LOCUS Exported 6811 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Mutational dissection of the G rich region of the c-kit promoter. ACCESSION . VERSION . KEYWORDS pC-KIT6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6811) AUTHORS Ducani C., Bernardinelli G., Hogberg B., Keppler B.K., Terenzi A. TITLE Interplay of three G-quadruplex units in the KIT promoter JOURNAL J. Am. Chem. Soc. 2019 REFERENCE 2 (bases 1 to 6811) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6811 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 1244..1253 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1250..2185 /codon_start=1 /product="Renilla luciferase" /label=hRluc /note="human codon-optimized" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal 2244..2292 /note="synthetic polyadenylation signal" promoter 2301..3052 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" regulatory 3082..3091 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3088..4740 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /db_xref="GI:42718124" /protein_id="AAS38485.1" /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" RBS 4047..4055 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" polyA_signal 4784..4905 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4795..4814) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4849..4868 /label=SV40pA-R /note="SV40 polyA, reverse primer" promoter 5038..5142 /gene="bla" /label=AmpR promoter CDS 5143..6003 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5361..5380) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6174..6762 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6663..6682 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttgctcacat ggctcgacag atctgaggga tgtcttatta gtttatcaga atgcataggg 61 aaagctcatc tttcttggag taggtcttcc aataagtgtt caagccctgg gtcttccctc 121 actacaataa taaacatacc tgctctaccc tcctcacaaa gctgttatcg tgagcatgaa 181 aagacgagtc gctgtgggga agaactatta acccctatac agatactgtt gttgctttcc 241 gttcaaggat ccaccatgag actgtgggta aggcactttc tgggcagtga aatcgggctt 301 aagagaatca ttgtactttt attttgctcc tgggaccggc aagggcagtt aagagcccta 361 gccctaaaac tgtcgtctgt tacagaggca gagaaatcat tactggggtt ggcagcgttg 421 gggggagggg ggaaaacgtg tatgaaaacc tgggctccca gagcaaactt cgccacgcgc 481 cctccatcgc ccctgtctcc acacaagtcc cggcaggggc ttcttgggcg gaggccacgc 541 cgaccaatag gaacccactg tgttcctaca ggttacgaag caggtggaga aattgagcag 601 aaacaattag cgaaaccggg ctcagccttt accgcggtgc caggagctcc taacaggcct 661 ggaggggaat gtcggggctc aatttcctaa cgctcccctc cccatcccca tgccacctcc 721 acgagcagcg gcgtccagcc tcctcccgcc cgaacgtgct cgaggggcgg gcagtcgacc 781 tttattgtct ggggagcacc tggcaggtgg cgggcccgtg ccctaacgtg tgcgtggtgc 841 ccagcttcac aaagcgagcg ggcagcacct ccttggtccg ggaacgcctc agcctggccg 901 tccacatccc aggggtggaa aggtggagag agaaaggggc tccggagtca agagcgggga 961 gagagggcgc gcgccctcct cctcccggcg ggcacagccc cccggcatta acacgtcgaa 1021 agagcagggg ccagacgccg ccgggaagaa gcgagaccca aacaaacgcg aaaaagaaaa 1081 ggcgaggagg ggcgtggccg gcgcgcagaa agagaacgct aagaggagga actgctgctc 1141 gccgctcgcg gctctggggg ctcggctttg ccgcgctcgc tgcacttggg cgagagctgg 1201 aacgtggacc agagctcgga tcccatcgca gctaccgcgg ctagccacca tggcttccaa 1261 ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg 1321 caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga agcacgccga 1381 gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt 1441 gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc 1501 cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg 1561 gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg 1621 tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga 1681 gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc 1741 cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac 1801 catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga 1861 gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc 1921 tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct 1981 tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa 2041 cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct 2101 ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga 2161 gcgcgtgctg aagaacgagc agtaattcta ggcgatcgct cgagcccggg aattcgttta 2221 aacctagagc ggccgctggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg 2281 gttttttgtg tgaggatcta aatgagtctt cggacctcgc gggggccgct taagcggtgg 2341 ttagggtttg tctgacgcgg ggggaggggg aaggaacgaa acactctcat tcggaggcgg 2401 ctcggggttt ggtcttggtg gccacgggca cgcagaagag cgccgcgatc ctcttaagca 2461 cccccccgcc ctccgtggag gcgggggttt ggtcggcggg tggtaactgg cgggccgctg 2521 actcgggcgg gtcgcgcgcc ccagagtgtg accttttcgg tctgctcgca