LOCUS Exported 12940 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp. ACCESSION . VERSION . KEYWORDS pIA33 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12940) AUTHORS Muh U, Pannullo AG, Weiss DS, Ellermeier CD TITLE A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in Clostridioides difficile. JOURNAL J Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 10.1128/JB.00711-18. PUBMED 30745377 REFERENCE 2 (bases 1 to 12940) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12940 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 2272..6378 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" misc_RNA 7272..7347 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS complement(7742..8113) /codon_start=1 /gene="traJ" /product="oriT-recognizing protein" /label=traJ /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE KQDELGKVMMGVVRPRAEP" oriT 8146..8255 /note="incP origin of transfer" primer_bind complement(9451..9468) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9622..10210) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9702..9721) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ggcttagggt attttttttg tttaattcca atattggttc aaactcctgt gtaatacttt 61 tgacacttcc atctgtattg aattgacagt tgatatggta tctcattatt ggcttgttct 121 tatgtttttc tatgtattta ttttttactt tcaaataatt tgtatttcca tttttagtta 181 tgtttaacac actatcctta ttactcaata aaattatttt ttgttttaac atatgattta 241 tagaatctaa aaattgatat tgacttataa tagtactttc tacctctcta gctttttcta 301 gtgtttcaaa ttcttcaaac tctgctatat tgctatagta tagcttatca ttaataatat 361 tatctgacaa ctttaattcc attttataaa acaattccaa gttatgtata aaatttttaa 421 tgttacaaca tatagactta ttatttgttc ttatatggta tctccattta ctacttctag 481 ctctcatgtt atatgcacaa ctactcccaa cataaacata tattgcttgt tctaactcat 541 atttttgtaa aatttcaaca tcatatccag tcaaatcgta ctctctctta tatacaaatg 601 gtttttctaa ttttttgttc aataaatctc ctcctatttc ttacttaatt tagctaaaaa 661 tctatttata tttcacctcc tttttgactt taagcctacg aataccatat ttatgattat 721 aacatcttct atttttccca aatccttaca tctcccctaa tttttagact taagggcgaa 781 ttctgcatca agctagcata aaaataagaa gcctgcattt gcaggcttct tatttttatg 841 gtacgtataa tttaaagggt tatggctgta tatagtcata acccttatat ttcattaatt 901 aaagttaaat tttgtatatt caaaaagctc ttgatattta cggcagagcc accatataga 961 gatgcctcag accctaatga tgaaatttct atatttattc ctttgttaaa agagctaact 1021 aacatatctt ttgtaatctg taatatctca gggatatcac taattatttg gctatttaaa 1081 tatattattt caggagcata agttgtaata gcattattta tagctattgt taagtaacta 1141 caaaactcat gaataacttt tttggcattc tggttatctt cataatatag ttgctttact 1201 atatcagaat ctattttagg aatattttct aaagaagaaa gctgttcaaa tacctttttt 1261 tctgaacaat actgttctaa acagccacga tttccacaag gacatagttt accattaggc 1321 atgatgatag tgtgaccaat ttctccactc ataccatttc tgccactata taatttatta 1381 tttattataa tgcctgaacc aaatccactg tgaatactga gactaagtag tgagttatgt 1441 acagttgaaa aagtattctc agctaaagct gttaagtttg cttcattttc tatgtgaata 1501 ggaaagtcat actttttgct aaggatgcta tataagtcaa tctcattaag attgtaatat 1561 ggagtaaaca atactttatt ttcacaagta atcccatgta tagccaaagt aagaccaatt 1621 accttataag gagtgtctat tttggatatg ttataactat ttataatttc atctatcaat 1681 tgtataacat tgtctttact tacttgtata tctgttaatt tcttagaatt tataatagtt 1741 ccatctaagt aagatagaga agaaaatata tagtcgtatc caatgtccat acttaaagaa 