LOCUS       Exported               10460 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLD-puro-2A-rtTA-TcVA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, 
            Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, 
            Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J
  TITLE     A lentiviral-based functional proteomics approach identifies 
            chromatin remodelling complexes important for the induction of 
            pluripotency.
  JOURNAL   Mol Cell Proteomics. 2010 Mar 19. ():.
  PUBMED    20305087
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(523..542)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1019..1039
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1159..1224
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             1231..1977
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     LTR             2056..2289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2361..2482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2398..2417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2452..2471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2522..2657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2584..2603
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(2677..2696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2678..2696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2706..2723)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2706..2722)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2715..2737)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2864..3319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2951..2970)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3161..3182
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3345..3449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3450..4310
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3668..3687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4481..5069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4970..4989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5223..5240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5357..5378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5393..5423
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5431..5447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5436..5458
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5455..5471
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5455..5471
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5490..5510
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5492..5510
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5538..5764
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5765..5945
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5992..6117
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6610..6843
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7028..7072
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     7370..7390
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7810..7828
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7845..8159
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    7853..7871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7889..7907
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7924..7942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7960..7978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7996..8014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8031..8049
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8067..8085
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8132..8156
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8209..8333
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        8358..8388
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8442..9101
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8508..8527)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9443..9748
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9627..9646
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9789..9913)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             9993..10016
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             10053..10076
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             10089..10106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             10116..10136
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10149..10169
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10182..10247
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    10295..10412
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccatgaccga gtacaagccc
      541 accgtgcgcc tcgccacccg cgacgacgtg cccagggccg tccgcaccct cgccgccgct
      601 ttcgccgact accccgccac ccgccacacc gtcgatcccg accgccacat cgagcgggtc
      661 accgagctgc aagaactctt cctcacccgc gtcgggctcg acatcggcaa ggtgtgggtc
      721 gctgacgacg gcgccgcagt ggctgtctgg accacacccg agagcgtcga agctggggcg
      781 gtgttcgccg agatcggccc tcgcatggcc gagttgagcg gttcccggct ggccgctcag
      841 caacagatgg aaggcctcct ggctccacac cggcccaagg agcccgcatg gttcctggcc
      901 accgtcggcg tctctcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
      961 ggagtggagg ctgccgagcg cgccggggtg cccgccttcc tggagacctc cgcaccccgc
     1021 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtgga ggtgcccgaa
     1081 ggaccacgca cctggtgcat gacccgcaag cccggtgccc tgcagggatc tggcgaagcc
     1141 agacacaaac agaaaattgt gaaacagact ttgaattttg accttctcaa gttggcggga
     1201 gacgtggagt ccaacccagg gcccgctagc atgtctagac tggacaagag caaagtcata
     1261 aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc
     1321 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     1381 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg
     1441 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc
     1501 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     1561 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     1621 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     1681 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     1741 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     1801 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac
     1861 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     1921 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaaatg
     1981 catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     2041 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     2101 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     2161 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     2221 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     2281 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     2341 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     2401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2461 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     2521 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     2581 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     2641 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     2701 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     2761 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     2821 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     2881 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2941 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3001 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3061 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3121 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3181 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3241 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3301 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3421 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3481 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3541 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3601 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3661 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3721 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3781 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3841 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3901 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3961 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4021 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4081 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4141 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4201 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4261 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4321 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4381 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4441 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4501 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4561 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4621 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4681 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4741 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4801 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4861 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4921 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4981 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5041 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5101 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5161 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5221 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     5281 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     5341 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     5401 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     5461 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     5521 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     5581 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     5641 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     5701 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     5761 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5821 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5881 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5941 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     6001 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     6061 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     6121 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     6181 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     6241 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     6301 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     6361 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     6421 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     6481 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     6541 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     6601 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     6661 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     6721 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     6781 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     6841 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     6901 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     6961 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     7021 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     7081 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     7141 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     7201 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     7261 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     7321 tcgacggtat cgatcacgag actagcctcg agcggccgcc cccttcaccg agggcctatt
     7381 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
     7441 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     7501 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgccaagtac
     7561 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     7621 cttatgggac tttcctactt ggcagtacat ctacattatt gaagcattta tcagggttat
     7681 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7741 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     7801 acctataaaa ataggcgtat cacgaggccc tttcgtcttc actcgagttt actccctatc
     7861 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     7921 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     7981 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     8041 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     8101 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     8161 ggagaattcg agctcggtac ccggggatcc tctagtcagc tgacgcgtac aagtttgtac
     8221 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     8281 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
     8341 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     8401 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     8461 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     8521 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     8581 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     8641 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     8701 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     8761 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     8821 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     8881 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     8941 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     9001 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     9061 aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac
     9121 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     9181 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     9241 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     9301 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     9361 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     9421 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     9481 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     9541 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     9601 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     9661 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     9721 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     9781 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     9841 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     9901 tgtacaaagt ggttgattct agagcgatcg ccagagagct gttcaacctg ctgggcgaga
     9961 accagccccc cgtggtgatc aaggcgatcg cctggagcca ccctcagttc gagaagggag
    10021 gaggaagcgg cggaggcagc ggaggaggaa gctggagcca cccgcagttc gagaaaggcg
    10081 gaagcggcca tcaccatcac catcaccctg caggagaaaa tctgtacttc caggggagag
    10141 gaggcagcga gaacctgtac tttcaggggc ctgcaggaag agactacaaa gaccatgacg
    10201 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagtag ttcgaattct
    10261 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10321 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10381 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    10441 cactttggcc gcggctcgag
//
