LOCUS Exported 2143 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.. ACCESSION . VERSION . KEYWORDS P1m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2143) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2143) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2143 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 51..71 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(77..94) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(248..836) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(328..347) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1007..1867) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1630..1649 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1868..1972) /gene="bla" /label=AmpR promoter primer_bind 2040..2058 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2062..2105 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2108..2129 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 ggagtttaca gctagctcag tcctaggtat tatgctagct actagagacc tactagtagc 61 ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 241 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 661 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 1081 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 1141 taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 1201 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 1261 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 1321 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 1381 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 1441 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 1501 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 1561 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 1621 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 1681 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 1741 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 1801 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 1861 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 1921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 1981 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 2041 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg 2101 atttctggaa ttcgcggccg cttctagaga ctagtgggtc tca //