LOCUS Exported 2695 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cre-conditional expression of the SthK channel. ACCESSION . VERSION . KEYWORDS pAAV-Syn-FLEX-Sthk-P2A-bPAC_minWPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2695) AUTHORS Bernal Sierra YA, Rost BR, Pofahl M, Fernandes AM, Kopton RA, Moser S, Holtkamp D, Masala N, Beed P, Tukker JJ, Oldani S, Bonigk W, Kohl P, Baier H, Schneider-Warme F, Hegemann P, Beck H, Seifert R, Schmitz D TITLE Potassium channel-based optogenetic silencing. JOURNAL Nat Commun. 2018 Nov 5;9(1):4611. doi: 10.1038/s41467-018-07038-8. PUBMED 30397200 REFERENCE 2 (bases 1 to 2695) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2695 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 166..621 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(253..272) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 463..484 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(638..657) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 757..779 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(817..835) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 903..1007 /gene="bla" /label=AmpR promoter CDS 1008..1868 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1226..1245) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2039..2627 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2528..2547 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gcgctcgctc gctcactgag gccgcccggg cgtcgggcga cctttggtcg cccggcctca 61 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg 121 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg 181 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 241 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 301 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 361 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga 421 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 481 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga 541 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 601 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat 661 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 721 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 781 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 841 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 901 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 961 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 1021 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 1081 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 1141 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 1201 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 1261 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 1321 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 1381 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 1441 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 1501 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1561 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1621 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1681 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1741 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1801 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1861 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1921 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1981 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 2041 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 2101 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 2161 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 2221 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 2281 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 2341 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2401 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2461 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2521 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2581 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2641 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtcc tgcag //