LOCUS Exported 10667 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses EB1-EGFP in mammalian cells. ACCESSION . VERSION . KEYWORDS pLenti-EB1-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10667) TITLE pLenti-EB1-EGFP JOURNAL Unpublished REFERENCE 2 (bases 1 to 10667) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10667 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 382..580 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 598..778 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 825..950 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1443..1676 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1861..1905 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2203..2320 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2367..3545 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2598..3536 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3493..3513 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 3563..3572 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4394..5113 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 4394..5110 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4439..4460) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4700..4719) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5047..5068 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5123..5711 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5176..5196) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5594..5605) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6235..6415 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6441..6458) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6447..6671 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6717..7145 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6804..6823) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7014..7035 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7154..7174) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7159..7488 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7339..7474 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7401..7420 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7536..7583 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7602..7976 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8106..8227 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8143..8162) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8197..8216 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8276..8292) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8276..8292) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8289..8311) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8300..8316 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8324..8354) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8369..8390 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8507..8524) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8678..9266) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8758..8777) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9437..10297) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10060..10079 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10298..10402) /gene="bla" /label=AmpR promoter primer_bind complement(10477..10496) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg 601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga 841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa 901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc 961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat 1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg 1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc 1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc 1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa 1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat 1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta 1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga 1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca 1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat 1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag 1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg 1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg 1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa 1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa 1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac 1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt 1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca 2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa 2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg 2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg 2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac 2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag 2341 cagagatcca gtttggttaa ttaattggct ccggtgcccg tcagtgggca gagcgcacat 2401 cgcccacagt ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa 2461 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 2521 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 2581 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 2641 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc 2701 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt 2761 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt 2821 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat 2881 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc 2941 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca 3001 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc 3061 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg 3121 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc 3181 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac 3241 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt 3301 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag 3361 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag 3421 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat 3481 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt 3541 cgtgaggaat tgggccatta cggccaccat ggcagtgaac gtatactcaa cgtcagtgac 3601 cagtgataac ctaagtcgac atgacatgct ggcctggatc aatgagtctc tgcagttgaa 3661 tctgacaaag atcgaacagt tgtgctcagg ggctgcgtat tgtcagttta tggacatgct 3721 gttccctggc tccattgcct tgaagaaagt gaaattccaa gctaagctag aacacgagta 3781 catccagaac ttcaaaatat tacaagcagg ttttaagaga atgggtgttg acaaaataat 3841 tcctgtggac aaattagtaa aaggaaagtt tcaggacaat tttgaattcg ttcagtggtt 3901 caagaagttc ttcgatgcaa actatgatgg aaaagactat gaccctgtgg ctgctagaca 3961 aggtcaagaa actgcagtgg ctccttccct tgttgctcca gctctgaata aaccgaagaa 4021 acctctcact tctagcagtg cagctcccca gaggcccatc tcaacacaga gaaccgctgc 4081 ggctcctaag gctggccctg gtgtggtgcg aaagaaccct ggtgtgggca acggagacga 4141 cgaggcagct gagttgatgc agcaggtcaa cgtattgaaa cttactgttg aagacttgga 4201 gaaagagagg gatttctact tcggaaagct acggaacatt gaattgattt gccaggagaa 4261 cgagggggaa aacgaccctg tattgcagag gattgtagac attctgtatg ccacagatga 4321 aggctttgtg atacctgatg aagggggccc acaggaggag caagaagagt atggccgcct 4381 cggccccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 4441 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 4501 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 4561 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 4621 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 4681 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 4741 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 4801 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 4861 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 4921 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 4981 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 5041 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 5101 gctgtacaag taaagcggcc gcaatcaacc tctggattac aaaatttgtg aaagattgac 5161 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 5221 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 5281 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 5341 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 5401 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 5461 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc 5521 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 5581 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 5641 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 5701 cgcctccccg cctgcctgca ggtttgtcga gacctagaaa aacatggagc aatcacaagt 5761 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 5821 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta 5881 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 5941 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag 6001 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta 6061 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta 6121 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt 6181 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct 6241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc 6421 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 6481 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 6541 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 6601 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 6661 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 6721 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 6781 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 6841 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 6901 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 6961 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 7021 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 7081 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 7141 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 7201 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 7261 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 7321 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 7381 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 7441 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 7501 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt 7561 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg 7621 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc 7681 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga 7741 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt 7801 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc 7861 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg 7921 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg 7981 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 8041 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 8101 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8161 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8221 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 8281 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 8341 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 8401 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 8461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 8521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 8581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 8641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 8701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 8761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 8821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 8881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 8941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 9001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 9061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 9121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 9181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 9241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 9301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 9361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 9421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 9481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 9541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 9601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 9661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 9721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 9781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 9841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 9901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 9961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 10021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 10081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 10141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 10201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 10261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 10321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 10381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 10441 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata 10501 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 10561 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt 10621 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt //