LOCUS       Exported               11575 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  This plasmid expresses full-length human TET3..
ACCESSION   .
VERSION     .
KEYWORDS    FH-TET3-pEF
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li
            H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A
  TITLE     Modulation of TET2 expression and 5-methylcytosine oxidation by the 
            CXXC domain protein IDAX.
  JOURNAL   Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 
            2013 Apr 7.
  PUBMED    23563267
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        189..1367
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          420..1358
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1315..1335
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1384..1403
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1384..1402
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1435..1444
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1444..1467
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1480..1506
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4279..4290
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             6922..6963
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6973..6990
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7013..7030)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7019..7243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7289..7717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7376..7395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7586..7607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7726..7746)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7731..8060
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7911..8046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7973..7992
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8127..8921
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8181..8200)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8791..8810
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9097..9218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9134..9153)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9188..9207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9267..9283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9267..9283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9280..9302)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9291..9307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9315..9345)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9360..9381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9498..9515)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9658..10246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9738..9757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10417..11277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11040..11059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11278..11382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11457..11476)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
       61 gatatacgcg ttgacattga ttattgacta ggcttttgca aaaagctttg caaagatgga
      121 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
      181 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
      241 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
      301 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      361 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
      421 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
      481 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
      541 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
      601 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
      661 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
      721 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
      781 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
      841 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
      901 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
      961 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     1021 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     1081 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     1141 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     1201 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     1261 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     1321 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attagcttgg
     1381 tactaatacg actcactata gggagaccca agctggctag gtaagcttgg taccgccacc
     1441 atggactaca aggacgacga tgacaagctc gatggaggat acccctacga cgtgcccgac
     1501 tacgccggag gactcgacag ccagtttcag gtgcccctgg ccgtccagcc ggacctgcca
     1561 ggcctttatg acttccctca gcgccaggtg atggtaggga gcttcccggg gtctgggctc
     1621 tccatggctg ggagtgagtc ccaactccga gggggtggag atggtcgaaa gaaacggaaa
     1681 cggtgtggta cttgtgagcc ctgccggcgg ctggaaaact gtggcgcttg cactagctgt
