LOCUS Exported 11477 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes . ACCESSION . VERSION . KEYWORDS MiniCoopR mitfa:EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11477) AUTHORS Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I TITLE Human tumor genomics and zebrafish modeling identify SPRED1 loss as a driver of mucosal melanoma. JOURNAL Science. 2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509. PUBMED 30385465 REFERENCE 2 (bases 1 to 11477) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11477 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(144..165) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1061..1077 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 1097..1117 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" protein_bind 2991..3015 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3021..3737 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3021..3737 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3066..3087) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3327..3346) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3674..3695 /label=EGFP-C /note="EGFP, forward primer" protein_bind complement(3799..3823) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" polyA_signal 3825..3959 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind complement(4022..4042) /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(4050..4066) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" polyA_signal 4082..4216 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9240..9256) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9240..9256) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(9293..9313) /label=T3 /note="T3 promoter, forward primer" promoter complement(9293..9311) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(9332..9348) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9345..9367) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9356..9372 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9380..9410) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9425..9446 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9563..9580) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9734..10322) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9814..9833) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10493..11353) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11116..11135 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11354..11458) /gene="bla" /label=AmpR promoter ORIGIN 1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa 241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg 301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca 361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa 421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc 481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt 541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat 601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt 661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa 721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta 781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta 841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc 901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc 961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga 1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat 1081 gattacgcca agctatcaac tttgtataga aaagttgtga gtgctaacaa ctgggctgct 1141 gtgccgctct ttcgaggaaa tgaaaggaac tctggcggcc tatgatgagt taggaattgt 1201 ctgattggtg aatcatacat aggctaatgt gggaccagct gtagtcagtc ataagcacgt 1261 gattctctcg aaattagttt ataaataaac ttcactaaac tccaaccgtt cattctgttt 1321 tagatgttgc ctttaaactt gccttaagat gttaataaag ttgctttttg gttgttgaca 1381 aagctaggca taggacgata accgttttca aggtataccc cggtttgaaa aagtcaaggg 1441 tttaaaacca ccaatatttt ctgctatacc gttcctaagg tatatttaag attttttatt 1501 tattttttat ttttttgttt tttaggacaa cagcattttc agcagaaaag agatacaaaa 1561 atgcttttta aattgtaaag aaatctgttt ttgaaactaa tgaagacagc agaagtcgat 1621 cattcattga aattattaag cttgacatgt tttctgttcc aaaatatttt ttcaatgttt 1681 ctcaaaataa aatatattat gtttaaagtt tttgtttttt actaagacat taaaaaaaat 1741 aatatatttt agagtggcaa tcacaatacc atcaaactgt gatattttta tcaaaggtta 1801 tcataccatc agaatcttat accccatgcc tagacacagc tgttctctct atctctctat 1861 gttaatgcag tttccactcc attgacgaga tggttctctg taccacatac tcatccccat 1921 tttacaatag ctacatttgc tcaagatgta gtcaactcaa acaatgcaga gacaacaaac 1981 tgttgactta tcttttctat cgtaattcag cccttttggg aaatgagcat caacacacaa 2041 tttagagtgg aagttgggtt aaggcagact cattttttac acattacaac cagtgattat 2101 gtcaattatt atttaaaaaa aattcagtac agtatatgca catgctactt tgtttatgat 2161 tgttagcttt tttaactaca gttcatttct atgctttctg tgcaaatgca caacagttac 2221 tgatttactc tgatgataat tgtgccagta catccaacaa caacaaaaaa aaaaaaaaga 2281 cttacaatca agccccaaag tgttcatacg tctggcaaat tctgacttaa agttactttt 2341 attcaaccag ctagtttttt tttgactgta aatgacacgt aagataataa gatgatgtac 2401 atgaggcatc attgtagaaa aaaaaatgaa