LOCUS       Exported                2819 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  MoClo golden gate assembly CD part for Cerulean (modified CFP; 
            variant of https://www.fpbase.org/protein/cerulean/) - Please see 
            Supplemental Documents for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    C13m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2819)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(32..60)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     655..675
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    727..747
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(753..770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(924..1512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1004..1023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1683..2543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2306..2325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2544..2648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2716..2734
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2738..2781
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2784..2805
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
       61 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      121 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      181 tgtcactact ttgacttggg gtgttcaatg ctttgctaga tacccagatc atatgaaaca
      241 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      301 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      361 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      421 attggaatac aacgctattt cagataatgt atacatcact gcagacaaac aaaagaatgg
      481 aatcaaagct aatttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      541 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      601 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagatc acatggtcct
      661 tcttgagttt gtaacagctg ctgggattac actaggcatg gatgaactat acaaaaggta
      721 gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct gcgctcggtc
      781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      901 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1441 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1501 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1561 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1621 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1681 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1741 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1801 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1861 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1921 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1981 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2041 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2101 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2161 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2221 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2281 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2341 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2401 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2461 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2521 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2641 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2701 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2761 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctca
//
