LOCUS Exported 2270 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes logic). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS P16m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2270) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2270) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2270 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 144..160 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 178..198 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(204..221) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(375..963) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(455..474) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1134..1994) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1757..1776 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1995..2099) /gene="bla" /label=AmpR promoter primer_bind 2167..2185 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2189..2232 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2235..2256 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 ggagcggaac tcttgtgcgt ggcgcatgtc gttatcgcaa aaccgctgca cacttttgcg 61 cgacatgctc tgatccccct catctggggg ggcctatctg agggaattta cttgtgagcg 121 ctcacaattg ataatgagca caattgtgag cggataacaa ttgcattact agagacctac 181 tagtagcggc cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 241 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 301 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 361 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc 421 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 481 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 541 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 601 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 661 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 721 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 781 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 841 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 901 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 1021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1141 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1201 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1261 gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1321 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1381 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1441 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1501 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1561 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1621 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1681 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1741 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1801 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1861 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1921 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1981 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 2041 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2101 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2161 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct 2221 aaggatgatt tctggaattc gcggccgctt ctagagacta gtgggtctca //