LOCUS       Exported               10014 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  To express membrane targeted tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    pBOB-Lck-tdTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10014)
  TITLE     Cooper Lab plasmids
REFERENCE   2  (bases 1 to 10014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10014
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    219..335
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        358..661
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        662..865
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     862..886
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      942..951
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      1077..1086
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1083..2513
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    2649..3237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2702..2722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3120..3131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3644..3824
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3850..3867)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3856..4080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4126..4554
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4213..4232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4423..4444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4563..4583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4568..4897
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4748..4883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4810..4829
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4945..4992
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5011..5385
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5515..5636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5552..5571)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5606..5625
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5685..5701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5685..5701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5698..5720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5709..5725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5733..5763)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5778..5799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5916..5933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6087..6675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6167..6186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6846..7706)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7469..7488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7707..7811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7886..7905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8077..8456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8458..8656
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8674..8854
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8901..9026
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9519..9752
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9937..9981
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
       61 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
      121 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
      181 gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa aggggggatt
      241 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      301 gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg agttccgcgt
      361 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      421 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      481 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      541 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      601 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      661 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      721 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      781 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      841 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
      901 tccacgctgt tttgacctcc atagaagaca ccgactctag agccaccatg ggctgtggct
      961 gcagctcaaa ccctgaagat gactggatgg agaacattga cgtgtgtgag aactgccatt
     1021 atcccggcgg acgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     1081 ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     1141 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1201 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     1261 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     1321 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1381 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     1441 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     1501 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     1561 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     1621 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     1681 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     1741 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     1801 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     1861 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     1921 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     1981 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     2041 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2101 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     2161 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     2221 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     2281 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     2341 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     2401 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     2461 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag tagagcggcc
     2521 gcgtcgagtt taaacctgga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     2581 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     2641 cctcagacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2701 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2761 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2821 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2881 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2941 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3001 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     3061 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3121 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3181 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     3241 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3301 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     3361 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     3421 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     3481 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     3541 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     3601 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     3661 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3721 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3781 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccg ctttaaaccc
     3841 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3901 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3961 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4021 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4081 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4141 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4201 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4261 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4321 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4381 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4441 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4501 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4561 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4621 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4681 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4741 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4801 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4861 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4921 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     4981 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     5041 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     5101 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     5161 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     5221 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     5281 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     5341 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     5401 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5461 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     5521 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5581 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5641 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     5701 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5761 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5821 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5881 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5941 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6001 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6061 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6961 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7741 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7801 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     7861 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7921 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     7981 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     8041 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     8101 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     8161 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     8221 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     8281 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     8341 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     8401 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     8461 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     8521 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     8581 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     8641 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     8701 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8761 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8821 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8881 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8941 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     9001 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     9061 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     9121 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     9181 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     9241 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     9301 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     9361 aaacggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9421 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9481 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9541 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9601 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9661 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9721 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9781 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9841 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9901 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9961 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtgg
//