     1741 accaaccgcc gcacgcacca gatctgcaaa ctgcgaaaat gtgaggtgct gaagaaaaaa
     1801 gtagggcttc tcaaggaggt ggaaataaag gctggtgaag gagccgggcc gtggggacaa
     1861 ggagcggctg tcaagacagg ctcagagctc agcccagttg atggacctgt tccaggtcag
     1921 atggactcag ggccagtgta ccatggggac tcacggcagc taagcgcctc aggggtgccg
     1981 gtcaatggtg ctagagagcc cgctggaccc agtctgctgg ggactggggg tccttggcgg
     2041 gtagaccaaa agcccgactg ggaggctgcc ccaggcccag ctcatactgc tcgcctggaa
     2101 gatgcccacg atctggtggc cttttcggct gtggccgaag ctgtgtcctc ttatggggcc
     2161 cttagcaccc ggctctatga aaccttcaac cgtgagatga gtcgtgaggc tgggaacaac
     2221 agcaggggac cccggccagg gcctgagggc tgctctgctg gcagcgaaga ccttgacaca
     2281 ctgcagacgg ccctggccct cgcgcggcat ggtatgaaac cacccaactg caactgcgat
     2341 ggcccagaat gccctgacta cctcgagtgg ctggagggga agatcaagtc tgtggtcatg
     2401 gaaggagggg aggagcggcc caggctccca gggcctctgc ctcctggtga ggccggcctc
     2461 ccagcaccaa gcaccaggcc actcctcagc tcagaggtgc cccagatctc tccccaagag
     2521 ggcctgcccc tgtcccagag tgccctgagc attgccaagg aaaaaaacat cagcttgcag
     2581 accgccattg ccattgaggc cctcacacag ctctcctctg ccctcccgca gccttctcat
     2641 tccacccccc aggcttcttg cccccttcct gaggccttgt cacctcctgc ccctttcaga
     2701 tctccccagt cttacctccg ggctccctca tggcctgtgg ttcctcctga agagcactca
     2761 tcttttgctc ctgatagctc tgccttccct ccagcaactc ctagaactga gttccctgaa
     2821 gcctggggca ctgacacccc tccagcaacg ccccggagct cctggcccat gcctcgccca
     2881 agccccgatc ccatggctga actggagcag ttgttgggca gcgccagtga ttacatccag
     2941 tcagtattca agcggcctga ggccctgcct accaagccca aggtcaaggt ggaggcaccc
     3001 tcttcctccc cggccccggc cccatcccct gtacttcaga gggaggctcc cacgccatcc
     3061 tcggagcccg acacccacca gaaggcccag accgccctgc agcagcacct ccaccacaag
     3121 cgcagcctct tcctagaaca ggtgcacgac acctccttcc ctgctccttc agagccttct
     3181 gctcctggct ggtggccccc accaagttca cctgtcccac ggcttccaga cagaccaccc
     3241 aaggagaaga agaagaagct cccaacacca gctggaggtc ccgtgggaac ggagaaagct
     3301 gcccctggga tcaagcccag tgtccgaaag cccattcaga tcaagaagtc caggccccgg
     3361 gaagcacagc ccctcttccc acctgtccga cagattgtcc tggaagggct taggtcccca
     3421 gcctcccagg aagtgcaggc tcatccaccg gcccctctgc ctgcctcaca gggctctgct
     3481 gtgcccctgc ccccagaacc ttctcttgcg ctatttgcac ctagtccctc cagggacagc
     3541 ctgctgcccc ctactcagga aatgaggtcc cccagcccca tgacagcctt gcagccaggc
     3601 tccactggcc ctcttccccc tgccgatgac aagctggaag agctcatccg gcagtttgag
     3661 gctgaatttg gagatagctt tgggcttccc ggcccccctt ctgtgcccat tcaggacccc
     3721 gagaaccagc aaacatgtct cccagcccct gagagcccct ttgctacccg ttcccccaag
     3781 caaatcaaga ttgagtcttc gggggctgtg actgtgctct caaccacctg cttccattca
     3841 gaggagggag gacaggaggc cacacccacc aaggctgaga acccactcac acccaccctc
     3901 agtggcttct tggagtcacc tcttaagtac ctggacacac ccaccaagag tctgctggac
     3961 acacctgcca agagagccca ggccgagttc cccacctgcg attgcgtcga acaaatagtg
     4021 gagaaagatg aaggtccata ttatactcac ttgggatctg gccccacggt cgcctctatc
     4081 cgggaactca tggaggagcg gtatggagag aaggggaaag ccatccggat cgagaaggtc
     4141 atctacacgg ggaaggaggg aaagagctcc cgcggttgcc ccattgcaaa gtgggtgatc
     4201 cgcaggcaca cgctggagga gaagctactc tgcctggtgc ggcaccgggc aggccaccac
     4261 tgccagaacg ctgtgatcgt catcctcatc ctggcctggg agggcattcc ccgtagcctc
     4321 ggagacaccc tctaccagga gctcaccgac accctccgga agtatgggaa ccccaccagc
     4381 cggagatgcg gcctcaacga tgaccggacc tgcgcttgcc aaggcaaaga ccccaacacc
     4441 tgtggtgcct ccttctcctt tggttgttcc tggagcatgt acttcaacgg ctgcaagtat
     4501 gctcggagca agacacctcg caagttccgc ctcgcagggg acaatcccaa agaggaagaa
     4561 gtgctccgga agagtttcca ggacctggcc accgaagtcg ctcccctgta caagcgactg
     4621 gcccctcagg cctatcagaa ccaggtgacc aacgaggaaa tagcgattga ctgccgtctg
     4681 gggctgaagg aaggacggcc cttcgcgggg gtcacggcct gcatggactt ctgtgcccac
     4741 gcccacaagg accagcataa cctctacaat gggtgcaccg tggtctgcac cctgaccaag
     4801 gaagacaatc gctgcgtggg caagattccc gaggatgagc agctgcatgt tctccccctg
     4861 tacaagatgg ccaacacgga tgagtttggt agcgaggaga accagaatgc aaaggtgggc
     4921 agcggagcca tccaggtgct caccgccttc ccccgcgagg tccgacgcct gcccgagcct
     4981 gccaagtcct gccgccagcg gcagctggaa gccagaaagg cagcagccga gaagaagaag
     5041 attcagaagg agaagctgag cactccggag aagatcaagc aggaggccct ggagctggcg
     5101 ggcattacgt cggacccagg cctgtctctg aagggtggat tgtcccagca aggcctgaag
     5161 ccctccctca aggtggagcc gcagaaccac ttcagctcct tcaagtacag cggcaacgcg
     5221 gtggtggaga gctactcggt gctgggcaac tgccggccct ccgaccctta cagcatgaac
     5281 agcgtgtact cctaccactc ctactatgca cagcccagcc tgacctccgt caatggcttc
     5341 cactccaagt acgctctccc gtcttttagc tactatggct ttccatccag caaccccgtc
     5401 ttcccctctc agttcctggg tcctggtgcc tgggggcata gtggcagcag tggcagtttt
     5461 gagaagaagc cagacctcca cgctctgcac aacagcctga gcccggccta cggtggtgct
     5521 gagtttgccg agctgcccag ccaggctgtt cccacagacg cccaccaccc cactcctcac
     5581 caccagcagc ctgcgtaccc aggccccaag gagtatctgc ttcccaaggc ccccctactc
     5641 cactcagtgt ccagggaccc ctcccccttt gcccagagct ccaactgcta caacagatcc
