LOCUS       Exported                 704 bp ds-DNA     linear   SYN 10-JUL-2021
DEFINITION  MoClo golden gate assembly CD part for mScarlet (RFP variant; see 
            https://www.fpbase.org/protein/mscarlet/). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    C59m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 704)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             5..700
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(138..156)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aatgatggtg agcaagggcg aggcagtgat caaggagttc atgcggttca aggtgcacat
       61 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      121 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttctcctg
      181 ggacatcctg tcccctcagt tcatgtacgg ctccagggcc ttcaccaagc accccgccga
      241 catccccgac tactataagc agtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      301 cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc tccctggagg acggcaccct
      361 gatctacaag gtgaagctcc gcggcaccaa cttccctcct gacggccccg taatgcagaa
      421 gaaaacaatg ggctgggaag cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa
      481 gggcgacatt aagatggccc tgcgcctgaa ggacggcggc cgctacctgg cggacttcaa
      541 gaccacctac aaggccaaga agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa
      601 gttggacatc acctcccaca acgaggacta caccgtggtg gaacagtacg aacgctccga
      661 gggccgccac tccaccggcg gcatggacga gctgtacaag aggt
//
