LOCUS Exported 2239 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly AB for pSalTTTC (Adam Meyer's improved pSal promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).. ACCESSION . VERSION . KEYWORDS P60m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2239) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2239) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2239 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 68..86 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 90..133 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 136..157 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 318..338 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(344..361) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(515..1103) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(595..614) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1274..2134) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1897..1916 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2135..2239) /gene="bla" /label=AmpR promoter ORIGIN 1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 61 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc 121 taaggatgat ttctggaatt cgcggccgct tctagagact agtgggtctc aggagaacga 181 tcgttggctg ggggcctcgc ttgggttatt gctggtgccc ggccgggcgc aatattcatg 241 ttgatgattt attatatatc gagtggtgta tttatttata ttgtttgctc cgttaccgtt 301 attaactact agagacctac tagtagcggc cgctgcaggc ttcctcgctc actgactcgc 361 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 421 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg 541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 841 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 901 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 961 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 1021 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 1081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 1141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 1201 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 1261 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 1321 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 1381 taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg gctccagatt 1441 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 1501 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 1561 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 1621 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 1681 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 1741 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 1801 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 1861 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 1921 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 1981 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 2041 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 2101 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 2161 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 2221 aacaaatagg ggttccgcg //