LOCUS Exported 9949 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION when used to produce lentivirus, express physiological levels of insert . ACCESSION . VERSION . KEYWORDS beta-catenin (S33Y)-pcw107 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9949) AUTHORS Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN, Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, McDonnell DP, Sabatini DM, Wood KC TITLE Systematic identification of signaling pathways with potential to confer anticancer drug resistance. JOURNAL Sci Signal. 2014 Dec 23;7(357):ra121. doi: 10.1126/scisignal.aaa1877. PUBMED 25538079 REFERENCE 2 (bases 1 to 9949) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9949 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 98..115 /label=L4440 /note="L4440 vector, forward primer" protein_bind 232..253 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 268..298 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 306..322 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 311..333 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 330..346 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 330..346 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 365..385 /label=T3 /note="T3 promoter, forward primer" promoter 367..385 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 413..639 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 640..820 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 867..992 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1485..1718 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1903..1947 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 2178..2388 /label=SV40 promoter /note="SV40 early promoter" promoter 2200..2396 /label=SV40 promoter /note="SV40 early promoter" rep_origin 2247..2382 /label=SV40 ori /note="SV40 origin of replication" CDS 2428..3030 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(2428..2447) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 2924..2944 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 3073..3190 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 3248..3748 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 3667..3685 /label=hPGK-F /note="Human PGK promoter, forward primer" protein_bind 3769..3793 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3811..6156 /codon_start=1 /gene="CTNNB1" /product="protein involved both in cell-cell adhesion as part of the cadherin protein complex, and in gene transcription as part of the Wnt signaling pathway" /label=CTNNB1 /note="also known as catenin beta-1; related to Drosophila armadillo" /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDYGIHSGATTTAPS LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL PDLGHAQDLMDGLPPGDSNQLAWFDTDL" protein_bind complement(6158..6182) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 6214..6802 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6267..6287) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6685..6696) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6874..7107 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7185..7306 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7222..7241) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7276..7295 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7346..7481 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(7501..7520) /label=T7 /note="T7 promoter, forward primer" promoter complement(7502..7520) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7530..7547) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7530..7546) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7539..7561) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7688..8143 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7775..7794) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7985..8006 /label=F1ori-F /note="F1 origin, forward primer" promoter 8169..8273 /gene="bla" /label=AmpR promoter CDS 8274..9134 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8492..8511) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9305..9893 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9794..9813 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct 421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa 481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta 541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc 601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag 661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga 1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag 1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt 1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag 1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag 1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc 1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga 1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa 1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc 1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta 1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg 1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct 2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac 2101 ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc aggtgtggaa 2161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa 2221 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 2281 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 2341 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 2401 tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc 2461 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 2521 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 2581 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 2641 gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 2701 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 2761 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 2821 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 2881 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 2941 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 3001 tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac aaatggcagt 3061 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat 3121 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat 3181 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggc 3241 ctgcaggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg 3301 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt 3361 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc 3421 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga 3481 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg 3541 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg 3601 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc 3661 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt 3721 tgaccgaatc accgacctct ctccccaggg gctagcatcg atggatcaac aagtttgtac 3781 aaaaaagttg gctcaggcgg cggcggcacc atggctactc aagctgattt gatggagttg 3841 gacatggcca tggaaccaga cagaaaagcg gctgttagtc actggcagca acagtcttac 3901 ctggactatg gaatccattc tggtgccact accacagctc cttctctgag tggtaaaggc 3961 aatcctgagg aagaggatgt ggatacctcc caagtcctgt atgagtggga acagggattt 4021 tctcagtcct tcactcaaga acaagtagct gatattgatg gacagtatgc aatgactcga 4081 gctcagaggg tacgagctgc tatgttccct gagacattag atgagggcat gcagatccca 4141 tctacacagt ttgatgctgc tcatcccact aatgtccagc gtttggctga accatcacag 4201 atgctgaaac atgcagttgt aaacttgatt aactatcaag atgatgcaga acttgccaca 4261 cgtgcaatcc ctgaactgac aaaactgcta aatgacgagg accaggtggt ggttaataag 4321 gctgcagtta tggtccatca gctttctaaa aaggaagctt ccagacacgc tatcatgcgt 4381 tctcctcaga tggtgtctgc tattgtacgt accatgcaga atacaaatga tgtagaaaca 4441 gctcgttgta ccgctgggac cttgcataac ctttcccatc atcgtgaggg cttactggcc 4501 atctttaagt ctggaggcat tcctgccctg gtgaaaatgc ttggttcacc agtggattct 4561 gtgttgtttt atgccattac aactctccac aaccttttat tacatcaaga aggagctaaa 4621 atggcagtgc gtttagctgg tgggctgcag aaaatggttg ccttgctcaa caaaacaaat 4681 gttaaattct tggctattac gacagactgc cttcaaattt tagcttatgg caaccaagaa 4741 agcaagctca tcatactggc tagtggtgga ccccaagctt tagtaaatat aatgaggacc 4801 tatacttacg aaaaactact gtggaccaca agcagagtgc tgaaggtgct atctgtctgc 4861 tctagtaata agccggctat tgtagaagct ggtggaatgc aagctttagg acttcacctg 4921 acagatccaa gtcaacgtct tgttcagaac tgtctttgga ctctcaggaa tctttcagat 4981 gctgcaacta aacaggaagg gatggaaggt ctccttggga ctcttgttca gcttctgggt 5041 tcagatgata taaatgtggt cacctgtgca gctggaattc tttctaacct cacttgcaat 5101 aattataaga acaagatgat ggtctgccaa gtgggtggta tagaggctct tgtgcgtact 5161 gtccttcggg ctggtgacag ggaagacatc actgagcctg ccatctgtgc tcttcgtcat 5221 ctgaccagcc gacaccaaga agcagagatg gcccagaatg cagttcgcct tcactatgga 5281 ctaccagttg tggttaagct cttacaccca ccatcccact ggcctctgat aaaggctact 5341 gttggattga ttcgaaatct tgccctttgt cccgcaaatc atgcaccttt gcgtgagcag 5401 ggtgccattc cacgactagt tcagttgctt gttcgtgcac atcaggatac ccagcgccgt 5461 acgtccatgg gtgggacaca gcagcaattt gtggaggggg tccgcatgga agaaatagtt 5521 gaaggttgta ccggagccct tcacatccta gctcgggatg ttcacaaccg aattgttatc 5581 agaggactaa ataccattcc attgtttgtg cagctgcttt attctcccat tgaaaacatc 5641 caaagagtag ctgcaggggt cctctgtgaa cttgctcagg acaaggaagc tgcagaagct 5701 attgaagctg agggagccac agctcctctg acagagttac ttcactctag gaatgaaggt 5761 gtggcgacat atgcagctgc tgttttgttc cgaatgtctg aggacaagcc acaagattac 5821 aagaaacggc tttcagttga gctgaccagc tctctcttca gaacagagcc aatggcttgg 5881 aatgagactg ctgatcttgg acttgatatt ggtgcccagg gagaacccct tggatatcgc 5941 caggatgatc ctagctatcg ttcttttcac tctggtggat atggccagga tgccttgggt 6001 atggacccca tgatggaaca tgagatgggt ggccaccacc ctggtgctga ctatccagtt 6061 gatgggctgc cagatctggg gcatgcccag gacctcatgg atgggctgcc tccaggtgac 6121 agcaatcagc tggcctggtt tgatactgac ctgtaattgc caactttctt gtacaaagtg 6181 gttaccggtt agtaatgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt 6241 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 6301 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 6361 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 6421 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 6481 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 6541 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6601 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc 6661 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6721 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 6781 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag 6841 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 6901 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 6961 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 7021 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 7081 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc 7141 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag 7201 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 7261 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct 7321 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 7381 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 7441 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg 7501 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg 7561 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 7621 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 7681 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 7741 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 7801 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 7861 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 7921 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 7981 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 8041 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 8101 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg 8161 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 8221 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 8281 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 8341 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 8401 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 8461 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 8521 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 8581 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 8641 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 8701 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 8761 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 8821 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 8881 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 8941 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 9001 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 9061 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 9121 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 9181 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 9241 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 9301 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 9361 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 9421 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 9481 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 9541 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 9601 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 9661 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 9721 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 9781 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 9841 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 9901 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctca //