LOCUS       Exported               10966 bp ds-DNA     circular SYN 23-SEP-2022
DEFINITION  Backbone to clone single or  paired sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    pDECKO_mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 10966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 23, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10966
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     21..38
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    155..176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        191..221
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    229..245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     234..256
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     253..269
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     253..269
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     288..308
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        290..308
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        336..562
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             563..743
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    790..915
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1408..1641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1826..1870
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2048..2288
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     2048..2068
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2219..2238
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4178..4253
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4309..4426
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4565..5743
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4796..5734
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5691..5711
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5756..6352
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5756..5775)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6252..6272
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6362..6415
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6422..6431
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6428..7138
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6573..6591)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6841..6860
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7154..7742
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7207..7227)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7625..7636)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7814..8047
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8125..8246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8162..8181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8216..8235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8286..8421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8348..8367
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8441..8460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8442..8460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8470..8487)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8470..8486)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8479..8501)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8628..9083
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8715..8734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8925..8946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9109..9213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9214..10074
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9432..9451)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10245..10833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10734..10753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
       61 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      121 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      181 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      241 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
      301 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
      361 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
      421 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
      481 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
      541 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
      601 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      661 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      721 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      781 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      841 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      901 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
      961 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1021 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1081 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1141 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1201 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1261 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1321 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1381 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1441 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1501 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1561 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1621 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1681 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1741 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1801 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1861 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     1921 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     1981 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2041 acccagagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     2101 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     2161 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     2221 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     2281 gaaaggacga aacaccggag acggttgtaa atgagcacac aaaatacaca tgctaaaata
     2341 ttatattcta tgacctttat aaaatcaacc aaaatcttct ttttaataac tttagtatca
     2401 ataattagaa tttttatgtt cctttttgca aacttttaat aaaaatgagc aaaataaaaa
     2461 aacgctagtt ttagtaactc gcgttgtttt cttcaccttt aataatagct actccaccac
     2521 ttgttcctaa gcggtcagct cctgcttcaa tcattttttg agcatcttca aatgttctaa
     2581 ctccaccagc tgctttaact aaagcattgt ctttaacaac tgacttcatt agtttaacat
     2641 cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt tttaacaaat tctaatccag
     2701 cttcaacagc tatttcacaa gctttcatga tttcttcttt tgttaataaa caattttcca
     2761 taatacattt aacaacatgt gatccagctg ctttttttac agctttcatg tcttctaaaa
     2821 ctaattcata atttttgtct tttaatgcac caatatttaa taccatatca atttctgttg
     2881 caccatcttt aattgcttca gaaacttcga atgcttttgt agctgttgtg catgcaccta
     2941 gaggaaaacc tacaacattt gttattccta catttgtgcc ttttaataat tctttacaat
     3001 agcttgttca atatgaatta acacaaactg ttgcaaaatc aaattcaatt gcttcatcac
     3061 ataattgttt aatttcagct ttcgtagcat cttgttttaa taatgtgtga tctatatatt
     3121 tgtttagttt cattttttct cctatatatt catttttaat tttaattctt taataatttc
     3181 gtctacttta actttagcgt tttgaacaga ttcaccaaca cctataaaat aaatttttag
     3241 tttaggttca gttccacttg ggcgaacagc aaatcatgac ttatcttcta aataaaattt
     3301 tagtaagtct tgtcctggca tattatacat tccatcgatg tagtcttcaa cattaacaac
     3361 tttaagtcca gcaatttgag ttaagggtgt tgctctcaat gatttcatta atggttcaat
     3421 ttttaatttc ttttcttctg gtttaaaatt caagtttaaa gtgaaagtgt aatatgcacc
     3481 catttcttta aataaatctt ctaaatagtc tactaatgtt ttattttgtt ttttataaaa
     3541 tcaagcagcc tctgctatta atatagaagc ttgtattcca tctttatctc tagctgagtc
     3601 atcaattaca tatccataac tttcttcata agcaaaaaca aaatttaatc cgttatcttc
     3661 ttctttagca atttctctac ccattcattt aaatccagtt aaagttttta caatattaac
     3721 tccatatttt tcatgagcga ttctatcacc caaatcactt gttacaaaac ttgaatatag
     3781 agccggattt tttggaatgc tatttaagcg ttttagattt gataattttc aatcaattaa
     3841 aattggtcct gtttgatttc catctaatct tacaaaatga ccatcatgtt ttattgccat
     3901 tccaaatctg tcagcatctg ggtcattcat aataataata tctgcatcat gtttaatacc
     3961 atattcaagc ggtatttttc atgcaggatc aaattctgga tttggattta caacattttt
     4021 aaatgtttca tcttcaaatg catgctcttc aacctcaata acgttatatc ctgattcacg
     4081 taatattttt ggggtaaatt tagttcctgt tccattaact gcgctaaaaa taatttttaa
     4141 atctttttta gcttcttgct cttttttgta cgtctctgtt ttagagctag aaatagcaag
     4201 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttta
     4261 agcttggcgt aactagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     4321 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     4381 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     4441 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa
     4501 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     4561 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4621 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     4681 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     4741 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     4801 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     4861 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     4921 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     4981 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     5041 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     5101 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     5161 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     5221 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5281 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5341 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     5401 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5461 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5521 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5581 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5641 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5701 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg ccaccatgac
     5761 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     5821 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     5881 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     5941 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     6001 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6061 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6121 gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6181 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6241 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6301 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccggatcggg
     6361 agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaccggt
     6421 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     6481 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     6541 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     6601 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     6661 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     6721 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     6781 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     6841 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     6901 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     6961 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     7021 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     7081 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaac
     7141 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7201 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7261 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7321 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7381 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7441 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7501 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7561 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7621 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7681 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7741 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7801 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     7861 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7921 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7981 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8041 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8101 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     8161 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8221 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8281 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8341 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8401 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     8461 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8521 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8581 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8641 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8701 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8761 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8821 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8881 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8941 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9001 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     9061 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     9121 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9181 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9241 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9301 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9361 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9421 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9481 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9541 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9601 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9661 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9721 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9781 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9841 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9901 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9961 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10021 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10081 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10141 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10201 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10261 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10321 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10381 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10441 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10501 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10561 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10621 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10681 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10741 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10801 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10861 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10921 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgcc
//
