LOCUS Exported 10838 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Step-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open state. ACCESSION . VERSION . KEYWORDS pFUGW-GtACR1_C102A-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10838) AUTHORS Govorunova EG, Sineshchekov OA, Hemmati R, Janz R, Morelle O, Melkonian M, Wong GK, Spudich JL TITLE Extending the Time Domain of Neuronal Silencing with Cryptophyte Anion Channelrhodopsins. JOURNAL eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-0174-18. doi: 10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun. PUBMED 30027111 REFERENCE 2 (bases 1 to 10838) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10838 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 382..580 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 598..778 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 825..950 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1443..1676 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1861..1905 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2203..2320 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2412..3623 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3460..3479 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" CDS 4552..5268 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4594..4615) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4855..4874) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5202..5223 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5293..5881 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5346..5366) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5764..5775) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5884..5900) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5885..5901) /label=pBluescriptKS /note="For pBluescript vector" LTR 6406..6586 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6612..6629) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6618..6842 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6888..7316 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6975..6994) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7185..7206 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7325..7345) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7330..7659 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7510..7645 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7572..7591 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7707..7754 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7773..8147 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8277..8398 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8314..8333) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8368..8387 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8447..8463) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8447..8463) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8460..8482) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8471..8487 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8495..8525) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8540..8561 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8678..8695) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8849..9437) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8929..8948) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9608..10468) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10231..10250 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10469..10573) /gene="bla" /label=AmpR promoter primer_bind complement(10648..10667) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg 601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga 841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa 901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc 961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat 1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg 1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc 1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc 1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa 1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat 1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta 1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga 1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca 1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat 1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag 1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg 1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg 1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa 1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa 1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac 1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt 1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca 2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa 2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg 2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg 2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac 2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag 2341 cagagatcca gtttggttaa tcgcatgcta gctagttcga accttaatta acccgtgtcg 2401 gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac 2461 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac 2521 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa 2581 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg 2641 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg 2701 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc 2761 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg 2821 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt 2881 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt 2941 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag 3001 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt 3061 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat 3121 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg 3181 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc 3241 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc 3301 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg 3361 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt 3421 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta 3481 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat 3541 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc 3601 cgtttttggc ttttttgtta gacgaagctt gggctgcagg tcgactctag aggatccatg 3661 agcagcatca cctgtgatcc cgccatctac ggcgaatgga gcagggagaa ccagttctgc 3721 gtggagaaga gcctgatcac cctggacggc atcaagtacg tccagctggt gatggccgtc 3781 gtgagcgcct gtcaggtgtt cttcatggtg accagagccc ccaaggtgcc ctgggaagcc 3841 atctacctgc ccaccaccga gatgatcacc tattccctgg ccttcaccgg aaacggctac 3901 atcagagtgg ccaatggcaa gtacctgccc tgggccagaa tggccagctg gctgtgcacc 3961 gcccctatca tgctgggcct ggtgagcaat atggccctcg tgaagtacaa gagcatcccc 4021 ctcaacccta tgatgatcgc cgcctccagc atctgcaccg tgttcggcat caccgcctcc 4081 gtggtgctag acccgctgca cgtgtggctg tactgtttca tcagcagcat cttcttcatc 4141 ttcgagatgg tggtggcctt cgccattttc gccattacca tccacgattt ccagaccatc 4201 ggctccccca tgtccctgaa ggtggtggag aggctgaagc tgatgaggat cgtgttctac 4261 gtgagctgga tggcctaccc tatcctgtgg agcttctcca gcaccggcgc ctgcatcatg 4321 agcgagaaca ccagcagcgt gctgtacctg ctgggcgacg ctctgtgcaa gaacacctac 4381 ggcatcctgc tgtgggctac aacctggggc ctgctgaacg gcaagtggga cagggattac 4441 gtgaagggca ggaacgtgga cggcaccctg atgcctgagt acgagcagga cctggagaag 4501 ggcaacaccg agaggtacga ggacgccaga gccggcgaga ccgcggccgc cgtgagcaag 4561 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 4621 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 4681 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 4741 ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc 4801 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 4861 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 4921 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 4981 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 5041 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 5101 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 5161 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 5221 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaga attcgatatc 5281 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5341 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 5401 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 5461 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 5521 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 5581 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 5641 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt 5701 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 5761 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 5821 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 5881 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca 5941 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt 6001 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 6061 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat 6121 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca 6181 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt 6241 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg 6301 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc 6361 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta 6421 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 6481 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 6541 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac 6601 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 6661 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 6721 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 6781 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 6841 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 6901 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 6961 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 7021 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 7081 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 7141 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 7201 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 7261 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 7321 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 7381 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 7441 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 7501 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 7561 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 7621 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 7681 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca 7741 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg 7801 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct 7861 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca 7921 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg 7981 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct 8041 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg 8101 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag 8161 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 8221 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 8281 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 8341 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 8401 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 8461 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 8521 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 8581 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 8641 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 8701 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 8761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 8821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 8881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 8941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 9001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 9061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 9121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 9181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 9241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 9301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 9361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 9421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 9481 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 9541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 9601 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 9661 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 9721 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 9781 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 9841 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 9901 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 9961 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 10021 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 10081 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 10141 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 10201 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 10261 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 10321 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 10381 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 10441 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 10501 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 10561 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 10621 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 10681 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc 10741 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt 10801 tgcgctgctt cgcgatgtac gggccagata tacgcgtt //