LOCUS Exported 10790 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons. ACCESSION . VERSION . KEYWORDS pMCh2007_pLenti_Syn_mChe-cdc42E7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10790) AUTHORS Ciolli Mattioli C, Rom A, Franke V, Imami K, Arrey G, Terne M, Woehler A, Akalin A, Ulitsky I, Chekulaeva M TITLE Alternative 3' UTRs direct localization of functionally diverse protein isoforms in neuronal compartments. JOURNAL Nucleic Acids Res. 2018 Dec 22. pii: 5258023. doi: 10.1093/nar/gky1270. PUBMED 30590745 REFERENCE 2 (bases 1 to 10790) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10790 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" CDS 3159..3866 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(3304..3322) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 3572..3591 /label=mCherry-F /note="mCherry, forward primer" LTR 6595..6775 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6801..6818) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6807..7031 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7077..7505 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7164..7183) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7374..7395 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7514..7534) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7519..7848 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7699..7834 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7761..7780 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7896..7943 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7962..8336 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8466..8587 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8503..8522) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8557..8576 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8636..8652) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8636..8652) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8649..8671) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8660..8676 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8684..8714) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8729..8750 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8867..8884) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9038..9626) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9118..9137) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9797..10657) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10420..10439 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10658..10762) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acaagtatct gggaagggta acaatgcaag ttagcctgag 2641 gatttaggag gaggctgaaa aacagagtag gagccttact acgggtccag accctacgga 2701 caagaacccc cactcccact ccccaaattg cgcattccct cccccatcag agggggaggg 2761 gaagaggatg cagcgcggcg cggcgcgtgc gcactgtcgg atttagtacc gcggacagag 2821 ccttcgcccc cgctgccggc gcgcgccacc acctccccag caccaaaggc gggctgacgt 2881 cactctccag ccctccccaa actcccctac ctcaccgcct tggtcgcgtc cgtgcagcgg 2941 tgagtccagt cgggccgcac cacaagaggt gcaagatagg ggggtgcagg cgcgaccata 3001 cgctctgcgg cggcagagcc tcagcgctgc ctcagtctgc agcgggcagc agaggagtcg 3061 cgtcgtgcca gagagcgccg ccgtgctcct gagccccttg cgctccgccc ccgcggccca 3121 ccgacccact gccccttgga tctcgaggga attctaccat ggtgagcaag ggcgaggagg 3181 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 3241 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 3301 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 3361 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 3421 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 3481 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga 3541 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 3601 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 3661 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 3721 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 3781 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 3841 ccggcggcat ggacgagctg tacaagatgc agacaattaa gtgtgttgtt gttggtgatg 3901 gtgctgttgg taaaacatgt ctcctgatat cctacacaac aaacaaattc ccatcggaat 3961 atgtaccaac tgtttttgac aactatgcag tcacagttat gattggtgga gagccataca 4021 ctcttggact ttttgatact gcagggcaag aggattatga cagactacga ccgctaagtt 4081 atccacagac agatgttttt ctagtatgtt tctcagtggt ctctccatcc tcatttgaaa 4141 atgtgaaaga aaagtgggtg cctgagataa ctcaccactg tccaaagact cctttcttgc 4201 ttgttgggac ccaaattgat ctcagagatg acccctctac tattgagaaa cttgccaaga 4261 acaaacagaa gcctattact ccagagactg ctgaaaagct ggcgcgggat ctgaaggctg 4321 tcaagtatgt ggagtgctcc gccctcacac agaaaggcct aaagaatgtg tttgatgaag 4381 caatattggc tgccctggag cctccagaac cgaagaagag ccgcaggtgt gtgctgctat 4441 gaacgcatct ccagagccct ttctgcacag ctggtgttgt cgtcatacta aaagcaatgt 4501 ttaaatcaaa ctaaagatta aaaattaaaa tttgtttctg caataatgac aaacgccctg 4561 cacctaccca catgcactca tgtgagacaa ggcccattag tatggcccct ccttgccctt 4621 ccagtaatag ttaattttga ataattatgt attgtctgaa aagcaatgag tgctagtttt 4681 tgttttcttt gtttaaaaaa aaaaacaaaa atccccacaa acgtttgttg tttttttgtt 4741 taaaagcaag catgcttgta ttgactgtaa cagactaatt cagattgtca aagctgctcc 4801 ctggttccat tttggagagt aatctggggc atcttcgtgt cttctgtttt gttttgtttt 4861 gtttttcctc aacccttctc tccttttggg gtgtgtatgg ggttgtttct tagacttgta 4921 ttttaaaatt tgttaaccat aggacagcct gtggggagga ggacagagta tttccacatt 4981 ccactcccta ggtctagttt agcaaacgtg tccccacctg gtgctcttag gaaggagtat 5041 agaaacgcct cgattaacaa cgtattcctt cttgaaagtt gccttttctc tccacctctg 5101 agtgggtcgg cataatgatg gaactcatga aggtgggtgg acgctgtctc acccctcttt 5161 ctaggatgca ctctatatgt gactgtgact ttcaagggta tttgtttgcc atttcctgat 5221 ttagggaggg gaataatttc taacttcttt cattgataaa tgaaaagtat tgcacctttg 5281 aaatacacca aatggattga gttcctaatt aaaaaaagtt cttttgcccc tgtcagtcat 5341 tttcttacat gcttagcaca gattcacaac tcactgtgta gtgttcctag attcagccga 5401 agacctggta cctagggttc ctaacgggat ggtgttagga aacacatcat ggctgatgat 5461 tgccatctcc tttcaggtga tgaggtcaca ggcctgcttc cttaagaagc tatgagcatg 5521 gggtggggag catactttta accaacatgg ggaccagtca gggctatccc tttttttctt 5581 ccatgcctat gaggaacctt tgaacaactg gctgaagctt tcgcaagaaa aatggcgaga 5641 gcctcatgat tctttataag tgttaataga gactttatca gattattttg tttgcaggtt 5701 ccagttttta aaaaatgttt aggtaatctc cttccccagt cctgatcttg tggattcgtt 5761 agtgtcgaac caatgctttc ttgtgtctca actctttgta tatgaattct tttcagatgt 5821 attaaacaaa caaaaccctt cacaagtgag gtttaaacat cgattgattt acggcgctaa 5881 ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc 5941 gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac 6001 caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt 6061 gcgcctgctg gaagatggcg atggaccggt gacctagaaa aacatggagc aatcacaagt 6121 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 6181 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta 6241 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 6301 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag 6361 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta 6421 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta 6481 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt 6541 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct 6601 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6661 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6721 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc 6781 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 6841 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 6901 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 6961 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 7021 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 7081 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 7141 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 7201 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 7261 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 7321 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 7381 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 7441 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 7501 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 7561 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 7621 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 7681 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 7741 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 7801 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 7861 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt 7921 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg 7981 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc 8041 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga 8101 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt 8161 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc 8221 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg 8281 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg 8341 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 8401 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 8461 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8521 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8581 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 8641 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 8701 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 8761 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 8821 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 8881 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 8941 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 9001 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 9061 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 9121 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 9181 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 9241 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 9301 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 9361 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 9421 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 9481 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 9541 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 9601 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 9661 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 9721 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 9781 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 9841 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 9901 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 9961 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 10021 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 10081 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 10141 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 10201 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 10261 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 10321 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 10381 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 10441 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 10501 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 10561 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 10621 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 10681 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 10741 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac //