LOCUS Exported 12256 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytes . ACCESSION . VERSION . KEYWORDS MiniCoopR mitfa:SPRED1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12256) AUTHORS Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I TITLE Human tumor genomics and zebrafish modeling identify SPRED1 loss as a driver of mucosal melanoma. JOURNAL Science. 2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509. PUBMED 30385465 REFERENCE 2 (bases 1 to 12256) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12256 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(144..165) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1061..1077 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 1097..1117 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" protein_bind 2991..3015 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind 3016..3033 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3017..3033 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3043..3062 /label=T7 /note="T7 promoter, forward primer" promoter 3043..3061 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4478..4494) /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(4531..4549) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" protein_bind complement(4578..4602) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" polyA_signal 4604..4738 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind complement(4801..4821) /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(4829..4845) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" polyA_signal 4861..4995 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10019..10035) /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(10072..10090) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(10111..10127) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10124..10146) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10135..10151 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10159..10189) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10204..10225 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10342..10359) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10513..11101) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10593..10612) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11272..12132) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11895..11914 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12133..12237) /gene="bla" /label=AmpR promoter ORIGIN 1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa 241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg 301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca 361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa 421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc 481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt 541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat 601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt 661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa 721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta 781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta 841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc 901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc 961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga 1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat 1081 gattacgcca agctatcaac tttgtataga aaagttgtga gtgctaacaa ctgggctgct 1141 gtgccgctct ttcgaggaaa tgaaaggaac tctggcggcc tatgatgagt taggaattgt 1201 ctgattggtg aatcatacat aggctaatgt gggaccagct gtagtcagtc ataagcacgt 1261 gattctctcg aaattagttt ataaataaac ttcactaaac tccaaccgtt cattctgttt 1321 tagatgttgc ctttaaactt gccttaagat gttaataaag ttgctttttg gttgttgaca 1381 aagctaggca taggacgata accgttttca aggtataccc cggtttgaaa aagtcaaggg 1441 tttaaaacca ccaatatttt ctgctatacc gttcctaagg tatatttaag attttttatt 1501 tattttttat ttttttgttt tttaggacaa cagcattttc agcagaaaag agatacaaaa 1561 atgcttttta aattgtaaag aaatctgttt ttgaaactaa tgaagacagc