LOCUS Exported 2248 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Promoter J23107 in pStA0. ACCESSION . VERSION . KEYWORDS pStA0::J23107 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2248) AUTHORS Taylor GM, Mordaka PM, Heap JT TITLE Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering. JOURNAL Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182. PUBMED 30462270 REFERENCE 2 (bases 1 to 2248) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2248 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..105 /gene="bla" /label=AmpR promoter CDS 106..966 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(324..343) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1137..1725 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1626..1645 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1883..1977 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 2064..2150 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(2163..2181) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtcccgcgg tatcattgca 841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 1861 agccgaacga ccgagcgcct tggactcctg ttgatagatc cagtaatgac ctcagaactc 1921 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca 1981 gtgtggctct tcgcagttta cggctagctc agccctaggt attatgctag cccatgaaga 2041 gcgtaagacc tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa 2101 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcccgaaa 2161 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac 2221 gtcaggtggc acttttcggg gaaatgtg //