     5701 atcaagcaag agccagtaga cccgctgacc caggctgagc ctgtgcccag agacgctggc
     5761 aagatgggca agacacctct gtccgaggtg tctcagaatg gaggacccag tcacctttgg
     5821 ggacagtact caggaggccc aagcatgtcc cccaagagga ctaacggtgt gggtggcagc
     5881 tggggtgtgt tctcgtctgg ggagagtcct gccatcgtcc ctgacaagct cagttccttt
     5941 ggggccagct gcctggcccc ttcccacttc acagatggcc agtgggggct gttccccggt
     6001 gaggggcagc aggcagcttc ccactctgga ggacggctgc gaggcaaacc gtggagcccc
     6061 tgcaagtttg ggaacagcac ctcggccttg gctgggccca gcctgactga gaagccgtgg
     6121 gcgctggggg caggggattt caactcggcc ctgaaaggta gtcctgggtt ccaagacaag
     6181 ctgtggaacc ccatgaaagg agaggagggc aggattccag ccgcaggggc cagccagctg
     6241 gacagggcct ggcagtcctt tggtctgccc ctgggatcca gcgagaagct gtttggggct
     6301 ctgaagtcag aggagaagct gtgggacccc ttcagcctgg aggaggggcc ggctgaggag
     6361 ccccccagca agggagcggt gaaggaggag aagggcggtg gtggtgcgga ggaggaagag
     6421 gaggagctgt ggtcggacag tgaacacaac ttcctggacg agaacatcgg cggcgtggcc
     6481 gtggccccag cccacggctc catcctcatc gagtgtgccc ggcgggagct gcacgccacc
     6541 acgccgctta agaagcccaa ccgctgccac cccacccgca tctcgctggt cttctaccag
     6601 cacaagaacc tcaaccagcc caaccacggg ctggccctct gggaagccaa gatgaagcag
     6661 ctggcggaga gggcacgggc acggcaggag gaggctgccc ggctgggcct gggccagcag
     6721 gaggccaagc tctacgggaa gaagcgcaag tgggggggca ctgtggttgc tgagccccag
     6781 cagaaagaga agaagggggt cgtccccacc cggcaggcac tggctgtgcc cacagactcg
     6841 gcggtcaccg tgtcctccta tgcctacacg aaggtcactg gcccctacag ccgctggatc
     6901 tagtctagag ggcccttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     6961 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     7021 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7081 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     7141 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     7201 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     7261 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     7321 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7381 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7441 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7501 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7561 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7621 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7681 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     7741 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7801 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7861 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7921 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7981 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8041 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     8101 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     8161 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     8221 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     8281 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     8341 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     8401 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     8461 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     8521 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     8581 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     8641 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     8701 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     8761 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     8821 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     8881 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc
     8941 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     9001 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     9061 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     9121 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     9181 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     9241 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     9301 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     9361 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9421 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9481 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9541 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9601 ggaaagaaca tgtgagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    10141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    10201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    10261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    11041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    11101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    11161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    11221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    11281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11401 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
    11461 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    11521 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgaca
//