agaagtcgat 1621 cattcattga aattattaag cttgacatgt tttctgttcc aaaatatttt ttcaatgttt 1681 ctcaaaataa aatatattat gtttaaagtt tttgtttttt actaagacat taaaaaaaat 1741 aatatatttt agagtggcaa tcacaatacc atcaaactgt gatattttta tcaaaggtta 1801 tcataccatc agaatcttat accccatgcc tagacacagc tgttctctct atctctctat 1861 gttaatgcag tttccactcc attgacgaga tggttctctg taccacatac tcatccccat 1921 tttacaatag ctacatttgc tcaagatgta gtcaactcaa acaatgcaga gacaacaaac 1981 tgttgactta tcttttctat cgtaattcag cccttttggg aaatgagcat caacacacaa 2041 tttagagtgg aagttgggtt aaggcagact cattttttac acattacaac cagtgattat 2101 gtcaattatt atttaaaaaa aattcagtac agtatatgca catgctactt tgtttatgat 2161 tgttagcttt tttaactaca gttcatttct atgctttctg tgcaaatgca caacagttac 2221 tgatttactc tgatgataat tgtgccagta catccaacaa caacaaaaaa aaaaaaaaga 2281 cttacaatca agccccaaag tgttcatacg tctggcaaat tctgacttaa agttactttt 2341 attcaaccag ctagtttttt tttgactgta aatgacacgt aagataataa gatgatgtac 2401 atgaggcatc attgtagaaa aaaaaatgaa tatgaatctg aatatgaact gtacgtctgt 2461 gattttgttt gtgcgcactt acatacgtgt gcacaatatt tcaactctta agaaaaaaat 2521 gcaattacca aaggcccatc agacaacaaa gttctctttc ctatcttttg ctcttcaaca 2581 tttcagagat aactccatct taaactgtct tgtttttaga ccaacagtgc tagtgattgt 2641 atgccggatg ctgagaacaa acaatgtttt atgcaggatg agtggaacta aagatgaaac 2701 gttagttagc cgtaagccag tttcagttta gacactagta acccatcgtc tcaaagaggc 2761 ttttgtcgaa tcggaccggt gacgtcaaat gaactttata aaggcataaa ttcatcgttt 2821 gggtaaaaaa aacaatatga gaagagcaat cccctcccac ccccttaaat acattttgtg 2881 tcttttcttt ggcattgaca tcttcagctg gccaagacga ctggtcagtt cttgcacatc 2941 acctaaattc acaaagggaa tacttttttg aagctaacac atagttgaac gcaagtttgt 3001 acaaaaaagc aggcttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag 3061 ggcgaattgg gtaccgggcc ccccctcgag gtcgacgcca ccatgagcga ggagacggcg 3121 acttctgaca acgataatag ttatgcacga gtgcgagctg tggtgatgac ccgagatgac 3181 tcaagtggtg gatggttacc acttggaggg agtggactaa gcagcgtcac tgtcttcaaa 3241 gtccctcatc aggaagagaa tggctgtgct gactttttta tccgtggaga gcgactcagg 3301 gacaaaatgg tggttttgga atgtatgctt aaaaaagacc tcatttataa taaggtcact 3361 ccaacatttc accactggaa gattgatgac aaaaagtttg gtcttacgtt tcaaagtcct 3421 gctgatgcta gggcttttga tagaggtatc cgaagagcta tagaggatat ttctcaagga 3481 tgccccgaat caaaaaatga agctgaaggg gcagatgact tacaagcaaa tgaagaggat 3541 tcttccagtt ctctagtgaa ggatcacctt tttcagcaag agacagttgt taccagtgag 3601 ccttatagaa gctcaaatat aagaccttct ccctttgaag atctgaatgc cagaagagtc 3661 tacatgcaaa gccaagccaa tcagataaca tttggtcagc caggcttgga cattcagagc 3721 agaagtatgg aatacgtaca gcggcaaata tccaaggaat gtggaagcct aaagtcccaa 3781 aatagggtcc ctttgaaatc aatcagacat gtcagctttc aagatgagga tgagattgtc 3841 agaataaacc ctcgagatat cttaatacgt cgctatgcag actacagaca tcctgacatg 3901 tggaaaaatg acttggaaag agatgatgct gattccagta ttcagttttc taaaccagac 3961 agtaaaaaat cagactatct gtactcttgt ggggatgaga ctaagttaag ttcacccaaa 4021 gactctgtgg tatttaagac gcagccttcc tcattaaaaa ttaagaagtc aaaacgaaga 4081 aaagaggatg gtgaacgttc tcgctgcgta tactgccagg aaaggtttaa tcatgaagaa 4141 aatgtcaggg gaaaatgtca ggatgctcca gaccctatta aaagatgcat atatcaagtt 4201 agttgcatgc tctgtgcaga gagcatgttg tatcattgta tgtcagactc agagggagat 4261 ttttctgatc cctgttcgtg tgacactagc gacgacaagt tctgcttgcg atggttagcc 4321 ctggtagctt tgtctttcat tgtaccatgt atgtgctgct acgtcccttt gagaatgtgc 4381 catcgctgtg gtgaggcatg tggttgctgt ggtgggaaac ataaagctgc tggattgtaa 4441 atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc tagagcggcc 4501 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc 4561 gtaatcatgg tcatagcacc cagctttctt gtacaaagtg ggggatccag acatgataag 4621 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg 4681 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa 4741 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttc 4801 caactttatt atacatagtt gataattcac tggccgtcgt tttacggtac catcgatgat 4861 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga 4921 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc 4981 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag 5041 