LOCUS Exported 2178 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly AB for pLas-A-10b (Adam Meyer's improved pLas promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).. ACCESSION . VERSION . KEYWORDS P58m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2178) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2178) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2178 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 68..86 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 90..133 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 136..157 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 257..277 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(283..300) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(454..1042) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(534..553) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1213..2073) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1836..1855 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2074..2178) /gene="bla" /label=AmpR promoter ORIGIN 1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 61 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc 121 taaggatgat ttctggaatt cgcggccgct tctagagact agtgggtctc aggagaacga 181 tcgttggctg aactagcaaa tgagatagat ttcggtgaac ccggaccctt gctaggctcg 241 aagaatacta gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct 301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 421 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga 481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 1261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 1321 accatctggc cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt 1381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 1441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 1501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 1561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 1621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 1681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 1741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 1801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 1861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 1921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 1981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 2041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 2101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2161 acaaataggg gttccgcg //