..2162 /label=lambda t0 terminator /note="transcription terminator from phage lambda" ORIGIN 1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 61 tttcgactga gcctttcgtt ttatttgatg cccgaaaggg cctcgtgata cgcctatttt 121 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 181 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 241 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 301 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 361 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 421 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 481 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 541 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 601 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 661 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 721 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 781 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 841 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 901 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 961 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtcc cgcggtatca 1021 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 1081 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 1141 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 1201 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 1261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 1321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 1381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 1441 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 1501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 1561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 1621 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 1681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 1741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 1801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 1861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 1921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 1981 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 2041 gccgcagccg aacgaccgag cgccttggac tcctgttgat agatccagta atgacctcag 2101 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga 2161 atccagtgtg gctcttcata actcggtacc aaagacgaac aataagacgc tgaaaagcgt 2221 cttttttcgt tttggtccgg acgaagagcg acctctaggg cggcgg //