1801 atccctgcac atttattaaa tactaataat atgggttttc ttccgccact atgagtacta 1861 tttccaattc ctatttcatg aactagagat tcatcaataa gttttttggt aatagcggat 1921 atagttgctt tattcaatcc tatagtagaa gctatacttg ccctagaaat aggaccattt 1981 tttataattt gttcaagtac caatctttca ttcatttctc gaatagtgta tttatcagta 2041 accaatttga tattcctcct taaaataata ttgtaatact ttttacacaa aaataaaagg 2101 ttattttgca ttgacaaaga taattaaata ttttattatt agttcataag ttagtttaat 2161 atactaacaa aaataaagca agtaaaatat acctaaaata taaaaaaatt aggataggaa 2221 aacgatagtt atgaagtggc attcaaggag ggtaaagagg agagtcgacg catggataaa 2281 aaatatagta taggattagc aataggaaca aatagtgtag gatgggcagt aataactgat 2341 gaatataaag taccaagtaa aaaatttaaa gtattaggaa atacagatag acatagtata 2401 aaaaaaaatt taataggtgc attacttttt gatagtggtg aaacagcaga agcaacaaga 2461 cttaaaagaa cagcaagaag aagatatact agaagaaaaa atagaatatg ttatttacaa 2521 gaaatattta gtaatgaaat ggcaaaagta gatgatagtt tttttcatag attagaagaa 2581 agttttttag tagaagaaga taaaaaacac gaaagacatc caatatttgg aaatatagta 2641 gatgaagtag catatcatga aaaatatcca acaatatatc atttaagaaa aaaacttgta 2701 gatagtacag ataaagcaga tttaagactt atatatcttg cattagcaca tatgataaaa 2761 tttagaggac attttcttat agaaggtgat ttaaatccag ataatagtga tgtagataaa 2821 ctttttatac aattagtaca aacatataat caattatttg aagaaaatcc aataaatgca 2881 agtggtgtag atgcaaaagc aatattaagt gcaagattaa gtaaaagtag aagattagaa 2941 aatcttatag cacaattacc tggagaaaaa aaaaatggat tatttggtaa tcttatagct 3001 ttaagtttag gattaacacc aaattttaaa tcaaattttg atcttgcaga agatgcaaaa 3061 cttcaattaa gtaaagatac atatgatgat gatttagata atttacttgc acaaataggt 3121 gatcaatatg cagatttatt tcttgcagca aaaaatcttt ctgatgcaat acttttaagt 3181 gatatactta gagtaaatac agaaataaca aaagcaccat tatctgcaag tatgataaaa 3241 agatatgatg aacatcatca agatttaaca ttacttaaag cattagtaag acaacaatta 3301 ccagaaaaat ataaagaaat tttttttgat caaagtaaaa atggatatgc aggatatata 3361 gatggtggtg caagtcaaga agaattttat aaatttatta aaccaatatt agaaaaaatg 3421 gatggaacag aagaattatt agtaaaactt aatagagaag atttacttag aaaacaaaga 3481 acatttgata atggaagtat accacatcaa atacatttag gtgaattaca tgcaatatta 3541 agaagacaag aagattttta tccatttctt aaagataata gagaaaaaat tgaaaaaata 3601 cttacattta gaataccata ttatgtagga ccattagcaa gaggaaatag tagatttgct 3661 tggatgacaa gaaaaagtga agaaacaata acaccatgga attttgaaga agtagtagat 3721 aaaggagcat ctgcacaaag ttttatagaa agaatgacaa attttgataa aaatcttcca 3781 aatgaaaaag tattaccaaa acatagttta ttatatgaat attttacagt atataatgaa 3841 ttaacaaaag taaaatatgt aacagaaggt atgagaaaac cagcattttt aagtggtgaa 3901 caaaaaaaag ctatagtaga tttattattt aaaacaaata gaaaagtaac agtaaaacaa 3961 ttaaaagaag attattttaa aaaaattgaa tgttttgata gtgtagaaat atctggtgta 4021 gaagatagat ttaatgcaag tttaggaaca tatcatgatt tacttaaaat aataaaggat 4081 aaagattttt tagataatga agaaaatgaa gatattttag aagatatagt attaacatta 4141 actttatttg aagatagaga aatgatagaa gaaagattaa aaacttatgc tcatttattt 4201 gatgataaag taatgaaaca acttaaaaga agaagatata caggttgggg aagattaagt 4261 agaaaactta taaatggaat aagagataaa caaagtggaa aaacaatatt agattttctt 4321 aaaagtgatg gatttgcaaa tagaaatttt atgcaattaa tacatgatga tagtttaaca 4381 tttaaagaag atattcaaaa agcacaagta agtggacaag gtgatagttt acatgaacat 4441 atagcaaatt tagcaggatc tccagcaata aaaaaaggta tattacaaac agtaaaagta 4501 gttgatgaat tagtaaaagt aatgggtaga cataaaccag