LOCUS Exported 117 bp ds-DNA linear SYN 07-JUL-2021 DEFINITION MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS U25m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 117) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 117) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 7, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..117 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA 7..56 /label=sTRSV HHRz /note="hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)" RBS 86..108 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" ORIGIN 1 tactagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc 61 tacaaataat tttgtttaaa ataattttgt ttaactttaa gaaggagata tacaatg //