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat 5101 cctctagatc agatctcaga ttgtatccaa aatatcattt tttgtgtttt aaagatgaac 5161 aaaggtctca gggaattgga acgacatgag gatgattaat taataaaaaa aaattcattt 5221 ttgggtgaac tatccctaaa atcaaagcaa tttgggcatt ttttttctaa ttattatgga 5281 gaacttgcat cacagctctt ccactagaaa ataaaacaaa tttcagaaat actgcagaaa 5341 tacttttgga cttaatgtta aataacaatg taaaaagttt ttccagggca tcgtggtggt 5401 gcagtgggta gcattgtcgc cttacagcaa gaaggtcgct ggttcgagac ttggttgggt 5461 catttctgtg tggagtttgc atgttctccc catgttcgtg tgggtttcct ccgggtgctc 5521 cagtttcacc tacagcccaa agacatgcgc tataggtgaa ttgggtaagc taaattgtct 5581 aaagtgaatg gtactgtggt gggtgtttcc cagtgatggg ttgcagctgc aagggcatcc 5641 gctggatttg tttgttggca taagttggca gttcattcaa ctgtggcaac ccctgattaa 5701 taaaggggct aagccaaaaa gaaaatgaat gactgaatga aagtgaatcc agttgtacat 5761 gcctagacat gtcctccacc cttgcaagat gtacacccca agaaatggag ctgcatttga 5821 ggacaacaat aagccaagcc tgcaaataaa agacacctca acagttttta ttcaagctac 5881 tctttatatc atccgcgaga atccttcaaa aatcaactaa ttgtttacac gatttcattc 5941 ttgactagac tctgacatga taaatgaaaa aatatataca caagcacact aaatattctg 6001 aaatacaaga ttaatacttt acgaaattgc gctcttcatg atctggatgt aacactggta 6061 cttacaagac acacacacac acaaatctaa ttgtctaact ttggttcatg aaatttagtt 6121 ggcattgcta acagccattg tcatgttcgt ccatactgct gctgccgcta ctgttggaag 6181 gtactgggga tccggaggac aacagcgggt cgctgctgcc caccggagac agggtgttgt 6241 ccataagcat gtcctccagc ttggtggagg ccttgtttgg gctgtcgtag acccccggct 6301 cgccgtcctc cgtgggactg tcattgtagc tgatggtgcc gttgttgagg tccagagtgg 6361 taggacggga catgtcagga ctgggaaggt gtgggtacaa attggatgtg cagtccccca 6421 tcccaggctc ttgtttgatt gctcgagcca ctaactcagc ggaataaagg ctggaagaag 6481 ctacaacggt gagtccatga gcacgggctt gcatttcaag ttcctgaata cggagcagga 6541 gatgtctgtt tgcgtgttct agtctcttct gtctgttttc cagctctttt gctttctgct 6601 gctctttctg caatttccta atgtaatcca ctgatgcttt caggatggtg cctttattcc 6661 acctcatgtc tggatcattt gacttgggaa ttaaagtccc cagctcctta attctgtcat 6721 tgatattaaa ccgccttctt cgttcaatga ggttgtggtt gtccttcttt tgtctctctt 6781 ttgccagggc tctgacttct gcttctactg gaaagccatc aggtctttga taagagtcaa 6841 acttgccaca tggtccagct ttgtccatta tgtgcatcat gcccggagat ggagtaacgg 6901 ataattccct tttgacggcc ggcaggcttg aggggcagga gttactgatg gaaactccag 6961 ctggaggaag agcatgattg ctgtacatgt ccagcaggtt tgctgaaact ggaatcgtgt 7021 ttgtcatttg aagtcctgca tccatgaacc caagaatgtc atcactgtag cttgattcca 7081 aacttataat gtcttcaatt acatcgtcca tctctttctc acagttgagg gtgagaaggg 7141 ccatagggct gttgggagcg ctggctccgg gtcccggggt catgccgtgc tcggcgggct 7201 ggctggggcc gggccctgtg ctggcctgtg ggctcagctt ggctcccagt gcactggaca 7261 ggtagtgctt cacctgctgc ctctggctct gctggatgtg gtacttcgag ggggtttcca 7321 ggtgggtctg aacctggtag tgactgtact cgagcatctc caacatgttc aactatgtgt 7381 tagcttcaaa aaagtattcc ctttgtgaat ttaggtgatg tgcaagaact gaccagtcgt 7441 cttggccagc tgaagatgtc aatgccaaag aaaagacaca aaatgtattt aagggggtgg 7501 gaggggattg ctcttctcat attgtttttt ttacccaaac gatgaattta tgcctttata 7561 aagttcattt gacgtcaccg gtccgattcg acaaaagcct ctttgagacg atgggttact 7621 agtgtctaaa ctgaaactgg cttacggcta actaacgttt catctttagt tccactcatc 7681 ctgcataaaa cattgtttgt tctcagcatc cggcatacaa tcactagcac tgttggtcta 7741 aaaacaagac agtttaagat ggagttatct ctgaaatgtt gaagagcaaa agataggaaa 7801 gagaactttg ttgtctgatg ggcctttggt aattgcattt ttttcttaag agttgaaata 7861 ttgtgcacac gtatgtaagt gcgcacaaac aaaatcacag acgtacagtt catattcaga 7921 ttcatattca tttttttttc tacaatgatg cctcatgtac atcatcttat tatcttacgt 7981 gtcatttaca gtcaaaaaaa aactagctgg ttgaataaaa gtaactttaa gtcagaattt 8041 gccagacgta tgaacacttt ggggcttgat tgtaagtctt tttttttttt ttgttgttgt 8101 tggatgtact ggcacaatta tcatcagagt aaatcagtaa ctgttgtgca tttgcacaga 8161 aagcatagaa atgaactgta gttaaaaaag ctaacaatca taaacaaagt agcatgtgca 8221 tatactgtac tgaatttttt ttaaataata attgacataa tcactggttg taatgtgtaa 8281 aaaatgagtc tgccttaacc caacttccac tctaaattgt gtgttgatgc tcatttccca 8341 aaagggctga attacgatag aaaagataag tcaacagttt gttgtctctg cattgtttga 8401 gttgactaca tcttgagcaa atgtagctat tgtaaaatgg ggatgagtat