gacccccggg 2581 cggcgccgcc gcggcggcga cgggctcgct gggtcctagg ctccatgggg accgtatacg 2641 tggacaggct ctggagcatc cgcacgactg cggtgatatt accggagacc ttctgcggga 2701 cgagccgggt cacgcggctg acgcggagcg tccgttgggc gacaaacacc aggacggggc 2761 acaggtacac tatcttgtca cccggaggcg cgagggactg caggagcttc agggagtggc 2821 gcagctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa 2881 tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg 2941 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc caggtccact tcgcatatta 3001 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aaaagcttgg 3061 cattccggta ctgttggtaa agccaccatg gccgatgcta agaacattaa gaagggccct 3121 gctcccttct accctctgga ggatggcacc gctggcgagc agctgcacaa ggccatgaag 3181 aggtatgccc tggtgcctgg caccattgcc ttcaccgatg cccacattga ggtggacatc 3241 acctatgccg agtacttcga gatgtctgtg cgcctggccg aggccatgaa gaggtacggc 3301 ctgaacacca accaccgcat cgtggtgtgc tctgagaact ctctgcagtt cttcatgcca 3361 gtgctgggcg ccctgttcat cggagtggcc gtggcccctg ctaacgacat ttacaacgag 3421 cgcgagctgc tgaacagcat gggcatttct cagcctaccg tggtgttcgt gtctaagaag 3481 ggcctgcaga agatcctgaa cgtgcagaag aagctgccta tcatccagaa gatcatcatc 3541 atggactcta agaccgacta ccagggcttc cagagcatgt acacattcgt gacatctcat 3601 ctgcctcctg gcttcaacga gtacgacttc gtgccagagt ctttcgacag ggacaaaacc 3661 attgccctga tcatgaacag ctctgggtct accggcctgc ctaagggcgt ggccctgcct 3721 catcgcaccg cctgtgtgcg cttctctcac gcccgcgacc ctattttcgg caaccagatc 3781 atccccgaca ccgctattct gagcgtggtg ccattccacc acggcttcgg catgttcacc 3841 accctgggct acctgatttg cggctttcgg gtggtgctga tgtaccgctt cgaggaggag 3901 ctgttcctgc gcagcctgca agactacaaa attcagtctg ccctgctggt gccaaccctg 3961 ttcagcttct tcgctaagag caccctgatc gacaagtacg acctgtctaa cctgcacgag 4021 attgcctctg gcggcgcccc actgtctaag gaggtgggcg aagccgtggc caagcgcttt 4081 catctgccag gcatccgcca gggctacggc ctgaccgaga caaccagcgc cattctgatt 4141 accccagagg gcgacgacaa gcctggcgcc gtgggcaagg tggtgccatt cttcgaggcc 4201 aaggtggtgg acctggacac cggcaagacc ctgggagtga accagcgcgg cgagctgtgt 4261 gtgcgcggcc ctatgattat gtccggctac gtgaataacc ctgaggccac aaacgccctg 4321 atcgacaagg acggctggct gcactctggc gacattgcct actgggacga ggacgagcac 4381 ttcttcatcg tggaccgcct gaagtctctg atcaagtaca agggctacca ggtggcccca 4441 gccgagctgg agtctatcct gctgcagcac cctaacattt tcgacgccgg agtggccggc 4501 ctgcccgacg acgatgccgg cgagctgcct gccgccgtcg tcgtgctgga acacggcaag 4561 accatgaccg agaaggagat cgtggactat gtggccagcc aggtgacaac cgccaagaag 4621 ctgcgcggcg gagtggtgtt cgtggacgag gtgcccaagg gcctgaccgg caagctggac 4681 gcccgcaaga tccgcgagat cctgatcaag gctaagaaag gcggcaagat cgccgtgtaa 4741 taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag 4801 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 4861 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 4921 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac 4981 ctctacaaat gtggtaaaat cgataaggat ccaggtggca cttttcgggg aaatgtgcgc 5041 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 5101 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 5161 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 5221 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 5281 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 5341 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 5401 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 5461 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 5521 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 5581 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 5641 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 5701 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 5761 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 5821 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 5881 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 5941 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 6001 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 6061 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 6121 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 6181 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 6241 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 6301 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 6361 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 6421 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 6481 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 6541 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 6601 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 6661 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 6721 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 6781 tttttacggt tcctggcctt ttgctggcct t //