LOCUS Exported 2178 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly AB part for pRpa-AM (Adam Meyer's improved pRPA promoter from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).. ACCESSION . VERSION . KEYWORDS P66m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2178) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2178) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2178 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 86..106 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(112..129) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(283..871) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(363..382) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1042..1902) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1665..1684 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1903..2007) /gene="bla" /label=AmpR promoter primer_bind 2075..2093 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2097..2140 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2143..2164 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 ggagaacgat cgttggctga cctgtccgat cggacaatat tacgcaagaa aatggtttgt 61 tactttcgaa taaatactag agacctacta gtagcggccg ctgcaggctt cctcgctcac 121 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 181 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc 301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 721 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 1021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 1081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 1141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc 1201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 1261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 1321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 1381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 1441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 1501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 1561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 1621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 1681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 1741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 1801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 1861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 1921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 1981 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 2041 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt 2101 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct 2161 agagactagt gggtctca //