tatgaatctg aatatgaact gtacgtctgt 2461 gattttgttt gtgcgcactt acatacgtgt gcacaatatt tcaactctta agaaaaaaat 2521 gcaattacca aaggcccatc agacaacaaa gttctctttc ctatcttttg ctcttcaaca 2581 tttcagagat aactccatct taaactgtct tgtttttaga ccaacagtgc tagtgattgt 2641 atgccggatg ctgagaacaa acaatgtttt atgcaggatg agtggaacta aagatgaaac 2701 gttagttagc cgtaagccag tttcagttta gacactagta acccatcgtc tcaaagaggc 2761 ttttgtcgaa tcggaccggt gacgtcaaat gaactttata aaggcataaa ttcatcgttt 2821 gggtaaaaaa aacaatatga gaagagcaat cccctcccac ccccttaaat acattttgtg 2881 tcttttcttt ggcattgaca tcttcagctg gccaagacga ctggtcagtt cttgcacatc 2941 acctaaattc acaaagggaa tacttttttg aagctaacac atagttgaac gcaagtttgt 3001 acaaaaaagc aggctggacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 3061 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 3121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 3181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 3241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 3301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 3361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 3421 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 3481 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 3541 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 3601 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 3661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 3721 tggacgagct gtacaagggc ggtggaagat ctgggaattc aaggcctctc gagcctctag 3781 attctgcagc cctatagcac ccagctttct tgtacaaagt gggggatcca gacatgataa 3841 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 3901 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 3961 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt 4021 ccaactttat tatacatagt tgataattca ctggccgtcg ttttacggta ccatcgatga 4081 tgatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 4141 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 4201 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 4261 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga 4321 tcctctagat cagatctcag attgtatcca aaatatcatt ttttgtgttt taaagatgaa 4381 caaaggtctc agggaattgg aacgacatga ggatgattaa ttaataaaaa aaaattcatt 4441 tttgggtgaa ctatccctaa aatcaaagca atttgggcat tttttttcta attattatgg 4501 agaacttgca tcacagctct tccactagaa aataaaacaa atttcagaaa tactgcagaa 4561 atacttttgg acttaatgtt aaataacaat gtaaaaagtt tttccagggc atcgtggtgg 4621 tgcagtgggt agcattgtcg ccttacagca agaaggtcgc tggttcgaga cttggttggg 4681 tcatttctgt gtggagtttg catgttctcc ccatgttcgt gtgggtttcc tccgggtgct 4741 ccagtttcac ctacagccca aagacatgcg ctataggtga attgggtaag ctaaattgtc 4801 taaagtgaat ggtactgtgg tgggtgtttc ccagtgatgg gttgcagctg caagggcatc 4861 cgctggattt gtttgttggc ataagttggc agttcattca actgtggcaa cccctgatta 4921 ataaaggggc taagccaaaa agaaaatgaa tgactgaatg aaagtgaatc cagttgtaca 4981 tgcctagaca tgtcctccac ccttgcaaga tgtacacccc aagaaatgga gctgcatttg 5041 aggacaacaa taagccaagc ctgcaaataa aagacacctc aacagttttt attcaagcta 5101 ctctttatat catccgcgag aatccttcaa aaatcaacta attgtttaca cgatttcatt 5161 cttgactaga ctctgacatg ataaatgaaa aaatatatac acaagcacac taaatattct 5221 gaaatacaag attaatactt tacgaaattg cgctcttcat gatctggatg taacactggt 5281 acttacaaga cacacacaca cacaaatcta attgtctaac tttggttcat gaaatttagt 5341 tggcattgct aacagccatt gtcatgttcg tccatactgc tgctgccgct actgttggaa 5401 ggtactgggg atccggagga caacagcggg tcgctgctgc ccaccggaga cagggtgttg 5461 tccataagca tgtcctccag cttggtggag gccttgtttg ggctgtcgta gacccccggc 5521 tcgccgtcct ccgtgggact gtcattgtag ctgatggtgc cgttgttgag gtccagagtg 5581 gtaggacggg acatgtcagg actgggaagg tgtgggtaca aattggatgt gcagtccccc 5641 atcccaggct cttgtttgat tgctcgagcc actaactcag cggaataaag gctggaagaa 5701 gctacaacgg tgagtccatg agcacgggct tgcatttcaa gttcctgaat acggagcagg 5761 agatgtctgt ttgcgtgttc tagtctcttc tgtctgtttt ccagctcttt tgctttctgc 5821 tgctctttct gcaatttcct aatgtaatcc actgatgctt tcaggatggt gcctttattc 5881 cacctcatgt ctggatcatt tgacttggga attaaagtcc ccagctcctt aattctgtca 5941 ttgatattaa accgccttct tcgttcaatg aggttgtggt tgtccttctt ttgtctctct 6001 tttgccaggg ctctgacttc tgcttctact ggaaagccat caggtctttg ataagagtca 6061 aacttgccac atggtccagc tttgtccatt atgtgcatca tgcccggaga tggagtaacg 6121 gataattccc ttttgacggc cggcaggctt gaggggcagg agttactgat ggaaactcca 6181 gctggaggaa gagcatgatt gctgtacatg tccagcaggt ttgctgaaac tggaatcgtg 6241 tttgtcattt gaagtcctgc atccatgaac ccaagaatgt catcactgta gcttgattcc 6301 aaacttataa tgtcttcaat tacatcgtcc atctctttct cacagttgag ggtgagaagg 6361 gccatagggc tgttgggagc gctggctccg ggtcccgggg tcatgccgtg ctcggcgggc 6421 tggctggggc cgggccctgt gctggcctgt gggctcagct tggctcccag tgcactggac 6481 aggtagtgct tcacctgctg cctctggctc tgctggatgt ggtacttcga gggggtttcc 6541 aggtgggtct gaacctggta gtgactgtac tcgagcatct ccaacatgtt caactatgtg 6601 ttagcttcaa aaaagtattc cctttgtgaa tttaggtgat gtgcaagaac tgaccagtcg 6661 tcttggccag ctgaagatgt caatgccaaa gaaaagacac aaaatgtatt taagggggtg 6721 ggaggggatt gctcttctca tattgttttt tttacccaaa cgatgaattt atgcctttat 6781 aaagttcatt tgacgtcacc ggtccgattc gacaaaagcc tctttgagac gatgggttac 6841 tagtgtctaa actgaaactg gcttacggct aactaacgtt tcatctttag ttccactcat 6901 cctgcataaa acattgtttg ttctcagcat ccggcataca atcactagca ctgttggtct 6961 aaaaacaaga cagtttaaga tggagttatc tctgaaatgt tgaagagcaa aagataggaa 7021 agagaacttt gttgtctgat gggcctttgg taattgcatt tttttcttaa gagttgaaat 7081 attgtgcaca cgtatgtaag tgcgcacaaa caaaatcaca gacgtacagt tcatattcag 7141 attcatattc attttttttt ctacaatgat gcctcatgta catcatctta ttatcttacg 7201 tgtcatttac agtcaaaaaa aaactagctg gttgaataaa agtaacttta agtcagaatt 7261 tgccagacgt atgaacactt tggggcttga ttgtaagtct tttttttttt tttgttgttg 7321 ttggatgtac tggcacaatt atcatcagag taaatcagta actgttgtgc atttgcacag 7381 aaagcataga aatgaactgt agttaaaaaa gctaacaatc ataaacaaag tagcatgtgc 7441 atatactgta ctgaattttt tttaaataat aattgacata atcactggtt gtaatgtgta 7501 aaaaatgagt ctgccttaac ccaacttcca ctctaaattg tgtgttgatg ctcatttccc 7561 aaaagggctg aattacgata gaaaagataa gtcaacagtt tgttgtctct gcattgtttg 7621 agttgactac atcttgagca aatgtagcta ttgtaaaatg gggatgagta tgtggtacag 7681 agaaccatct cgtcaatgga gtggaaactg cattaacata gagagataga gagaacagct 7741 gtgtctaggc atggggtata agattctgat ggtatgataa cctttgataa aaatatcaca 7801 gtttgatggt attgtgattg ccactctaaa atatattatt ttttttaatg tcttagtaaa 7861 aaacaaaaac tttaaacata atatatttta ttttgagaaa cattgaaaaa atattttgga 7921 acagaaaaca tgtcaagctt aataatttca atgaatgatc gacttctgct gtcttcatta 7981 gtttcaaaaa cagatttctt tacaatttaa aaagcatttt tgtatctctt ttctgctgaa 8041 aatgctgttg tcctaaaaaa caaaaaaata aaaaataaat aaaaaatctt aaatatacct 8101 taggaacggt atagcagaaa atattggtgg ttttaaaccc ttgacttttt caaaccgggg 8161 tataccttga aaacggttat cgtcctatgc ctagctttgt caacaaccaa aaagcaactt 8221 tattaacatc ttaaggcaag tttaaaggca acatctaaaa cagaatgaac ggttggagtt 8281 tagtgaagtt tatttataaa ctaatttcga gagaatcacg tgcttatgac tgactacagc 8341 tggtcccaca ttagcctatg tatgattcac caatcagaca attcctaact catcataggc 8401 cgccagagtt cctttcattt cctcgaaaga gcggcacagc agcccagttg ttagcactgt 8461 tgcctcaaag caagaacgtc actggtccaa gtctctaaca ggccagtcca tggttctgtg 8521 tggagtttac atgttgtcct cgtgctcatg tggatatccc ctgggttctc cggttttctc 8581 ctacagtcca aaaacttgcg acataggtga attgaacaaa accaaattag catagacaag 8641 ctcctaatca gcagaatatc tctccgtagc aatctcttat tcgagatctg cgaagatacg 8701 gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg 8761 aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca 8821 ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa 8881 tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa 8941 catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat 9001 agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt 9061 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta 9121 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa 9181 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag 9241 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt 9301 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 9361 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 9421 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 9481 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 9541 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 9601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 9661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 9721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 9781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 9841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 9901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 9961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 10021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 10081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 10141 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 10201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 10261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 10321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 10381 aaggcatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 10441 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 10501 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 10561 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 10621 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 10681 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 10741 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 10801 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 10861 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 10921 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 10981 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 11041 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 11101 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 11161 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 11221 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 11281 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 11341 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 11401 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 11461 catttccccg aaaagtg //