LOCUS Exported 2248 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Promoter J23118 in pStA0. ACCESSION . VERSION . KEYWORDS pStA0::J23118 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2248) AUTHORS Taylor GM, Mordaka PM, Heap JT TITLE Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering. JOURNAL Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182. PUBMED 30462270 REFERENCE 2 (bases 1 to 2248) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2248 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(13..31) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 99..203 /gene="bla" /label=AmpR promoter CDS 204..1064 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(422..441) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1235..1823 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1724..1743 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1981..2075 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 2162..2248 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 atgcccgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 61 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 181 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 421 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 721 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 901 ctggagccgg tgagcgtggg tcccgcggta tcattgcagc actggggcca gatggtaagc 961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 1021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 1081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 1141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 1201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 1261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 1321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 1381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 1441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 1501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 1561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 1621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 1681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 1741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 1801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 1861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 1921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgccttg 1981 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc 2041 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtggctcttc gcagttgacg 2101 gctagctcag tcctaggtat tgtgctagcc catgaagagc gtaagacctc tagggcggcg 2161 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt 2221 ctttcgactg agcctttcgt tttatttg //