LOCUS Exported 110 bp ds-DNA linear SYN 11-JUL-2021 DEFINITION MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS UC16m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 110) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 110) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 11, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..110 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA 25..100 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" ORIGIN 1 tactaacttt cagtttagcg gtctgtttta gagctagaaa tagcaagtta aaataaggct 61 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttaggt //