LOCUS       Exported                 110 bp ds-DNA     linear   SYN 03-JUL-2021
DEFINITION  MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. 
            pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA 
            structure and open targeting region)..
ACCESSION   .
VERSION     .
KEYWORDS    UC20m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 110)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 3, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_RNA        25..100
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tactctcctc ctccccccca atatgtttta gagctagaaa tagcaagtta aaataaggct
       61 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttaggt
//