aaaatatagt tatagaaatg 4561 gcaagagaaa atcaaacaac acaaaaagga caaaaaaata gtagagaaag aatgaaaaga 4621 atagaagaag gtataaaaga attaggaagt caaatactta aagaacatcc agtagaaaat 4681 acacaacttc aaaatgaaaa actttattta tattatttac aaaatggaag agatatgtat 4741 gtagatcaag aattagatat aaatagatta agtgattatg atgtagatgc aatagtacca 4801 caaagttttc ttaaagatga ttctatagat aataaagtat taacaagatc tgataaaaat 4861 agaggtaaaa gtgataatgt accaagtgaa gaagttgtaa aaaaaatgaa aaattattgg 4921 agacaattac ttaatgcaaa acttataaca caaagaaaat ttgataatct tacaaaagca 4981 gaaagaggtg gattatctga attagataaa gctggattta taaaaagaca attagtagaa 5041 actagacaaa taacaaaaca tgtagcacaa atattagata gtagaatgaa tacaaaatat 5101 gatgaaaatg ataagcttat aagagaagta aaagttataa cattaaaaag taaattagta 5161 agtgatttta gaaaagattt tcaattttat aaagtaagag aaataaataa ttatcatcat 5221 gcacatgatg catatcttaa tgcagtagta ggaacagcat taataaaaaa atatcctaaa 5281 ttagaatctg aatttgtata tggagattat aaagtatatg atgtaagaaa aatgatagca 5341 aaaagtgaac aagaaatagg aaaagcaaca gcaaagtact ttttttattc taatataatg 5401 aattttttta agacagaaat aactttagca aatggtgaaa taagaaaaag acctttaata 5461 gaaactaatg gtgaaacagg tgaaatagta tgggataaag gtagagattt tgcaacagta 5521 agaaaagtat taagtatgcc acaagtaaat atagtaaaaa aaacagaagt acaaactgga 5581 ggatttagta aagaaagtat acttccaaaa agaaattcag ataaacttat tgcaagaaaa 5641 aaagattggg atccaaaaaa atatggtgga tttgatagtc caacagtagc atatagtgta 5701 ttagtagtag caaaagtaga aaaaggtaaa tctaaaaaac ttaaaagtgt aaaagaatta 5761 cttggaataa caataatgga aagaagtagt tttgaaaaaa atcctattga ttttttagaa 5821 gcaaaaggat ataaagaagt taaaaaagat ttaataataa aacttccaaa atattcttta 5881 tttgaattag aaaatggaag aaaaagaatg ttagcaagtg ctggtgaatt acaaaaaggt 5941 aatgaattag cattaccatc taaatatgta aattttcttt atttagcaag tcattatgaa 6001 aaacttaaag gatctcctga agataatgaa caaaaacaat tatttgtaga acaacataaa 6061 cattatttag atgaaataat tgaacaaata agtgaatttt ctaaaagagt aatattagca 6121 gacgcaaatt tagataaagt attatctgct tataataaac atagagataa accaataaga 6181 gaacaagcag aaaatataat acatcttttt acattaacaa atttaggtgc accagcagca 6241 tttaaatatt ttgatacaac aatagataga aaaagatata caagtacaaa agaagtatta 6301 gacgcaacat taatacatca aagtataaca ggattatatg aaacaagaat agatttatct 6361 caattaggtg gtgattaact cgagcaaaaa catcgtagaa atacggtgtt ttttgttacc 6421 ctaagtttca taaaaataag aagcctgcaa atgcaggctt cttattttta tggtttaaac 6481 ggttttagct gggatatcgg caaaacaagc aaatactact atagaagcag ttaaaagggc 6541 tattttttat aatctggagc atagacaaga agttagagaa gcaattaaaa aagaaaatat 6601 aagaaaaatt cttattattg ggacatcaaa aaaaatggtt aatcaaattg tagagagatt 6661 gtcaattgga agggtctgta agtttataaa tatacaagat ataagtacta aatatgaaat 6721 agaaatagca aaacaagcta gaaaagaagg aaatcatgta attcctgttc cagccgttga 6781 gataaaaagt atggcaagtg gtctcagtat aaattctttg aagagacttt ttagaaaagg 6841 aaataatagg aatatggtag ttttagaaaa aacaattata agaccaactt ttagttatat 6901 aggaaagttt tatattagtg ctgatgtaat taaacagatt attgaatatg aagtatacaa 6961 ctttggaagc attgatagaa taaataaaat caatatagag aattgcaata attttatgaa 7021 tatttttgta agtataaata tcaatgattt agaaactata aaaaaaattg aagacataca 7081 acagacaata aaaaagtcta ttgagaaaat gactttagtt aatgtacaaa aaatagatat 7141 taacattcat aaaattaaaa aaataaactt cctccaatgt taaaaaataa actgagaaaa 7201 tgatatacta atttgcaata aagtgtgcta taattaaact gtaaatggcc aaactttcag 7261 tttagcggtc tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa 7321 cttgaaaaag tggcaccgag tcggtgcttt ttttctatgg agaaatctag atcagcatga 7381 tgtctgacta gcggccgcgg atcctataag ttttaataaa actttaaata gaaaaaggct 7441 tctctcatga gaagtctttt ttatttaaaa taaatataaa ataaaataga ggctataaat 7501 agcctctatt ttatgtgaga aatccctaaa taaaaagatg ccagtgtgct ggaattcgcc 7561 cttaggctgc gctagggacc tctttagctc cttggaagct gtcagtagta ggggatcggt 7621 cttgccttgc tcgtcggtga tgtacttcac cagctccgcg aagtcgctct tcttgatgga 7681 gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa 7741 gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct 7801 tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg 7861 ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc 7921 gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga 7981 cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc 8041 ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg 8101 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca 8161 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc 8221 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag 8281 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata 8341 ccgaaaaaat cgctataatg accccgaagc aggaagggcg aattctgcag tgcagatctg 8401 accggtctct gaaaatataa aaaccacaga ttgatactaa aaccttggtt gtgttgcttt 8461 tcggggctta aatcaaggaa aaatccttgt tttaagcctt tcaaaaagaa acacaaggtc 8521 tttgtactaa cctgtggtta tgtataaaat tgtagatttt agggtaacaa aaaacaccgt 8581 atttctacga tgtttttgct taaatacttg tttttagtta cagacaaacc tgaagttaac 8641 tatttatcaa ttcctgcaat tcgtttacaa aacggcaaat gtgaaatccg tcacatactg 8701 cgtgatgaac ttgaattgcc aaaggaagta taattttgtt atcttcttta taatatttcc 8761 ccatagtaaa aataggaatc aaataatcat atcctttctg caaattcaga ttaaagccat 8821 cgaaggttga ccacggtatc atagatacat taaaaatgtt ttccggagca tttggctttc 8881 cttccattct atgattgttt ccataccgtt gcgtatcact ttcataatct gctaaaaatg 8941 atttaaagtc agacttacac tcagtccaaa ggctggaaaa tgtttcagta tcattgtgaa 9001 atattgtata gcttggtatc atctcatcat atatccccaa ttcaccatct tgattgattg 9061 ccgtcctaaa ctctgaatgg cggtttacaa tcattgcaat ataataaagc attgcaggat 9121 atagtttcat tcccttttcc tttatttgtg tgatatccac tttaacggtc atgctgtagg 9181 tacaaggtac acttgcaaag tagtggtcaa aatactcttt tctgttccaa ctatttttat 9241 caattttttc aaataccatc taagttccct ctcaaattca agtttatcgc tctaatgaac 9301 aaagatatta taccacattt ttgtgaattt ttcaacttgc ccacttcgac tgcactcccg 9361 acttaataac ttcttgaaca cttgccgaaa aagaaaaact gccgggtacg tacccgggga 9421 tcgatccccg gccgagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 9481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 9541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 9601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 9661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 9721 cccttgaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 9781 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 9841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 9901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 9961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 10021 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 10081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 10141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 10201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 