LOCUS       Exported                 629 bp ds-DNA     linear   SYN 08-JUL-2021
DEFINITION  MoClo golden gate assembly CD part for TetR (tet repressor; 
            identical to C2d but without ssrA degradation tag). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    C27m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 629)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2..625
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(75..94)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 aatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
       61 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      121 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      181 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      241 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      301 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      361 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      421 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      481 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      541 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      601 acttaaatgt gaaagtgggt cctaaaggt
//