gtggtacaga 8461 gaaccatctc gtcaatggag tggaaactgc attaacatag agagatagag agaacagctg 8521 tgtctaggca tggggtataa gattctgatg gtatgataac ctttgataaa aatatcacag 8581 tttgatggta ttgtgattgc cactctaaaa tatattattt tttttaatgt cttagtaaaa 8641 aacaaaaact ttaaacataa tatattttat tttgagaaac attgaaaaaa tattttggaa 8701 cagaaaacat gtcaagctta ataatttcaa tgaatgatcg acttctgctg tcttcattag 8761 tttcaaaaac agatttcttt acaatttaaa aagcattttt gtatctcttt tctgctgaaa 8821 atgctgttgt cctaaaaaac aaaaaaataa aaaataaata aaaaatctta aatatacctt 8881 aggaacggta tagcagaaaa tattggtggt tttaaaccct tgactttttc aaaccggggt 8941 ataccttgaa aacggttatc gtcctatgcc tagctttgtc aacaaccaaa aagcaacttt 9001 attaacatct taaggcaagt ttaaaggcaa catctaaaac agaatgaacg gttggagttt 9061 agtgaagttt atttataaac taatttcgag agaatcacgt gcttatgact gactacagct 9121 ggtcccacat tagcctatgt atgattcacc aatcagacaa ttcctaactc atcataggcc 9181 gccagagttc ctttcatttc ctcgaaagag cggcacagca gcccagttgt tagcactgtt 9241 gcctcaaagc aagaacgtca ctggtccaag tctctaacag gccagtccat ggttctgtgt 9301 ggagtttaca tgttgtcctc gtgctcatgt ggatatcccc tgggttctcc ggttttctcc 9361 tacagtccaa aaacttgcga cataggtgaa ttgaacaaaa ccaaattagc atagacaagc 9421 tcctaatcag cagaatatct ctccgtagca atctcttatt cgagatctgc gaagatacgg 9481 ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga 9541 atcacatact tcctccattt tcttccactg attgactgtt ataatttccc taatttccag 9601 gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat 9661 gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac 9721 atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata 9781 gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta 9841 aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa 9901 ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa 9961 attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga 10021 tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg 10081 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 10141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 10201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 10261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 10321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 10381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 10441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 10501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 10561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 10621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 10681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 10741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 10801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 10861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 10921 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 10981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 11041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 11101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 11161 aggcattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 11221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 11281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 11341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 11401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 11461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 11521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 11581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 11641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 11701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 11761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 11821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 11881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 11941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 12001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 12061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 12121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 12181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 12241 atttccccga aaagtg //