10261 ctcacgttaa gggattttgg tcatgaaatg caagtttcta actaacatac atcatagtta 10321 ctaaactatg gtaactataa ttttattaac tatattaagc actgattagt actataactc 10381 aatataagca tatcccctgt atcgtaacta gagaaccaaa cgacggaaaa agcgatatag 10441 ataagtttag atagtgcacc aattttttac ttttctattt tagaatctag catttccaat 10501 gcttactaat actatttttg atttttaact tgttttaaaa aagcacctac taattaggat 10561 tctagctcta tattctagtt atacattctc tatattctag ctctatattc tagttataca 10621 ttctctatat tctagctcta tattctagtt atacattctc tatattctag ctctatattc 10681 tagttataca ttctctatat tctagctcta tattctagtt atacattctc tatattctag 10741 ctctatattc tagttataca ttctctatat tctagctcta tattctagtt atacattctc 10801 taaaaaacat atatatatat actacatata tatgtacaat tattttacca aaagaaattt 10861 ttaaaacggc ttaatttcaa tactttgatg ccattttttt tttgcaattc tcgtacaatt 10921 attttaccaa aagaaatttt tattaattag tttttttgca taaaaatagg actagaaaat 10981 caattctaat cctatcaaaa atctacttgt ttaatctata tgtatagtac ataaatcctc 11041 tatcatttat tttagtttcc catacatcta aaattttagt atctaataaa gattctatca 11101 tatttgaaat tctttgctgt gttactccta aatcttttgc taattcctct tgattaattc 11161 ttaatatatt tccttttatt ttatcatctt tgaccaataa atcttgctta tatctcatat 11221 gagtatataa tcttaattct tcagttgaaa ttattcccca aattactgcc atggtaacaa 11281 agtaactaat agtatattgt ttatctatat tacatctaac ttcttttaca ttgtatgtat 11341 cttttattcc tagctttgta acaccttttg tttttgtaat ataatcatta tgttgaagtt 11401 cttttaatgt ttctcttaat gtcttttcac tcataacaat gtttttaact ttcttcttac 11461 gtttataggt tatcagctcc attatatcgt ctatgtttaa ttctctatct ttattgttaa 11521 gtaacacatt atagataaac aattgattac cagtcattat tttaacccct ttcctattct 11581 tatatttcaa atcctttgag tgcttatgtg gcatattgaa caaagtatct tcatcactat 11641 atataaaatc tgattctatt ttattccaac aatcactatt aaactcacaa cctgtacaac 11701 caaaattaac cgttttaagc ttctcataca tatacttgac ttgatattct agctcactat 11761 ttgaaagact attttgtgat atatagttcc attctctagc aacggatatt acttgactta 11821 aacttttatt cctcaatctt aaagtcacaa ctattttttg caaatctagg tttttatgtc 11881 catctttact accatttttt aaaatatcta ctatacaatt tggtagctta ctatctaata 11941 tatattttgt attagtatta cctactgttt ttacatcttt gcaatatttt ttagctaact 12001 tttctatgtc atatctatat atatttttgt cttggtgtat tatttttact tgtttagtag 12061 tatttttaat attatatgta taaggtactc ttaatacttg agtagataaa tttgcttgtt 12121 tatctgcacc caatttattt attaatgtat ttgtaacttc tgatactaac ttaatgttat 12181 tagttttatt aattagcaca taaacatgga atccatttcc actatcaact aggatatgat 12241 aatgtaatcc tatcttagta aataaattaa ttatatcttt ataattaaaa ccttgtccta 12301 attctttttt gtcaaaatca aatcctaatg catatctatt tattaagttt tcattggttc 12361 ctgcttttcc atcagttgta cataggttaa aaaatgtatt ttcataataa atattttttg 12421 cgaaggtaaa ttctactata tcatctatat ttttaaaaaa agttgtttta ctatattctt 12481 taaattcttc tccgagttct aattgtttac tattactttt attattttga aatactctaa 12541 catattcatt atcaataaga ttatttcctt gtcctatacc atctctaaat actgccatta 12601 gaaatttttt caacttatat tttgaatcta attgttccat atatttatta tctccttatc 12661 tagttttttt aagagataac actacattaa atatatactt attgactaat caacttttac 12721 aactattgaa taattttagc ttagtatgta taataatatt gttaagatgc taaaattgat 12781 agcaatatta ttatataact acagatactt aatcagttag accgatattg gagttggtag 12841 ctccataaac tcaattggta gttgagttgt cattgtctag tagtattatc tcattttgtt 12901 gttatcttaa aatattattt taaaaatact ctgcttggtc //